Q12093 · MTU1_YEAST
- ProteinMitochondrial tRNA-specific 2-thiouridylase 1
- GeneSLM3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids417 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.
Miscellaneous
Present with 1510 molecules/cell in log phase SD medium.
During the reaction, ATP is used to activate the C2 atom of U34 by adenylation. After this, the persulfide sulfur on the catalytic cysteine is transferred to the C2 atom of the wobble base (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as a nucleophile towards the activated C2 atom on U34. Subsequently, a transient disulfide bond is formed between the two active site cysteine residues (By similarity).
Catalytic activity
- 5-taurinomethyluridine34 in tRNA + AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] = 5-taurinomethyl-2-thiouridine34 in tRNA + A + AMP + diphosphate + H+ + L-cysteinyl-[protein]
RHEA-COMP:11732 CHEBI:87172 Position: 34+ CHEBI:17499 + CHEBI:30616 + RHEA-COMP:11726 = RHEA-COMP:11733 + CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378 + RHEA-COMP:10131
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 32-39 | ATP (UniProtKB | ChEBI) | ||||
Sequence: AMSSGVDS | ||||||
Binding site | 58 | ATP (UniProtKB | ChEBI) | ||||
Sequence: M | ||||||
Active site | 127 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 154 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Site | 155 | Interaction with tRNA | ||||
Sequence: H | ||||||
Active site | 229 | Cysteine persulfide intermediate | ||||
Sequence: C | ||||||
Site | 281 | Interaction with tRNA | ||||
Sequence: K | ||||||
Site | 391 | Interaction with tRNA | ||||
Sequence: Q |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial matrix | |
Cellular Component | mitochondrion | |
Molecular Function | ATP binding | |
Molecular Function | sulfurtransferase activity | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-5-taurinomethyluridine 2-sulfurtransferase | |
Biological Process | mitochondrial tRNA wobble position uridine thiolation | |
Biological Process | tRNA modification | |
Biological Process | tRNA wobble position uridine thiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMitochondrial tRNA-specific 2-thiouridylase 1
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ12093
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 38 | Loss of activity. | ||||
Sequence: D → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 9 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000121711 | 1-417 | Mitochondrial tRNA-specific 2-thiouridylase 1 | |||
Sequence: MLARYLNLIGRRSASPYRPQRLPAKFDNVIVAMSSGVDSSVAAALFAGEFPNTRGVYMQNWSESQSLDDPGKEPCYERDWRDVNRVAKHLNIRVDKVNFEQDYWIDVFEPMLRGYSEGSTPNPDIGCNKFVKFGKLREWLDEKYGTGNYWLVTGHYARVMQEMNGKGLFHLLRSIYRPKDQSYYLSQINSTVLSSLLLPIGHLTKPEVRDLAKYAGLPTAEKPDSQGICFVNNSQHGKFKNFLKHYLPSSPGDIITVDPQSGAKTTWGRHDGLWSYTIGQKVGISMPQADPNYQGTWFVSEKLRDTNEILIVRGRDNPALYSDTMRIENFSSLGPREDTINAFQNTGALTLQFRSLQVPVQIKSCKLNRSADNLDITIHLASKQRAITPGQSCCLYIDDRVLGSGPISHVNNNDTHA | ||||||
Disulfide bond | 127↔229 | Alternate | ||||
Sequence: CNKFVKFGKLREWLDEKYGTGNYWLVTGHYARVMQEMNGKGLFHLLRSIYRPKDQSYYLSQINSTVLSSLLLPIGHLTKPEVRDLAKYAGLPTAEKPDSQGIC |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q12093 | HSP60 P19882 | 2 | EBI-19558, EBI-8586 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 122-124 | Interaction with target base in tRNA | ||||
Sequence: NPD | ||||||
Region | 179-181 | Interaction with tRNA | ||||
Sequence: KDQ | ||||||
Region | 354-355 | Interaction with tRNA | ||||
Sequence: RS |
Sequence similarities
Belongs to the MnmA/TRMU family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length417
- Mass (Da)47,049
- Last updated1996-11-01 v1
- Checksum15BF63FD9A094890
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY624369 EMBL· GenBank· DDBJ | AAT44736.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z71781 EMBL· GenBank· DDBJ | CAA96456.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z74081 EMBL· GenBank· DDBJ | CAA98591.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006938 EMBL· GenBank· DDBJ | DAA11820.1 EMBL· GenBank· DDBJ | Genomic DNA |