Q12UT1 · FEN_METBU

Function

function

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity).

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.

Features

Showing features for binding site.

133850100150200250300
TypeIDPosition(s)Description
Binding site27Mg2+ 1 (UniProtKB | ChEBI)
Binding site80Mg2+ 1 (UniProtKB | ChEBI)
Binding site152Mg2+ 1 (UniProtKB | ChEBI)
Binding site154Mg2+ 1 (UniProtKB | ChEBI)
Binding site173Mg2+ 2 (UniProtKB | ChEBI)
Binding site175Mg2+ 2 (UniProtKB | ChEBI)
Binding site236Mg2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function5'-3' exonuclease activity
Molecular Function5'-flap endonuclease activity
Molecular FunctionDNA binding
Molecular Functionmagnesium ion binding
Biological ProcessDNA repair
Biological ProcessDNA replication, removal of RNA primer

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Flap endonuclease 1
  • EC number
  • Short names
    FEN-1
  • Alternative names
    • Flap structure-specific endonuclease 1

Gene names

    • Name
      fen
    • Ordered locus names
      Mbur_1913

Organism names

Accessions

  • Primary accession
    Q12UT1

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10000613231-338Flap endonuclease 1

Interaction

Subunit

Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-98N-domain
Region116-257I-domain
Region330-338Interaction with PCNA

Sequence similarities

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    338
  • Mass (Da)
    37,762
  • Last updated
    2006-08-22 v1
  • Checksum
    26E1B149772A48F4
MGTDIGDLLLKDTIEIAGLSNKVVAIDAYNTLYQFLSIIRQRDGTPLKDSRGQITSHLSGILYRLTSLIEAGVKPIFVFDGKPPDFKSDTLAKRHEVRESATAKWEDAKAQGLEEEAYKYAQASSKVTREMIDDSVRLLELMGIPYVKAPSEGEAQASYMVQKGDADYIGSQDYDSFLFGAPQVVRNLTITGKRKLPKKNIYVDVKPEVLSLVDSLGELGITRQQLIDIAMCVGTDYNTGLENIGPKRALKLVKEHGDIKVVLKELGKDIEDLDAKRDFFMNPPVTDDYELKWIKPDRAGVIDLLCKKHDFSEERVNKALDRLEANIGGSQSTLDQWF

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000300
EMBL· GenBank· DDBJ
ABE52795.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp