Q12093 · MTU1_YEAST

Function

function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.

Miscellaneous

Present with 1510 molecules/cell in log phase SD medium.
During the reaction, ATP is used to activate the C2 atom of U34 by adenylation. After this, the persulfide sulfur on the catalytic cysteine is transferred to the C2 atom of the wobble base (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as a nucleophile towards the activated C2 atom on U34. Subsequently, a transient disulfide bond is formed between the two active site cysteine residues (By similarity).

Caution

Was originally thought to be a 5-methylaminomethyl-2-methyltransferase involved in tRNA modification.

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site32-39ATP (UniProtKB | ChEBI)
Binding site58ATP (UniProtKB | ChEBI)
Active site127Nucleophile
Binding site154ATP (UniProtKB | ChEBI)
Site155Interaction with tRNA
Active site229Cysteine persulfide intermediate
Site281Interaction with tRNA
Site391Interaction with tRNA

GO annotations

AspectTerm
Cellular Componentmitochondrial matrix
Cellular Componentmitochondrion
Molecular FunctionATP binding
Molecular Functionsulfurtransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-5-taurinomethyluridine 2-sulfurtransferase
Biological Processmitochondrial tRNA wobble position uridine thiolation
Biological ProcesstRNA modification
Biological ProcesstRNA wobble position uridine thiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Mitochondrial tRNA-specific 2-thiouridylase 1
  • EC number
  • Alternative names
    • Mitochondrial translation optimization protein 2
    • Synthetic lethal with MSS4 3

Gene names

    • Name
      SLM3
    • Synonyms
      MTO2, MTU1
    • ORF names
      D2761
    • Ordered locus names
      YDL033C

Organism names

Accessions

  • Primary accession
    Q12093
  • Secondary accessions
    • D6VRW0
    • Q6IUF4

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis38Loss of activity.

Variants

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The viewer provides 9 variants from UniProt as well as other sources including ClinVar and dbSNP.

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PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00001217111-417Mitochondrial tRNA-specific 2-thiouridylase 1
Disulfide bond127↔229Alternate

Keywords

Proteomic databases

PTM databases

Interaction

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q12093HSP60 P198822EBI-19558, EBI-8586

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region122-124Interaction with target base in tRNA
Region179-181Interaction with tRNA
Region354-355Interaction with tRNA

Sequence similarities

Belongs to the MnmA/TRMU family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    417
  • Mass (Da)
    47,049
  • Last updated
    1996-11-01 v1
  • Checksum
    15BF63FD9A094890
MLARYLNLIGRRSASPYRPQRLPAKFDNVIVAMSSGVDSSVAAALFAGEFPNTRGVYMQNWSESQSLDDPGKEPCYERDWRDVNRVAKHLNIRVDKVNFEQDYWIDVFEPMLRGYSEGSTPNPDIGCNKFVKFGKLREWLDEKYGTGNYWLVTGHYARVMQEMNGKGLFHLLRSIYRPKDQSYYLSQINSTVLSSLLLPIGHLTKPEVRDLAKYAGLPTAEKPDSQGICFVNNSQHGKFKNFLKHYLPSSPGDIITVDPQSGAKTTWGRHDGLWSYTIGQKVGISMPQADPNYQGTWFVSEKLRDTNEILIVRGRDNPALYSDTMRIENFSSLGPREDTINAFQNTGALTLQFRSLQVPVQIKSCKLNRSADNLDITIHLASKQRAITPGQSCCLYIDDRVLGSGPISHVNNNDTHA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY624369
EMBL· GenBank· DDBJ
AAT44736.1
EMBL· GenBank· DDBJ
Genomic DNA
Z71781
EMBL· GenBank· DDBJ
CAA96456.1
EMBL· GenBank· DDBJ
Genomic DNA
Z74081
EMBL· GenBank· DDBJ
CAA98591.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006938
EMBL· GenBank· DDBJ
DAA11820.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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