Q15035 · TRAM2_HUMAN
- ProteinTranslocating chain-associated membrane protein 2
- GeneTRAM2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids370 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Necessary for collagen type I synthesis. May couple the activity of the ER Ca2+ pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca2+ concentration at the site of collagen synthesis, and a high Ca2+ concentration may be necessary for the function of molecular chaperones involved in collagen folding. Required for proper insertion of the first transmembrane helix N-terminus of TM4SF20 into the ER lumen, may act as a ceramide sensor for regulated alternative translocation (RAT) (PubMed:27499293).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Biological Process | collagen biosynthetic process | |
Biological Process | protein insertion into ER membrane | |
Biological Process | SRP-dependent cotranslational protein targeting to membrane, translocation |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameTranslocating chain-associated membrane protein 2
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ15035
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-22 | Cytoplasmic | ||||
Sequence: MAFRRRTKSYPLFSQEFVIHNH | ||||||
Transmembrane | 23-43 | Helical | ||||
Sequence: ADIGFCLVLCVLIGLMFEVTA | ||||||
Topological domain | 44-75 | Extracellular | ||||
Sequence: KTAFLFILPQYNISVPTADSETVHYHYGPKDL | ||||||
Transmembrane | 76-96 | Helical | ||||
Sequence: VTILFYIFITIILHAVVQEYI | ||||||
Topological domain | 97-119 | Cytoplasmic | ||||
Sequence: LDKISKRLHLSKVKHSKFNESGQ | ||||||
Transmembrane | 120-140 | Helical | ||||
Sequence: LVVFHFTSVIWCFYVVVTEGY | ||||||
Topological domain | 141-159 | Extracellular | ||||
Sequence: LTNPRSLWEDYPHVHLPFQ | ||||||
Transmembrane | 160-180 | Helical | ||||
Sequence: VKFFYLCQLAYWLHALPELYF | ||||||
Topological domain | 181-191 | Cytoplasmic | ||||
Sequence: QKVRKEEIPRQ | ||||||
Transmembrane | 192-209 | Helical | ||||
Sequence: LQYICLYLVHIAGAYLLN | ||||||
Topological domain | 210-214 | Extracellular | ||||
Sequence: LSRLG | ||||||
Transmembrane | 215-235 | Helical | ||||
Sequence: LILLLLQYSTEFLFHTARLFY | ||||||
Topological domain | 236-250 | Cytoplasmic | ||||
Sequence: FADENNEKLFSAWAA | ||||||
Transmembrane | 251-271 | Helical | ||||
Sequence: VFGVTRLFILTLAVLAIGFGL | ||||||
Topological domain | 272-287 | Extracellular | ||||
Sequence: ARMENQAFDPEKGNFN | ||||||
Transmembrane | 288-308 | Helical | ||||
Sequence: TLFCRLCVLLLVCAAQAWLMW | ||||||
Topological domain | 309-370 | Cytoplasmic | ||||
Sequence: RFIHSQLRHWREYWNEQSAKRRVPATPRLPARLIKRESGYHENGVVKAENGTSPRTKKLKSP |
Keywords
- Cellular component
Disease & Variants
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 347 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, glycosylation, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000185532 | 1-370 | UniProt | Translocating chain-associated membrane protein 2 | |||
Sequence: MAFRRRTKSYPLFSQEFVIHNHADIGFCLVLCVLIGLMFEVTAKTAFLFILPQYNISVPTADSETVHYHYGPKDLVTILFYIFITIILHAVVQEYILDKISKRLHLSKVKHSKFNESGQLVVFHFTSVIWCFYVVVTEGYLTNPRSLWEDYPHVHLPFQVKFFYLCQLAYWLHALPELYFQKVRKEEIPRQLQYICLYLVHIAGAYLLNLSRLGLILLLLQYSTEFLFHTARLFYFADENNEKLFSAWAAVFGVTRLFILTLAVLAIGFGLARMENQAFDPEKGNFNTLFCRLCVLLLVCAAQAWLMWRFIHSQLRHWREYWNEQSAKRRVPATPRLPARLIKRESGYHENGVVKAENGTSPRTKKLKSP | |||||||
Glycosylation | 55 | UniProt | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | |||||||
Modified residue (large scale data) | 346 | PRIDE | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 112-321 | TLC | ||||
Sequence: SKFNESGQLVVFHFTSVIWCFYVVVTEGYLTNPRSLWEDYPHVHLPFQVKFFYLCQLAYWLHALPELYFQKVRKEEIPRQLQYICLYLVHIAGAYLLNLSRLGLILLLLQYSTEFLFHTARLFYFADENNEKLFSAWAAVFGVTRLFILTLAVLAIGFGLARMENQAFDPEKGNFNTLFCRLCVLLLVCAAQAWLMWRFIHSQLRHWREY | ||||||
Region | 348-370 | Disordered | ||||
Sequence: YHENGVVKAENGTSPRTKKLKSP |
Sequence similarities
Belongs to the TRAM family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length370
- Mass (Da)43,328
- Last updated1996-11-01 v1
- Checksum9B5183F1A3D45366
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D31762 EMBL· GenBank· DDBJ | BAA06540.2 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
AK291751 EMBL· GenBank· DDBJ | BAF84440.1 EMBL· GenBank· DDBJ | mRNA | ||
AL049611 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH471081 EMBL· GenBank· DDBJ | EAX04373.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC028121 EMBL· GenBank· DDBJ | AAH28121.1 EMBL· GenBank· DDBJ | mRNA |