Q12186 · BBP_YEAST

Function

function

Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. 2 commitment complexes, CC1 and CC2, have been defined. CC1 is a basal complex dependent only on the 5'-splice site. CC2 is a complex of lower mobility and is dependent on a branchpoint as well as a 5'-splice site region. This protein is involved in CC2 formation where it binds to the snRNP U1-associated protein PRP40, bridging the U1 snRNP-associated 5'-splice site and the MSL5-associated branch point 3' intron splice site. Involved in nuclear retention of pre-mRNA.

Miscellaneous

Present with 4380 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcommitment complex
Cellular Componentcytosol
Cellular Componentnucleus
Molecular FunctionmRNA binding
Molecular Functionpre-mRNA branch point binding
Molecular Functionzinc ion binding
Biological ProcessmRNA splicing, via spliceosome
Biological Processregulation of mRNA splicing, via spliceosome
Biological Processspliceosomal complex assembly

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Branchpoint-bridging protein
  • Alternative names
    • Mud synthetic-lethal 5 protein
    • Splicing factor 1
    • Zinc finger protein BBP

Gene names

    • Name
      MSL5
    • Synonyms
      BBP, SF1
    • ORF names
      L2949
    • Ordered locus names
      YLR116W

Organism names

Accessions

  • Primary accession
    Q12186
  • Secondary accessions
    • D6VYB4

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis60In MSL5-2; temperature-sensitive; growth defect; when associated with N-72 and S-155.
Mutagenesis72In MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and S-155.
Mutagenesis155In MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and N-72.
Mutagenesis195In MSL5-5; cold-sensitive; growth defect; when associated with V-258.
Mutagenesis230In msl-5; loss of function.
Mutagenesis258In MSL5-5; cold-sensitive; growth defect; when associated with D-195.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 12 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002345641-476Branchpoint-bridging protein
Modified residue93Phosphoserine
Modified residue95Phosphoserine
Modified residue100Phosphotyrosine
Modified residue382Phosphoserine

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Interacts with MUD2 and PRP40. The proline-rich sequence of this protein interacts with the GYF domains of SMY2 and SYH1.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q12186MUD2 P360846EBI-34012, EBI-11612
View interactors in UniProtKB
View CPX-1417 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, domain, zinc finger, compositional bias.

TypeIDPosition(s)Description
Region83-110Disordered
Domain153-219KH
Zinc finger271-288CCHC-type 1
Zinc finger297-314CCHC-type 2
Compositional bias329-366Polar residues
Region329-476Disordered
Compositional bias376-408Polar residues
Compositional bias412-432Pro residues

Sequence similarities

Belongs to the BBP/SF1 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    476
  • Mass (Da)
    53,034
  • Last updated
    1996-11-01 v1
  • Checksum
    F94FF7175345C47D
MSFRRINSRYFENRKGSSMEEKKAKVPPNVNLSLWRKNTVESDVHRFNSLPSKISGALTREQIYSYQVMFRIQEITIKLRTNDFVPPSRKNRSPSPPPVYDAQGKRTNTREQRYRKKLEDERIKLVEIALKTIPYFVPPDDYKRPTKFQDKYYIPVDQYPDVNFVGLLLGPRGRTLRKLQEDSNCKIAIRGRGSVKEGKNASDLPPGAMNFEDPLHCLIIADSEDKIQKGIKVCQNIVIKAVTSPEGQNDLKRGQLRELAELNGTLREDNRPCPICGLKDHKRYDCPNRKIPNIQGIVCKICGQTGHFSRDCNSSSQRMSRFDRNATVNNSAPIQSNDVHYNSNTHPIQAPKRSRYDNNSTEPPLKFPASSRYAPSPSPPASHISRQAQNVTPTPPPGLTSSSFSSGVPGIAPPPLQSPPESEQPKFSLPPPPGMTTVQSSIAPPPGLSGPPGFSNNMGNDINKPTPPGLQGPPGL

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias329-366Polar residues
Compositional bias376-408Polar residues
Compositional bias412-432Pro residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X89514
EMBL· GenBank· DDBJ
CAA61695.1
EMBL· GenBank· DDBJ
Genomic DNA
U53877
EMBL· GenBank· DDBJ
AAB82363.1
EMBL· GenBank· DDBJ
Genomic DNA
Z73288
EMBL· GenBank· DDBJ
CAA97683.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006945
EMBL· GenBank· DDBJ
DAA09430.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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