Q195N6 · PDRP1_MAIZE
- ProteinPyruvate, phosphate dikinase regulatory protein, chloroplastic
- GenePDRP1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids426 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Bifunctional serine/threonine kinase and phosphorylase involved in the dark/light-mediated regulation of PPDK by catalyzing its phosphorylation/dephosphorylation. Dark/light-induced changes in stromal concentrations of the competing ADP and Pi substrates govern the direction of the reaction. In the dark, phosphorylates the catalytic intermediate of PPDK (PPDK-HisP), inactivating it. Light exposure induces the phosphorolysis reaction that reactivates PPDK. Phosphorylates PPDK at both Ser-528 and Thr-527 (PubMed:24710069).
Can use ADP as a high specificity substrate and GDP as a lower affinity substrate, but has no activity with UDP (PubMed:21414960).
Can use ADP as a high specificity substrate and GDP as a lower affinity substrate, but has no activity with UDP (PubMed:21414960).
Catalytic activity
- ADP + N(tele)-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] = AMP + H+ + N(tele)-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase]
Cofactor
Activity regulation
Regulated by light/dark exposure.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
52 μM | ADP | |||||
1.2 μM | [pyruvate, phosphate dikinase] | |||||
0.7 mM | phosphate | |||||
0.7 μM | [Pyruvate, phosphate dikinase] phosphate |
pH Dependence
Optimum pH is 7.8-8.4 for both catalytic activities.
Pathway
Photosynthesis; C4 acid pathway.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast stroma | |
Molecular Function | ATP binding | |
Molecular Function | phosphotransferase activity, phosphate group as acceptor | |
Molecular Function | protein serine/threonine kinase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate, phosphate dikinase regulatory protein, chloroplastic
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > PACMAD clade > Panicoideae > Andropogonodae > Andropogoneae > Tripsacinae > Zea
Accessions
- Primary accessionQ195N6
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-41 | Chloroplast | ||||
Sequence: MIGCAKPLAAPLQAWARPPSPAGRRLPPSFCAPDTSPALTR | ||||||
Chain | PRO_0000315393 | 42-426 | Pyruvate, phosphate dikinase regulatory protein, chloroplastic | |||
Sequence: AVESPGQSQSDDAPPPRSGEAASSLAPRASSHLDRWSRSRALRSGHRPALNRAALSSASVSAPPVIKSPRPEDAAVAAEDGEDDDVCEAERDAAAGKAIYIVSDGTGWTAEHSVNAALGQFENCLADRGCAVNTHLFSLIDDMDRLIEVIKQAAKEGALVLYTLADPSMAEATKKACDFWGVPCTDVLRPTVEAIASHIGVAPSGIPRSFPSRNGRLSEDYFQRIDAIDFTIKQDDGVLPQNFYRADIVLAGVSRTGKTPLSIYLAQKGYKVANVPIVMGVALPKSLFEINQDKVFGLTINPAILQGIRKTRAKTLGFDGRQSNYAEMDHVRQELVHANQIFVQNPWWPVIAVTGKAIEETAAVILGILHDRKQKCSMPRISKRY |
Proteomic databases
Expression
Interaction
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-76 | Disordered | ||||
Sequence: MIGCAKPLAAPLQAWARPPSPAGRRLPPSFCAPDTSPALTRAVESPGQSQSDDAPPPRSGEAASSLAPRASSHLDR | ||||||
Compositional bias | 41-55 | Polar residues | ||||
Sequence: RAVESPGQSQSDDAP | ||||||
Region | 94-124 | Disordered | ||||
Sequence: AALSSASVSAPPVIKSPRPEDAAVAAEDGED |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length426
- Mass (Da)45,905
- Last updated2006-07-11 v1
- Checksum99F6F7B2B0D9F6D6
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 41-55 | Polar residues | ||||
Sequence: RAVESPGQSQSDDAP |
Keywords
- Technical term