Q12211 · PUS1_YEAST
- ProteintRNA pseudouridine synthase 1
- GenePUS1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids544 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr) (PubMed:10356324, PubMed:25219674, PubMed:9671058).
Catalyzes pseudouridylation at position 44 in U2 snRNA (PubMed:25219674).
Also catalyzes pseudouridylation of mRNAs (PubMed:25219674, PubMed:31477916).
Catalyzes pseudouridylation at position 44 in U2 snRNA (PubMed:25219674).
Also catalyzes pseudouridylation of mRNAs (PubMed:25219674, PubMed:31477916).
Miscellaneous
Present with 8130 molecules/cell in log phase SD medium.
Catalytic activity
- a uridine in tRNA = a pseudouridine in tRNA
- uridine in snRNA = pseudouridine in snRNA
- a uridine in mRNA = a pseudouridine in mRNA
Cofactor
Note: Binds 1 zinc ion per subunit.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
420 nM | tRNA(Val) | |||||
740 nM | tRNA(Ile) |
kcat is 0.4 min-1 for tRNA(Val) (PubMed:10356324).
kcat is 0.5 min-1 for tRNA(Ile) (PubMed:10356324).
kcat is 0.5 min-1 for tRNA(Ile) (PubMed:10356324).
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 134 | Nucleophile | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Molecular Function | mRNA binding | |
Molecular Function | pseudouridine synthase activity | |
Molecular Function | snRNA pseudouridine synthase activity | |
Molecular Function | tRNA pseudouridine synthase activity | |
Biological Process | mRNA processing | |
Biological Process | mRNA pseudouridine synthesis | |
Biological Process | snRNA pseudouridine synthesis | |
Biological Process | tRNA modification | |
Biological Process | tRNA pseudouridine synthesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA pseudouridine synthase 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ12211
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000057530 | 1-544 | tRNA pseudouridine synthase 1 | |||
Sequence: MSEENLRPAYDDQVNEDVYKRGAQSKLTKARKADFDDEKDKKKDNDKHIDKRPKSGPRLDENGNPLPKEPRLPKRKVAVMVGYCGTGYHGMQYNPPNPTIESALFKAFVEAGAISKDNSNDLKKNGFMRAARTDKGVHAGGNLISLKMIIEDPDIKQKINEKLPEGIRVWDIERVNKAFDCRKMCSSRWYEYLLPTYSLIGPKPGSILYRDIEESKTELPGVLDEDLESKEFWEEFKKDANEKFSTEEIEAILAYVPPARDEFDINEELYQKVKKYKQLENAHRRRYRISAAKLAKFRASTSQYLGAHNFHNFTLGKDFKEPSAIRFMKDIKVSDPFVIGDAQTEWISIKIHGQSFMLHQIRKMVSMATLITRCGCPVERISQAYGQQKINIPKAPALGLLLEAPVFEGYNKRLEQFGYKAIDFSKYQDEVDKFKMKHIYDKIYKEEVDENVFNAFFSYIDSFNKVTGAQGEETADKSGPAVQKSIFEFLTAKGIPGLTDAPESNKKIKQRKRMEEEEAASKKAEISSTTQSNEPEVQPEAAAN |
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-74 | Disordered | ||||
Sequence: MSEENLRPAYDDQVNEDVYKRGAQSKLTKARKADFDDEKDKKKDNDKHIDKRPKSGPRLDENGNPLPKEPRLPK | ||||||
Region | 495-544 | Disordered | ||||
Sequence: IPGLTDAPESNKKIKQRKRMEEEEAASKKAEISSTTQSNEPEVQPEAAAN | ||||||
Compositional bias | 504-523 | Basic and acidic residues | ||||
Sequence: SNKKIKQRKRMEEEEAASKK | ||||||
Compositional bias | 524-538 | Polar residues | ||||
Sequence: AEISSTTQSNEPEVQ |
Sequence similarities
Belongs to the tRNA pseudouridine synthase TruA family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length544
- Mass (Da)62,143
- Last updated1996-11-01 v1
- Checksum1A7E449601C26EFD
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 504-523 | Basic and acidic residues | ||||
Sequence: SNKKIKQRKRMEEEEAASKK | ||||||
Compositional bias | 524-538 | Polar residues | ||||
Sequence: AEISSTTQSNEPEVQ |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X80673 EMBL· GenBank· DDBJ | CAA56698.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z73568 EMBL· GenBank· DDBJ | CAA97927.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006949 EMBL· GenBank· DDBJ | DAA11224.1 EMBL· GenBank· DDBJ | Genomic DNA |