Q12211 · PUS1_YEAST

Function

function

Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr) (PubMed:10356324, PubMed:25219674, PubMed:9671058).
Catalyzes pseudouridylation at position 44 in U2 snRNA (PubMed:25219674).
Also catalyzes pseudouridylation of mRNAs (PubMed:25219674, PubMed:31477916).

Miscellaneous

Present with 8130 molecules/cell in log phase SD medium.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
420 nMtRNA(Val)
740 nMtRNA(Ile)
kcat is 0.4 min-1 for tRNA(Val) (PubMed:10356324).
kcat is 0.5 min-1 for tRNA(Ile) (PubMed:10356324).

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site134Nucleophile

GO annotations

AspectTerm
Cellular Componentnucleoplasm
Cellular Componentnucleus
Molecular FunctionmRNA binding
Molecular Functionpseudouridine synthase activity
Molecular FunctionsnRNA pseudouridine synthase activity
Molecular FunctiontRNA pseudouridine synthase activity
Biological ProcessmRNA processing
Biological ProcessmRNA pseudouridine synthesis
Biological ProcesssnRNA pseudouridine synthesis
Biological ProcesstRNA modification
Biological ProcesstRNA pseudouridine synthesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA pseudouridine synthase 1
  • EC number
  • Alternative names
    • tRNA pseudouridylate synthase 1
    • tRNA-uridine isomerase 1

Gene names

    • Name
      PUS1
    • Ordered locus names
      YPL212C

Organism names

Accessions

  • Primary accession
    Q12211
  • Secondary accessions
    • D6W3F8

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000575301-544tRNA pseudouridine synthase 1

Proteomic databases

PTM databases

Interaction

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region1-74Disordered
Region495-544Disordered
Compositional bias504-523Basic and acidic residues
Compositional bias524-538Polar residues

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    544
  • Mass (Da)
    62,143
  • Last updated
    1996-11-01 v1
  • Checksum
    1A7E449601C26EFD
MSEENLRPAYDDQVNEDVYKRGAQSKLTKARKADFDDEKDKKKDNDKHIDKRPKSGPRLDENGNPLPKEPRLPKRKVAVMVGYCGTGYHGMQYNPPNPTIESALFKAFVEAGAISKDNSNDLKKNGFMRAARTDKGVHAGGNLISLKMIIEDPDIKQKINEKLPEGIRVWDIERVNKAFDCRKMCSSRWYEYLLPTYSLIGPKPGSILYRDIEESKTELPGVLDEDLESKEFWEEFKKDANEKFSTEEIEAILAYVPPARDEFDINEELYQKVKKYKQLENAHRRRYRISAAKLAKFRASTSQYLGAHNFHNFTLGKDFKEPSAIRFMKDIKVSDPFVIGDAQTEWISIKIHGQSFMLHQIRKMVSMATLITRCGCPVERISQAYGQQKINIPKAPALGLLLEAPVFEGYNKRLEQFGYKAIDFSKYQDEVDKFKMKHIYDKIYKEEVDENVFNAFFSYIDSFNKVTGAQGEETADKSGPAVQKSIFEFLTAKGIPGLTDAPESNKKIKQRKRMEEEEAASKKAEISSTTQSNEPEVQPEAAAN

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias504-523Basic and acidic residues
Compositional bias524-538Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X80673
EMBL· GenBank· DDBJ
CAA56698.1
EMBL· GenBank· DDBJ
Genomic DNA
Z73568
EMBL· GenBank· DDBJ
CAA97927.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006949
EMBL· GenBank· DDBJ
DAA11224.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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