Q12320 · GLO4_YEAST

Function

function

Thiolesterase that catalyzes the hydrolysis of S-D-lactoylglutathione to form glutathione and D-lactic acid. Involved in the metabolism of methylglyoxal, a toxic compound for yeast proliferation, by converting methylglyoxal to lactate via S-D-lactoylglutathione by sequential enzyme reactions catalyzed by glyoxalase I and glyoxalase II.

Caution

The enzyme reported here differs from 2 previously reported yeast proteins with glyoxalase II activity both in molecular weight and in the kinetic parameters determined (Ref.5, Ref.6). The lower molecular masses and the much lower specific activities could indicate that these proteins were degradation products of either GLO2 or GLO4. In extracts of a yeast strain lacking both glyoxalase II genes no residual glyoxalase II activity was detected, indicating that there is no further protein with glyoxalase II activity present in yeast cells (PubMed:9261170).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per subunit.

Activity regulation

Inhibited by various thiol compounds such as glutathione and coenzyme A.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
79.3 μM(R)-S-lactoylglutathione
25.6 μM(R)-S-mandeloylglutathione
kcat is 605 sec-1 with (R)-S-lactoylglutathione as substrate and 38.1 sec-1 with (R)-S-mandeloylglutathione as substrate.

pH Dependence

Optimum pH is 6.5-9.

Pathway

Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site69Zn2+ 1 (UniProtKB | ChEBI)
Binding site71Zn2+ 1 (UniProtKB | ChEBI)
Binding site73Zn2+ 2 (UniProtKB | ChEBI)
Binding site74Zn2+ 2 (UniProtKB | ChEBI)
Binding site131Zn2+ 1 (UniProtKB | ChEBI)
Binding site154Zn2+ 1 (UniProtKB | ChEBI)
Binding site154Zn2+ 2 (UniProtKB | ChEBI)
Binding site198Zn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmitochondrial matrix
Cellular Componentmitochondrion
Molecular Functionhydroxyacylglutathione hydrolase activity
Molecular Functionmetal ion binding
Biological Processmethylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Hydroxyacylglutathione hydrolase, mitochondrial
  • EC number
  • Alternative names
    • Glyoxalase II
      (Glx II)

Gene names

    • Name
      GLO4
    • Synonyms
      GER1
    • ORF names
      O2758, OR26.33
    • Ordered locus names
      YOR040W

Organism names

Accessions

  • Primary accession
    Q12320
  • Secondary accessions
    • D6W2A7

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

Type
IDPosition(s)Description
Transit peptide1-10Mitochondrion
ChainPRO_000001228511-285Hydroxyacylglutathione hydrolase, mitochondrial

Proteomic databases

PTM databases

Interaction

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    285
  • Mass (Da)
    32,339
  • Last updated
    1996-11-01 v1
  • Checksum
    09DF2E4B5B9C0E52
MKFLLQQIRNMHVKPIKMRWLTGGVNYSYLLSTEDRRNSWLIDPAEPLEVSPKLSAEEKKSIDAIVNTHHHYDHSGGNLALYSILCQENSGHDIKIIGGSKSSPGVTEVPDNLQQYHLGNLRVTCIRTPCHTKDSICYYIKDLETGEQCIFTGDTLFIAGCGRFFEGTGRDMDMALNQIMLRAVGETNWNKVKIYPGHEYTKGNVSFIRAKIYSDIGQNKEFDALEQYCKSNECTTGHFTLRDELGYNPFMRLDDRAVRLAVGDTAGTYPRSVVMQELRKLKNAM

Mass Spectrometry

Molecular mass is 31,036 Da. Determined by MALDI.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X82893
EMBL· GenBank· DDBJ
CAA58065.1
EMBL· GenBank· DDBJ
Genomic DNA
X87331
EMBL· GenBank· DDBJ
CAA60759.1
EMBL· GenBank· DDBJ
Genomic DNA
Z74948
EMBL· GenBank· DDBJ
CAA99230.1
EMBL· GenBank· DDBJ
Genomic DNA
AY692965
EMBL· GenBank· DDBJ
AAT92984.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006948
EMBL· GenBank· DDBJ
DAA10823.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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