Q12WS1 · DADD_METBU
- Protein5'-deoxyadenosine deaminase
- GenedadD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids434 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the deamination of three SAM-derived enzymatic products, namely 5'-deoxyadenosine, S-adenosyl-L-homocysteine, and 5'-methylthioadenosine, to produce the inosine analogs. Can also deaminate adenosine. The preferred substrate for this enzyme is 5'-deoxyadenosine, but all these substrates are efficiently deaminated. Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. May also be involved in the recycling of 5'-deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens.
Miscellaneous
SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine. 5'-deoxyadenosine is a radical SAM enzyme reaction product which strongly inhibits radical SAM enzymes. A pathway for removing this product must be present in methanogens where the MTA/SAH nucleosidase which normally metabolizes this compound is absent.
Catalytic activity
- 5'-deoxyadenosine + H+ + H2O = 5'-deoxyinosine + NH4+This reaction proceeds in the forward direction.
Cofactor
Note: Binds 1 zinc ion per subunit.
Pathway
Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 63 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 65 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 92 | substrate | ||||
Sequence: E | ||||||
Binding site | 184 | substrate | ||||
Sequence: H | ||||||
Binding site | 211 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 214 | substrate | ||||
Sequence: E | ||||||
Binding site | 299 | substrate | ||||
Sequence: D | ||||||
Binding site | 299 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2'-deoxyadenosine deaminase activity | |
Molecular Function | 5'-deoxyadenosine deaminase activity | |
Molecular Function | 5'-methylthioadenosine deaminase activity | |
Molecular Function | adenosine deaminase activity | |
Molecular Function | metal ion binding | |
Molecular Function | S-adenosylhomocysteine deaminase activity | |
Biological Process | S-adenosylmethionine biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5'-deoxyadenosine deaminase
- EC number
- Short names5'-dA deaminase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Stenosarchaea group > Methanomicrobia > Methanosarcinales > Methanosarcinaceae > Methanococcoides
Accessions
- Primary accessionQ12WS1
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000312470 | 1-434 | 5'-deoxyadenosine deaminase | |||
Sequence: MADIIIKNGYVLTMDPEVDDIPNGVVVIEGGKIVEVAETTSATANTVIDAQGGVVMPGFVNTHTHAGMTLFRGYADDLPLAQWLQEHIWPAEAELTASDVLAGTRLACLEMIKSGTIAFADMYFFMEEVGKAVEECGLRAALSYGMIELWDDEKGTNELKKGREFVKEWNGKAEGRISVMYGPHAPNTCSKEFLSKVKEQAIADNVKIHIHVLETEAELNQMKEQYGMCSVNMLDTIDFFGPGVLAAHCIWLSDGDMDILADNNVNIAHNPVSNMKLASGVAPVMKLLDKGANVCLGTDGCASNNNLDMFDEMKTAALLQKVDTMDPTALPAKQVLEMATVNGAKALDINSGVLRKDYNADVIIIDMNKAHLSPLFDVPSQLVYSATGNDVRTTIVNGVVLMDERKVLCMNEQQVINDAKQAASDLVSRVDAKN |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length434
- Mass (Da)47,118
- Last updated2006-08-22 v1
- Checksum35ACF6BB854AC603
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000300 EMBL· GenBank· DDBJ | ABE52105.1 EMBL· GenBank· DDBJ | Genomic DNA |