Q16760 · DGKD_HUMAN
- ProteinDiacylglycerol kinase delta
- GeneDGKD
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1214 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:12200442, PubMed:23949095).
Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). By controlling the levels of diacylglycerol, regulates for instance the PKC and EGF receptor signaling pathways and plays a crucial role during development (By similarity).
May also regulate clathrin-dependent endocytosis (PubMed:17880279).
Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). By controlling the levels of diacylglycerol, regulates for instance the PKC and EGF receptor signaling pathways and plays a crucial role during development (By similarity).
May also regulate clathrin-dependent endocytosis (PubMed:17880279).
Catalytic activity
- a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
- 1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
- 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H+This reaction proceeds in the forward direction.
Pathway
Lipid metabolism; glycerolipid metabolism.
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameDiacylglycerol kinase delta
- EC number
- Short namesDAG kinase delta
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ16760
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Isoform 1
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
Disease & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 369 | Decreased interaction with AP2A2; when associated with A-372. | |||
Mutagenesis | 372 | Decreased interaction with AP2A2; when associated with A-369. | |||
Mutagenesis | 748 | Decreased interaction with AP2A2. | |||
Mutagenesis | 1145 | Loss of homooligomerization. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 1,210 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | ||
---|---|---|---|---|---|---|
Chain | PRO_0000218462 | 1-1214 | UniProt | Diacylglycerol kinase delta | ||
Modified residue (large scale data) | 44 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 666 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 668 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 731 | PRIDE | Phosphoserine | |||
Post-translational modification
Isoform 1
Phosphorylated (PubMed:12084710).
Phosphorylation by PKC induced by phorbol esters prevents homooligomerization and promotes association with membranes (PubMed:12084710).
Phosphorylation by PKC induced by phorbol esters prevents homooligomerization and promotes association with membranes (PubMed:12084710).
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Homooligomer (PubMed:12084710, PubMed:12200442).
Monomer (PubMed:12084710).
Interacts with AP2A2; regulates clathrin-dependent endocytosis (PubMed:17880279).
Monomer (PubMed:12084710).
Interacts with AP2A2; regulates clathrin-dependent endocytosis (PubMed:17880279).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | Q16760-2 | DGKD Q16760-2 | 5 | EBI-11147675, EBI-11147675 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain, zinc finger.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-24 | Pro residues | |||
Region | 1-47 | Disordered | |||
Region | 1-52 | Regulates association with membranes | |||
Domain | 53-146 | PH | |||
Zinc finger | 163-213 | Phorbol-ester/DAG-type 1 | |||
Zinc finger | 235-286 | Phorbol-ester/DAG-type 2 | |||
Domain | 317-451 | DAGKc | |||
Region | 554-584 | Disordered | |||
Region | 655-684 | Disordered | |||
Domain | 1145-1208 | SAM | |||
Domain
The SAM domain mediates homooligomerization.
The PH domain mediates association with membranes.
Sequence similarities
Belongs to the eukaryotic diacylglycerol kinase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q16760-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name2
- SynonymsDGKdelta2
- Length1,214
- Mass (Da)134,525
- Last updated2006-10-17 v4
- MD5 ChecksumF5C831FD99997BD0BCD97D7E032CB4E6
Q16760-2
- Name1
- SynonymsDGKdelta1
- Differences from canonical
- 1-52: MAAAAGAPPPGPPQPPPPPPPEESSDSEPEAEPGSPQKLIRKVSTSGQIRQK → MNMFLYFQ
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A096LNI6 | A0A096LNI6_HUMAN | DGKD | 163 | ||
C9JY42 | C9JY42_HUMAN | DGKD | 157 | ||
H7BZR7 | H7BZR7_HUMAN | DGKD | 451 | ||
H7C0L1 | H7C0L1_HUMAN | DGKD | 164 |
Features
Showing features for compositional bias, alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-24 | Pro residues | |||
Alternative sequence | VSP_012891 | 1-52 | in isoform 1 | ||
Sequence conflict | 511 | in Ref. 4; BAA11134 | |||
Sequence conflict | 828-834 | in Ref. 4; BAA11134 | |||
Sequence conflict | 960 | in Ref. 1; BAC11809 and 4; BAA11134 | |||
Sequence conflict | 1054 | in Ref. 4; BAA11134 | |||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB078966 EMBL· GenBank· DDBJ | BAC11809.1 EMBL· GenBank· DDBJ | mRNA | ||
D63479 EMBL· GenBank· DDBJ | BAA09766.3 EMBL· GenBank· DDBJ | mRNA | ||
D73409 EMBL· GenBank· DDBJ | BAA11134.1 EMBL· GenBank· DDBJ | mRNA | ||
BC006561 EMBL· GenBank· DDBJ | AAH06561.1 EMBL· GenBank· DDBJ | mRNA |