Q18490 · TOST1_CAEEL
- ProteinProtein tost-1
- Genetost-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids142 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of the tost-1 variant of the PETISCO complex which plays an essential role in embryogenesis (PubMed:31147388, PubMed:31216475).
Within the complex acts as an adapter which binds to the complex via erh-2 (PubMed:31147388).
Does not seem to play a role in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) (PubMed:31147388).
May inhibit 21U-RNA accumulation (PubMed:31216475).
Required for chromosome segregation and cell division in early embryos (PubMed:31216475).
Within the complex acts as an adapter which binds to the complex via erh-2 (PubMed:31147388).
Does not seem to play a role in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) (PubMed:31147388).
May inhibit 21U-RNA accumulation (PubMed:31216475).
Required for chromosome segregation and cell division in early embryos (PubMed:31216475).
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 42 | May be required for interaction with erh-2 | ||||
Sequence: R |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Cellular Component | perinuclear region of cytoplasm | |
Cellular Component | RNA cap binding complex | |
Biological Process | cell division | |
Biological Process | chromosome segregation | |
Biological Process | embryo development ending in birth or egg hatching | |
Biological Process | positive regulation of cell division | |
Biological Process | positive regulation of chromosome segregation |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameProtein tost-1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ18490
Proteomes
Organism-specific databases
Subcellular Location
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
RNAi-mediated knockdown results in embryonic lethality (PubMed:31147388).
RNAi-mediated knockdown results in chromosome segregation and cell division defects in early embryos (PubMed:31216475).
RNAi-mediated knockdown enhances the activity of the PIWI-interacting RNA (piRNA) silencing pathway (PubMed:31147388).
RNAi-mediated knockdown disrupts the localization of tofu-6 to the perinuclear region of the germline (PubMed:31216475).
RNAi-mediated knockdown results in larger tofu-6-, erh-2- and pid-3-expressing foci (PubMed:31216475).
RNAi-mediated knockdown results in chromosome segregation and cell division defects in early embryos (PubMed:31216475).
RNAi-mediated knockdown enhances the activity of the PIWI-interacting RNA (piRNA) silencing pathway (PubMed:31147388).
RNAi-mediated knockdown disrupts the localization of tofu-6 to the perinuclear region of the germline (PubMed:31216475).
RNAi-mediated knockdown results in larger tofu-6-, erh-2- and pid-3-expressing foci (PubMed:31216475).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 42 | May abolish the interaction with erh-2. | ||||
Sequence: R → C |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000452469 | 1-142 | Protein tost-1 | |||
Sequence: MTTAIQYQVIPLADREHSNKVASILENKYKTATNKRITLNERFGVLEKGYTIQATEPETVKNDSDVFFVVHDRNLNSKKEFVENYLAKNPIVNSPEKVFEEEDKENAPTKKAVLKPSSTDEKKLTSSDIYIQEITRSILQSV |
Proteomic databases
Expression
Tissue specificity
Expressed in the germline.
Developmental stage
During early embryogenesis, expressed during prophase and interphase in 2- and 4-cell embryos.
Gene expression databases
Interaction
Subunit
Component of the tost-1 variant of the PETISCO complex (also called the pid-3, erh-2, tofu-6, and ife-3 small RNA complex) containing at least tost-1, tofu-6, ife-3, pid-3, and erh-2, which plays an essential role in embryogenesis (PubMed:31147388, PubMed:31216475).
Within the complex interacts with erh-2 (PubMed:31147388, PubMed:31216475).
Within the complex interacts with pid-3 and tofu-6 (PubMed:31216475).
In contrast to the pid-1 variant of the PETISCO complex, the tost-1 variant of the PETISCO complex plays a minor role in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) (PubMed:31147388).
Within the complex interacts with erh-2 (PubMed:31147388, PubMed:31216475).
Within the complex interacts with pid-3 and tofu-6 (PubMed:31216475).
In contrast to the pid-1 variant of the PETISCO complex, the tost-1 variant of the PETISCO complex plays a minor role in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) (PubMed:31147388).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q18490 | erh-2 Q20057 | 3 | EBI-21447173, EBI-21447087 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 97-122 | Basic and acidic residues | ||||
Sequence: KVFEEEDKENAPTKKAVLKPSSTDEK | ||||||
Region | 97-123 | Disordered | ||||
Sequence: KVFEEEDKENAPTKKAVLKPSSTDEKK |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length142
- Mass (Da)16,221
- Last updated1996-11-01 v1
- Checksum3394085F84A5C294
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 97-122 | Basic and acidic residues | ||||
Sequence: KVFEEEDKENAPTKKAVLKPSSTDEK |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX284603 EMBL· GenBank· DDBJ | CCD66786.1 EMBL· GenBank· DDBJ | Genomic DNA |