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Q12V31 · NADE_METBU

Function

function

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.

Catalytic activity

Pathway

Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (ammonia route): step 1/1.

Features

Showing features for binding site.

126320406080100120140160180200220240260
TypeIDPosition(s)Description
Binding site29-36ATP (UniProtKB | ChEBI)
Binding site35Mg2+ (UniProtKB | ChEBI)
Binding site114deamido-NAD+ (UniProtKB | ChEBI)
Binding site134ATP (UniProtKB | ChEBI)
Binding site139Mg2+ (UniProtKB | ChEBI)
Binding site147deamido-NAD+ (UniProtKB | ChEBI)
Binding site154deamido-NAD+ (UniProtKB | ChEBI)
Binding site163ATP (UniProtKB | ChEBI)
Binding site185ATP (UniProtKB | ChEBI)
Binding site244-245deamido-NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionglutaminase activity
Molecular Functionmetal ion binding
Molecular FunctionNAD+ synthase (glutamine-hydrolyzing) activity
Molecular FunctionNAD+ synthase activity
Biological ProcessNAD biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NH(3)-dependent NAD(+) synthetase
  • EC number

Gene names

    • Name
      nadE
    • Ordered locus names
      Mbur_1809

Organism names

Accessions

  • Primary accession
    Q12V31

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10000775751-263NH3-dependent NAD+ synthetase

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the NAD synthetase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    263
  • Mass (Da)
    28,578
  • Last updated
    2006-08-22 v1
  • MD5 Checksum
    5208B8C76EC8408133E69328036CA9B1
MDIIQAKDIIIDFIGTKLEGTGIEGAVVGISGGIDSALVAYLSVEALGAENVLGIHMPEASTPKSEIEDASKVAEALGIDFKVINITNVLEVYRTAMPDIDGASAHVDGNLKARIRMSMLYYYANMFGRVVMGTGNKSEILLGYFTKYGDGGVDIEPIGDLYKTEVREMSKMLGVPESILEKAPSAGLWEGQTDEDDLGVTYETIDKVLQPILAGEGQERVHLKLGVPMEEISSILLRVRSNLHKRTTPQIAYLDDLRGDWLS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000300
EMBL· GenBank· DDBJ
ABE52695.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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