Q11068 · MGAT1_CAEEL
- ProteinPutative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
- Genegly-13
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids449 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans (By similarity).
Catalytic activity
- N4-(alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + UDP-N-acetyl-alpha-D-glucosamine = H+ + N4-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP
Cofactor
Note: The cofactor is mostly bound to the substrate.
Pathway
Protein modification; protein glycosylation.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi apparatus | |
Cellular Component | Golgi medial cisterna | |
Cellular Component | Golgi membrane | |
Cellular Component | perinuclear region of cytoplasm | |
Molecular Function | alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | protein N-linked glycosylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namePutative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ11068
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-6 | Cytoplasmic | ||||
Sequence: MHAVTK | ||||||
Transmembrane | 7-23 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: IFIIFIFVFILWTLYVE | ||||||
Topological domain | 24-449 | Lumenal | ||||
Sequence: NDITNRTRNTDNIDDLLESANRLERLLKFEAKKIAALAEDVHKIRANRKGKHVIMEEMVSQDLKQWKDPIPVLVFSCNRAMAVRDHVEKLIRYRPSQEKFPIIVTQDCDNENVKNEVKKFGDKVEYIKHLAGDKANITIPPSHRQYTAYYRIARHYKLALNHVFVDKGYSSVIITEDDLDISPDFFSYFSSTRYLLENDEKLWCVTAWNDNGKQENIDMTAASTLYRSDFFAGLGWMMSSKTWHELEPIWPVGFWDDWMRDPARRKDRQCIRPEISRTGMMSYGKEGASKGQFFSKHLAKIKVNDKYINFGKIDLDYLLPANFAKKTNLEVMKEAVELSIDNVASFVLSSENKGKSVRVMYDGNIDYIRKADKLHIMHDFKAGVPRTAYDGIVTCFINGIRIYLVPDRTKVSAYNPDWSVPPSFGE |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000191388 | 1-449 | Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase | |||
Sequence: MHAVTKIFIIFIFVFILWTLYVENDITNRTRNTDNIDDLLESANRLERLLKFEAKKIAALAEDVHKIRANRKGKHVIMEEMVSQDLKQWKDPIPVLVFSCNRAMAVRDHVEKLIRYRPSQEKFPIIVTQDCDNENVKNEVKKFGDKVEYIKHLAGDKANITIPPSHRQYTAYYRIARHYKLALNHVFVDKGYSSVIITEDDLDISPDFFSYFSSTRYLLENDEKLWCVTAWNDNGKQENIDMTAASTLYRSDFFAGLGWMMSSKTWHELEPIWPVGFWDDWMRDPARRKDRQCIRPEISRTGMMSYGKEGASKGQFFSKHLAKIKVNDKYINFGKIDLDYLLPANFAKKTNLEVMKEAVELSIDNVASFVLSSENKGKSVRVMYDGNIDYIRKADKLHIMHDFKAGVPRTAYDGIVTCFINGIRIYLVPDRTKVSAYNPDWSVPPSFGE | ||||||
Glycosylation | 28 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 100↔131 | |||||
Sequence: CNRAMAVRDHVEKLIRYRPSQEKFPIIVTQDC | ||||||
Glycosylation | 159 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 227↔293 | |||||
Sequence: CVTAWNDNGKQENIDMTAASTLYRSDFFAGLGWMMSSKTWHELEPIWPVGFWDDWMRDPARRKDRQC |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Structure
Sequence
- Sequence statusComplete
- Length449
- Mass (Da)52,151
- Last updated2001-08-14 v2
- Checksum8448210B2A78FC88
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF082010 EMBL· GenBank· DDBJ | AAD03022.1 EMBL· GenBank· DDBJ | mRNA | ||
FO080200 EMBL· GenBank· DDBJ | CCD61925.1 EMBL· GenBank· DDBJ | Genomic DNA |