Q18490 · TOST1_CAEEL

  • Protein
    Protein tost-1
  • Gene
    tost-1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Component of the tost-1 variant of the PETISCO complex which plays an essential role in embryogenesis (PubMed:31147388, PubMed:31216475).
Within the complex acts as an adapter which binds to the complex via erh-2 (PubMed:31147388).
Does not seem to play a role in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) (PubMed:31147388).
May inhibit 21U-RNA accumulation (PubMed:31216475).
Required for chromosome segregation and cell division in early embryos (PubMed:31216475).

Features

Showing features for site.

1142102030405060708090100110120130140
TypeIDPosition(s)Description
Site42May be required for interaction with erh-2

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Cellular Componentperinuclear region of cytoplasm
Cellular ComponentRNA cap binding complex
Biological Processcell division
Biological Processchromosome segregation
Biological Processembryo development ending in birth or egg hatching
Biological Processpositive regulation of cell division
Biological Processpositive regulation of chromosome segregation

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Protein tost-1
  • Alternative names
    • Twenty one u antagonist 1

Gene names

    • Name
      tost-1
    • ORF names
      C35D10.13

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    Q18490

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Nucleus
Note: During early embryogenesis, localizes to the nucleus at prophase of cell division, and remains in the cytosol at interphase in 2- and 4-cell embryos (PubMed:31216475).
Mainly localizes to the germline cytoplasm (PubMed:31216475).

Keywords

Phenotypes & Variants

Disruption phenotype

RNAi-mediated knockdown results in embryonic lethality (PubMed:31147388).
RNAi-mediated knockdown results in chromosome segregation and cell division defects in early embryos (PubMed:31216475).
RNAi-mediated knockdown enhances the activity of the PIWI-interacting RNA (piRNA) silencing pathway (PubMed:31147388).
RNAi-mediated knockdown disrupts the localization of tofu-6 to the perinuclear region of the germline (PubMed:31216475).
RNAi-mediated knockdown results in larger tofu-6-, erh-2- and pid-3-expressing foci (PubMed:31216475).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis42May abolish the interaction with erh-2.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004524691-142Protein tost-1

Proteomic databases

Expression

Tissue specificity

Expressed in the germline.

Developmental stage

During early embryogenesis, expressed during prophase and interphase in 2- and 4-cell embryos.

Gene expression databases

Interaction

Subunit

Component of the tost-1 variant of the PETISCO complex (also called the pid-3, erh-2, tofu-6, and ife-3 small RNA complex) containing at least tost-1, tofu-6, ife-3, pid-3, and erh-2, which plays an essential role in embryogenesis (PubMed:31147388, PubMed:31216475).
Within the complex interacts with erh-2 (PubMed:31147388, PubMed:31216475).
Within the complex interacts with pid-3 and tofu-6 (PubMed:31216475).
In contrast to the pid-1 variant of the PETISCO complex, the tost-1 variant of the PETISCO complex plays a minor role in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) (PubMed:31147388).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q18490erh-2 Q200573EBI-21447173, EBI-21447087

Protein-protein interaction databases

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias97-122Basic and acidic residues
Region97-123Disordered

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    142
  • Mass (Da)
    16,221
  • Last updated
    1996-11-01 v1
  • Checksum
    3394085F84A5C294
MTTAIQYQVIPLADREHSNKVASILENKYKTATNKRITLNERFGVLEKGYTIQATEPETVKNDSDVFFVVHDRNLNSKKEFVENYLAKNPIVNSPEKVFEEEDKENAPTKKAVLKPSSTDEKKLTSSDIYIQEITRSILQSV

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias97-122Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BX284603
EMBL· GenBank· DDBJ
CCD66786.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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