Q16649 · NFIL3_HUMAN
- ProteinNuclear factor interleukin-3-regulated protein
- GeneNFIL3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids462 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity).
Represses transcriptional activity of PER1 (By similarity).
Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity).
Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity).
Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity).
Protects pro-B cells from programmed cell death (By similarity).
Represses the transcription of CYP2A5 (By similarity).
Positively regulates the expression and activity of CES2 by antagonizing the repressive action of NR1D1 on CES2 (By similarity).
Required for the development of natural killer cell precursors (By similarity).
Represses transcriptional activity of PER1 (By similarity).
Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity).
Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity).
Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity).
Protects pro-B cells from programmed cell death (By similarity).
Represses the transcription of CYP2A5 (By similarity).
Positively regulates the expression and activity of CES2 by antagonizing the repressive action of NR1D1 on CES2 (By similarity).
Required for the development of natural killer cell precursors (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Community annotation
The subsequence ACRRKREFIPDEKKDAMYWEKRRKNNEAAKRSREKRRLNDLVLENKLIALGEENATLKAELLSLKLKFGLISSTAYAQEI, which contains the bZIP_2 domain, shows transcriptional repressor activity in a high-throughput recruitment assay.
Source | Submission date | Contributor |
---|---|---|
PubMed:33326746 | 0000-0002-4108-0575 |
Names & Taxonomy
Protein names
- Recommended nameNuclear factor interleukin-3-regulated protein
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ16649
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Disease & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 330 | Interacts with DR1 and partially affects transcriptional repression; when associated with E-332. | |||
Mutagenesis | 330 | Does not interact with DR1 and drastically affects transcriptional repression; when associated with E-332. | |||
Mutagenesis | 332 | Interacts with DR1 and partially affects transcriptional repression; when associated with E-330. | |||
Mutagenesis | 332 | Does not interact with DR1 and drastically affects transcriptional repression; when associated with E-330. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 400 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data), cross-link, modified residue.
Type | ID | Position(s) | Source | Description | ||
---|---|---|---|---|---|---|
Chain | PRO_0000292667 | 1-462 | UniProt | Nuclear factor interleukin-3-regulated protein | ||
Modified residue (large scale data) | 19 | PRIDE | Phosphoserine | |||
Cross-link | 24 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Modified residue (large scale data) | 182 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 207 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 210 | PRIDE | Phosphoserine | |||
Cross-link | 214 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 219 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternate | |||
Cross-link | 219 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate | |||
Modified residue (large scale data) | 285 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 286 | PRIDE | Phosphoserine | |||
Modified residue | 301 | UniProt | Phosphoserine | |||
Modified residue (large scale data) | 301 | PRIDE | Phosphoserine | |||
Cross-link | 306 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 314 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 326 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 332 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 337 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 350 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Modified residue (large scale data) | 352 | PRIDE | Phosphoserine | |||
Modified residue | 353 | UniProt | Phosphoserine | |||
Modified residue (large scale data) | 353 | PRIDE | Phosphoserine | |||
Cross-link | 360 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 394 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 401 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 406 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 412 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 419 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 424 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 434 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Cross-link | 448 | UniProt | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | |||
Modified residue (large scale data) | 461 | PRIDE | Phosphoserine | |||
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle.
Induction
Up-regulated by PHA or TPA.
Gene expression databases
Organism-specific databases
Interaction
Subunit
Homodimer (PubMed:1620116).
Binds DNA as a dimer (PubMed:1620116).
Interacts with DR1 (PubMed:8836190).
Interacts with PER2 and CRY2 (By similarity).
Interacts with NR0B2 (By similarity).
Interacts with MYSM1 (PubMed:24062447).
Binds DNA as a dimer (PubMed:1620116).
Interacts with DR1 (PubMed:8836190).
Interacts with PER2 and CRY2 (By similarity).
Interacts with NR0B2 (By similarity).
Interacts with MYSM1 (PubMed:24062447).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | Q16649 | AMOTL2 Q9Y2J4 | 4 | EBI-3951858, EBI-746752 | |
BINARY | Q16649 | AMOTL2 Q9Y2J4-4 | 3 | EBI-3951858, EBI-10187270 | |
BINARY | Q16649 | ATF1 P18846 | 3 | EBI-3951858, EBI-852794 | |
BINARY | Q16649 | CREB1 P16220 | 3 | EBI-3951858, EBI-711855 | |
BINARY | Q16649 | CREB3L3 Q68CJ9 | 2 | EBI-3951858, EBI-852194 | |
BINARY | Q16649 | DDIT3 P35638 | 2 | EBI-3951858, EBI-742651 | |
BINARY | Q16649 | GMCL1 Q96IK5 | 3 | EBI-3951858, EBI-2548508 | |
BINARY | Q16649 | HSF2BP O75031 | 3 | EBI-3951858, EBI-7116203 | |
BINARY | Q16649 | MAFF Q9ULX9 | 2 | EBI-3951858, EBI-721128 | |
BINARY | Q16649 | MAFG O15525 | 2 | EBI-3951858, EBI-713514 | |
XENO | Q16649 | MDV005 Q9DGW5 | 3 | EBI-3951858, EBI-10889526 | |
BINARY | Q16649 | NFIL3 Q16649 | 2 | EBI-3951858, EBI-3951858 | |
BINARY | Q16649 | PDE9A O76083-2 | 3 | EBI-3951858, EBI-11524542 | |
BINARY | Q16649 | PIH1D2 Q8WWB5 | 3 | EBI-3951858, EBI-10232538 | |
BINARY | Q16649 | RINT1 Q6NUQ1 | 3 | EBI-3951858, EBI-726876 | |
BINARY | Q16649 | TRAF2 Q12933 | 3 | EBI-3951858, EBI-355744 |
Complex viewer
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 73-136 | bZIP | |||
Region | 79-95 | Basic motif | |||
Region | 99-106 | Leucine-zipper | |||
Compositional bias | 189-214 | Polar residues | |||
Region | 189-237 | Disordered | |||
Compositional bias | 258-279 | Polar residues | |||
Region | 258-302 | Disordered | |||
Compositional bias | 280-294 | Basic and acidic residues | |||
Region | 299-363 | Necessary for transcriptional repression and sufficient for interaction with DR1 | |||
Sequence similarities
Belongs to the bZIP family. NFIL3 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length462
- Mass (Da)51,472
- Last updated2007-06-26 v2
- MD5 Checksum0D8193908B054B8C9FB18BD0930426B1
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 44-45 | in Ref. 1; AAA93067/CAA45597 and 2; AAB35410 | |||
Sequence conflict | 149 | in Ref. 4; CAG46846 | |||
Compositional bias | 189-214 | Polar residues | |||
Compositional bias | 258-279 | Polar residues | |||
Sequence conflict | 273 | in Ref. 1; AAA93067/CAA45597 and 2; AAB35410 | |||
Compositional bias | 280-294 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U26173 EMBL· GenBank· DDBJ | AAA93067.1 EMBL· GenBank· DDBJ | mRNA | ||
X64318 EMBL· GenBank· DDBJ | CAA45597.1 EMBL· GenBank· DDBJ | mRNA | ||
S79880 EMBL· GenBank· DDBJ | AAB35410.1 EMBL· GenBank· DDBJ | mRNA | ||
EF028070 EMBL· GenBank· DDBJ | ABK15691.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CR542049 EMBL· GenBank· DDBJ | CAG46846.1 EMBL· GenBank· DDBJ | mRNA | ||
AK313970 EMBL· GenBank· DDBJ | BAG36685.1 EMBL· GenBank· DDBJ | mRNA | ||
AL353764 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH471089 EMBL· GenBank· DDBJ | EAW62796.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC008197 EMBL· GenBank· DDBJ | AAH08197.1 EMBL· GenBank· DDBJ | mRNA |