Q19848 · VRK1_CAEEL

  • Protein
    Serine/threonine-protein kinase VRK1
  • Gene
    vrk-1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Serine/threonine kinase that phosphorylates baf-1, thus regulating the association of baf-1 with chromatin and nuclear membrane proteins during nuclear envelope formation. May act through the egl-17 signaling pathway. Essential in hermaphrodites for formation of the vulva, uterus, and uterine seam cells and for development and maintenance of the somatic gonad and thus the germ line. Acts to prevent cep-1 from triggering an inappropriate cell cycle arrest, thereby promoting germ cell proliferation. Regulates anchor cell polarity and the timing of anchor cell invasion through the basement membranes separating vulval and somatic gonadal cells during the L3 larval stage.

Catalytic activity

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site38-46ATP (UniProtKB | ChEBI)
Binding site61ATP (UniProtKB | ChEBI)
Active site167Proton acceptor

GO annotations

AspectTerm
Cellular ComponentCajal body
Cellular Componentchromatin
Cellular Componentcytoplasm
Cellular Componentnuclear envelope
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular Functionhistone H2AS1 kinase activity
Molecular Functionprotein kinase activity
Molecular Functionprotein serine kinase activity
Molecular Functionprotein serine/threonine kinase activity
Biological Processcell fate specification
Biological Processestablishment or maintenance of cell polarity
Biological Processgonad development
Biological Processprotein phosphorylation
Biological Processregulation of fibroblast growth factor receptor signaling pathway
Biological Processreproductive process
Biological Processsignal transduction

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Serine/threonine-protein kinase VRK1
  • EC number
  • Alternative names
    • Vaccinia-related kinase 1

Gene names

    • Name
      vrk-1
    • ORF names
      F28B12.3

Organism names

  • Taxonomic identifier
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    Q19848

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Cytoplasm
Nucleus, Cajal body
Note: Nuclear during interphase, accumulates at the nuclear rim in prophase and localizes to chromatin throughout metaphase, anaphase and telophase.

Keywords

Phenotypes & Variants

Disruption phenotype

Embryonically lethal. Homozygous animals produced by heterozygous hermaphrodites overcome this through maternal contribution. In these animals, defects in the formation of the vulva, uterus, and uterine seam cells and in development and maintenance of the somatic gonad and thus the germ line appear in the early L3 larval stage. Adults are sterile. Cells display mitotic defects, including impaired nuclear envelope formation and baf-1 delocalization.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis69Acts as a suppressor of lem-4l(ax475) mutant.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004053081-610Serine/threonine-protein kinase VRK1

Post-translational modification

Autophosphorylates in vitro.

Keywords

Proteomic databases

Expression

Tissue specificity

Present in germ cells at all stages of progression from the mitotic zone to mature oocytes, but not in maturing spermatids (at the protein level). Expressed in the ventral nerve cord and vulva cells.

Gene expression databases

Interaction

Binary interactions

Type
Entry 1Entry 2Number of experimentsIntAct
XENO Q19848ANKLE2 Q86XL32EBI-2414048, EBI-1773621
BINARY Q19848baf-1 Q035653EBI-2414048, EBI-2535603
BINARY Q19848lem-4 H2KZB22EBI-2414048, EBI-6258914

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain32-384Protein kinase
Region317-476Disordered
Compositional bias325-401Basic and acidic residues
Compositional bias409-430Basic and acidic residues
Compositional bias431-476Polar residues
Compositional bias498-518Polar residues
Region498-530Disordered
Region544-577Disordered
Region590-610Disordered

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    610
  • Mass (Da)
    67,355
  • Last updated
    1996-11-01 v1
  • MD5 Checksum
    9D2CE6A0BAAFA6FD60C2E2298808DBB4
MPPKKAPAKKLHELAAEVRVGHKINDISKKTFIVGKQFATGGFGRIHTCTEEGKSQQMVMKIEPSTNGPLLTEVVVFNRILKKELIESYKKKKKISWIGLPYLIANGYFTYESEKMRYMIIPKYATSLEAVRETNGGTLAMKDSLTVASCILDALEYLHESDYAHADVKAANILLEKQGVYSTAVLVDFGLAHRTTNNVDKPDKKRAHNGTCIFTSTDAHRGNNPSFRGDIEILAYNLMMWATGTLPWMALESSPEKVFDAKQKFIAGLPGTLQNVLKNESSAVVGCIASMFDISMKTNYTDKVDMGKLKKLVTDAIQKTSSDGKKTPTRQKKLAEEDKNAVTPKRSTRRLAVKEESDNKDNDEVEVKPEKKATPRKRTTRKAVEVNNDSDDNEEQYENPKSKSSRTQTKSKRAKEEDVDDEEEIIIPKSSRSRSKVQLSGEGSDVTTPSSAASTSRSRSIRLGLTSSTASSNKVAKKIEQKYKRLSMNKSSLVPVTISVASDKSPTTSTPSSSSGLRSKRKSSEDVGEGITLKTQVLITPAIKKAKTKSGISSATKASPTELRRVPGVRNFPKGRRSMIIKETSAKYKERLASRQTKPTFDDSSCSSEV

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias325-401Basic and acidic residues
Compositional bias409-430Basic and acidic residues
Compositional bias431-476Polar residues
Compositional bias498-518Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FO080105
EMBL· GenBank· DDBJ
CCD61237.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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