Q12L39 · XNI_SHEDO

Function

function

Has flap endonuclease activity. During DNA replication, flap endonucleases cleave the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment.

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 Mg2+ per subunit. Only one magnesium ion has a direct interaction with the protein, the other interactions are indirect.
K+ (UniProtKB | Rhea| CHEBI:29103 )

Note: Binds 1 K+ per subunit. The potassium ion strongly increases the affinity for DNA.

Features

Showing features for binding site.

125220406080100120140160180200220240
TypeIDPosition(s)Description
Binding site105Mg2+ (UniProtKB | ChEBI)
Binding site172K+ (UniProtKB | ChEBI)
Binding site173K+ (UniProtKB | ChEBI)
Binding site181K+ (UniProtKB | ChEBI)
Binding site183K+ (UniProtKB | ChEBI)
Binding site186K+ (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Molecular Function5'-3' exonuclease activity
Molecular Function5'-flap endonuclease activity
Molecular FunctionDNA binding
Molecular Functionmagnesium ion binding
Molecular Functionpotassium ion binding
Biological ProcessDNA replication, Okazaki fragment processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Flap endonuclease Xni
  • EC number
  • Short names
    FEN

Gene names

    • Name
      xni
    • Synonyms
      ygdG
    • Ordered locus names
      Sden_2558

Organism names

Accessions

  • Primary accession
    Q12L39

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002978741-252Flap endonuclease Xni

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain162-2515'-3' exonuclease
Region185-190Interaction with DNA

Sequence similarities

Belongs to the Xni family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    252
  • Mass (Da)
    28,133
  • Last updated
    2006-08-22 v1
  • Checksum
    55C13824CBC5B037
MNTLLIIDGLNLVRRIHAAQPDENDINNVQQMTLLACTKMLKTHQPSHVIVVWDGDEESWRKKLYSDYKKGRKPMPEALKIALVGIRNTLSEAGIHSLEAESEADDVIATLATKMVENSGAAIIVSTDKGFSQLVKKNIQIWDHFKQLFLDISAHEKKLAVEQYQFLDFIALAGDSGNKIPGIAGIGPKSAADLLNKFRTLANIYNSLDNLGAKQAQKLAEGREMARISYKLGQLQCHMPLEINLKQFRVKK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000302
EMBL· GenBank· DDBJ
ABE55837.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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