Q12V31 · NADE_METBU
- ProteinNH(3)-dependent NAD(+) synthetase
- GenenadE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids263 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.
Catalytic activity
- deamido-NAD+ + NH4+ + ATP = AMP + diphosphate + NAD+ + H+
Pathway
Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (ammonia route): step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 29-36 | ATP (UniProtKB | ChEBI) | |||
Binding site | 35 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 114 | deamido-NAD+ (UniProtKB | ChEBI) | |||
Binding site | 134 | ATP (UniProtKB | ChEBI) | |||
Binding site | 139 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 147 | deamido-NAD+ (UniProtKB | ChEBI) | |||
Binding site | 154 | deamido-NAD+ (UniProtKB | ChEBI) | |||
Binding site | 163 | ATP (UniProtKB | ChEBI) | |||
Binding site | 185 | ATP (UniProtKB | ChEBI) | |||
Binding site | 244-245 | deamido-NAD+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | glutaminase activity | |
Molecular Function | metal ion binding | |
Molecular Function | NAD+ synthase (glutamine-hydrolyzing) activity | |
Molecular Function | NAD+ synthase activity | |
Biological Process | NAD biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNH(3)-dependent NAD(+) synthetase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Stenosarchaea group > Methanomicrobia > Methanosarcinales > Methanosarcinaceae > Methanococcoides
Accessions
- Primary accessionQ12V31
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000077575 | 1-263 | NH3-dependent NAD+ synthetase | ||
Interaction
Structure
Sequence
- Sequence statusComplete
- Length263
- Mass (Da)28,578
- Last updated2006-08-22 v1
- MD5 Checksum5208B8C76EC8408133E69328036CA9B1
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000300 EMBL· GenBank· DDBJ | ABE52695.1 EMBL· GenBank· DDBJ | Genomic DNA |