Q195N6 · PDRP1_MAIZE

  • Protein
    Pyruvate, phosphate dikinase regulatory protein, chloroplastic
  • Gene
    PDRP1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Bifunctional serine/threonine kinase and phosphorylase involved in the dark/light-mediated regulation of PPDK by catalyzing its phosphorylation/dephosphorylation. Dark/light-induced changes in stromal concentrations of the competing ADP and Pi substrates govern the direction of the reaction. In the dark, phosphorylates the catalytic intermediate of PPDK (PPDK-HisP), inactivating it. Light exposure induces the phosphorolysis reaction that reactivates PPDK. Phosphorylates PPDK at both Ser-528 and Thr-527 (PubMed:24710069).
Can use ADP as a high specificity substrate and GDP as a lower affinity substrate, but has no activity with UDP (PubMed:21414960).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Regulated by light/dark exposure.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
52 μMADP
1.2 μM[pyruvate, phosphate dikinase]
0.7 mMphosphate
0.7 μM[Pyruvate, phosphate dikinase] phosphate

pH Dependence

Optimum pH is 7.8-8.4 for both catalytic activities.

Pathway

Photosynthesis; C4 acid pathway.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site153-160ADP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast stroma
Molecular FunctionATP binding
Molecular Functionphosphotransferase activity, phosphate group as acceptor
Molecular Functionprotein serine/threonine kinase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyruvate, phosphate dikinase regulatory protein, chloroplastic
  • EC number
  • Alternative names
    • Bifunctional dikinase regulatory protein (BFRP)
    • Pyruvate, Pi dikinase regulatory protein (PPDK RP; PPDK regulatory protein)

Gene names

    • Name
      PDRP1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > PACMAD clade > Panicoideae > Andropogonodae > Andropogoneae > Tripsacinae > Zea

Accessions

  • Primary accession
    Q195N6

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-41Chloroplast
ChainPRO_000031539342-426Pyruvate, phosphate dikinase regulatory protein, chloroplastic

Proteomic databases

Expression

Tissue specificity

Leaf mesophyll-cells.

Gene expression databases

Interaction

Subunit

homodimer at pH 7.5 and homotetramer at pH 8.3.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region1-76Disordered
Compositional bias41-55Polar residues
Region94-124Disordered

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    426
  • Mass (Da)
    45,905
  • Last updated
    2006-07-11 v1
  • Checksum
    99F6F7B2B0D9F6D6
MIGCAKPLAAPLQAWARPPSPAGRRLPPSFCAPDTSPALTRAVESPGQSQSDDAPPPRSGEAASSLAPRASSHLDRWSRSRALRSGHRPALNRAALSSASVSAPPVIKSPRPEDAAVAAEDGEDDDVCEAERDAAAGKAIYIVSDGTGWTAEHSVNAALGQFENCLADRGCAVNTHLFSLIDDMDRLIEVIKQAAKEGALVLYTLADPSMAEATKKACDFWGVPCTDVLRPTVEAIASHIGVAPSGIPRSFPSRNGRLSEDYFQRIDAIDFTIKQDDGVLPQNFYRADIVLAGVSRTGKTPLSIYLAQKGYKVANVPIVMGVALPKSLFEINQDKVFGLTINPAILQGIRKTRAKTLGFDGRQSNYAEMDHVRQELVHANQIFVQNPWWPVIAVTGKAIEETAAVILGILHDRKQKCSMPRISKRY

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias41-55Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DQ645424
EMBL· GenBank· DDBJ
ABG00151.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

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