Q19848 · VRK1_CAEEL
- ProteinSerine/threonine-protein kinase VRK1
- Genevrk-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids610 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Serine/threonine kinase that phosphorylates baf-1, thus regulating the association of baf-1 with chromatin and nuclear membrane proteins during nuclear envelope formation. May act through the egl-17 signaling pathway. Essential in hermaphrodites for formation of the vulva, uterus, and uterine seam cells and for development and maintenance of the somatic gonad and thus the germ line. Acts to prevent cep-1 from triggering an inappropriate cell cycle arrest, thereby promoting germ cell proliferation. Regulates anchor cell polarity and the timing of anchor cell invasion through the basement membranes separating vulval and somatic gonadal cells during the L3 larval stage.
Catalytic activity
- L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H+
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 38-46 | ATP (UniProtKB | ChEBI) | |||
Binding site | 61 | ATP (UniProtKB | ChEBI) | |||
Active site | 167 | Proton acceptor | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Cajal body | |
Cellular Component | chromatin | |
Cellular Component | cytoplasm | |
Cellular Component | nuclear envelope | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | histone H2AS1 kinase activity | |
Molecular Function | protein kinase activity | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Biological Process | cell fate specification | |
Biological Process | establishment or maintenance of cell polarity | |
Biological Process | gonad development | |
Biological Process | protein phosphorylation | |
Biological Process | regulation of fibroblast growth factor receptor signaling pathway | |
Biological Process | reproductive process | |
Biological Process | signal transduction |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSerine/threonine-protein kinase VRK1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ19848
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Nuclear during interphase, accumulates at the nuclear rim in prophase and localizes to chromatin throughout metaphase, anaphase and telophase.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Embryonically lethal. Homozygous animals produced by heterozygous hermaphrodites overcome this through maternal contribution. In these animals, defects in the formation of the vulva, uterus, and uterine seam cells and in development and maintenance of the somatic gonad and thus the germ line appear in the early L3 larval stage. Adults are sterile. Cells display mitotic defects, including impaired nuclear envelope formation and baf-1 delocalization.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 69 | Acts as a suppressor of lem-4l(ax475) mutant. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000405308 | 1-610 | Serine/threonine-protein kinase VRK1 | ||
Post-translational modification
Autophosphorylates in vitro.
Keywords
- PTM
Proteomic databases
Expression
Tissue specificity
Present in germ cells at all stages of progression from the mitotic zone to mature oocytes, but not in maturing spermatids (at the protein level). Expressed in the ventral nerve cord and vulva cells.
Gene expression databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
XENO | Q19848 | ANKLE2 Q86XL3 | 2 | EBI-2414048, EBI-1773621 | |
BINARY | Q19848 | baf-1 Q03565 | 3 | EBI-2414048, EBI-2535603 | |
BINARY | Q19848 | lem-4 H2KZB2 | 2 | EBI-2414048, EBI-6258914 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 32-384 | Protein kinase | |||
Region | 317-476 | Disordered | |||
Compositional bias | 325-401 | Basic and acidic residues | |||
Compositional bias | 409-430 | Basic and acidic residues | |||
Compositional bias | 431-476 | Polar residues | |||
Compositional bias | 498-518 | Polar residues | |||
Region | 498-530 | Disordered | |||
Region | 544-577 | Disordered | |||
Region | 590-610 | Disordered | |||
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length610
- Mass (Da)67,355
- Last updated1996-11-01 v1
- MD5 Checksum9D2CE6A0BAAFA6FD60C2E2298808DBB4
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 325-401 | Basic and acidic residues | |||
Compositional bias | 409-430 | Basic and acidic residues | |||
Compositional bias | 431-476 | Polar residues | |||
Compositional bias | 498-518 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FO080105 EMBL· GenBank· DDBJ | CCD61237.1 EMBL· GenBank· DDBJ | Genomic DNA |