Q12284 · ERV2_YEAST
- ProteinFAD-linked sulfhydryl oxidase ERV2
- GeneERV2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids196 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation in the endoplasmic reticulum lumen in parallel to ERO1.
Miscellaneous
Present with 259 molecules/cell in log phase SD medium.
Catalytic activity
- O2 + 2 R'C(R)SH = H2O2 + R'C(R)S-S(R)CR'
Cofactor
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 78 | FAD (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 83 | FAD (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 86 | FAD (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 127 | FAD (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 150 | FAD (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 153 | FAD (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 157 | FAD (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 162 | FAD (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 174 | FAD (UniProtKB | ChEBI) | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | fungal-type vacuole | |
Cellular Component | fungal-type vacuole membrane | |
Cellular Component | mitochondrion | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | flavin-dependent sulfhydryl oxidase activity | |
Molecular Function | thiol oxidase activity | |
Biological Process | regulation of protein folding in endoplasmic reticulum |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFAD-linked sulfhydryl oxidase ERV2
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ12284
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass type III membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-12 | Cytoplasmic | ||||
Sequence: MKQIVKRSHAIR | ||||||
Transmembrane | 13-35 | Helical; Signal-anchor | ||||
Sequence: IVAALGIIGLWMFFSSNELSIAT | ||||||
Topological domain | 36-196 | Lumenal | ||||
Sequence: PGLIKAKSGIDEVQGAAAEKNDARLKEIEKQTIMPLMGDDKVKKEVGRASWKYFHTLLARFPDEPTPEEREKLHTFIGLYAELYPCGECSYHFVKLIEKYPVQTSSRTAAAMWGCHIHNKVNEYLKKDIYDCATILEDYDCGCSDSDGKRVSLEKEAKQHG |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 121 | Loss of function. | ||||
Sequence: C → A | ||||||
Mutagenesis | 124 | Loss of function. | ||||
Sequence: C → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000001188 | 1-196 | FAD-linked sulfhydryl oxidase ERV2 | |||
Sequence: MKQIVKRSHAIRIVAALGIIGLWMFFSSNELSIATPGLIKAKSGIDEVQGAAAEKNDARLKEIEKQTIMPLMGDDKVKKEVGRASWKYFHTLLARFPDEPTPEEREKLHTFIGLYAELYPCGECSYHFVKLIEKYPVQTSSRTAAAMWGCHIHNKVNEYLKKDIYDCATILEDYDCGCSDSDGKRVSLEKEAKQHG | ||||||
Disulfide bond | 121↔124 | Redox-active | ||||
Sequence: CGEC | ||||||
Disulfide bond | 150↔167 | |||||
Sequence: CHIHNKVNEYLKKDIYDC | ||||||
Disulfide bond | 176↔178 | |||||
Sequence: CGC |
Keywords
- PTM
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 72-174 | ERV/ALR sulfhydryl oxidase | ||||
Sequence: MGDDKVKKEVGRASWKYFHTLLARFPDEPTPEEREKLHTFIGLYAELYPCGECSYHFVKLIEKYPVQTSSRTAAAMWGCHIHNKVNEYLKKDIYDCATILEDY |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length196
- Mass (Da)22,141
- Last updated1997-11-01 v1
- Checksum7FEE76B5F01D3D65
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z71255 EMBL· GenBank· DDBJ | CAA94987.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z68111 EMBL· GenBank· DDBJ | CAA92143.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006949 EMBL· GenBank· DDBJ | DAA11464.1 EMBL· GenBank· DDBJ | Genomic DNA |