Q0ZPV7 · CXE1_ACTER
- ProteinCarboxylesterase 1
- GeneCXE1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids335 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Carboxylesterase acting on esters with varying acyl chain length.
Catalytic activity
- a carboxylic ester + H2O = a carboxylate + an alcohol + H+
Activity regulation
Is inhibited by the organophosphates paraoxon and dimethylchlorophosphate (DMCP).
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
33.3 μM | 4-methylumbelliferyl acetate | |||||
16.7 μM | 4-methylumbelliferyl butyrate | |||||
19.1 μM | 4-methylumbelliferyl heptanoate | |||||
6.34 μM | 4-methylumbelliferyl octanoate | |||||
0.117 μM | 4-methylumbelliferyl laureate | |||||
0.024 μM | 4-methylumbelliferyl palmitate |
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 92-93 | paraoxon (UniProtKB | ChEBI); inhibitor | ||||
Sequence: GG | ||||||
Active site | 169 | |||||
Sequence: S | ||||||
Binding site | 169 | paraoxon (UniProtKB | ChEBI); inhibitor; covalent | ||||
Sequence: S | ||||||
Binding site | 170 | paraoxon (UniProtKB | ChEBI); inhibitor | ||||
Sequence: A | ||||||
Active site | 276 | |||||
Sequence: D | ||||||
Active site | 306 | |||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carboxylesterase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCarboxylesterase 1
- EC number
- Short namesAeCXE1
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > Ericales > Actinidiaceae > Actinidia
Accessions
- Primary accessionQ0ZPV7
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000403482 | 1-335 | Carboxylesterase 1 | |||
Sequence: MSNDHLETTGSSDPNTNLLKYLPIVLNPDRTITRPIQIPSTAASPDPTSSSPVLTKDLALNPLHNTFVRLFLPRHALYNSAKLPLVVYFHGGGFILFSAASTIFHDFCCEMAVHAGVVIASVDYRLAPEHRLPAAYDDAMEALQWIKDSRDEWLTNFADFSNCFIMGESAGGNIAYHAGLRAAAVADELLPLKIKGLVLDEPGFGGSKRTGSELRLANDSRLPTFVLDLIWELSLPMGADRDHEYCNPTAESEPLYSFDKIRSLGWRVMVVGCHGDPMIDRQMELAERLEKKGVDVVAQFDVGGYHAVKLEDPEKAKQFFVILKKFVVDSCTTKL |
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 90-92 | Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole | ||||
Sequence: HGG |
Sequence similarities
Belongs to the 'GDXG' lipolytic enzyme family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length335
- Mass (Da)37,052
- Last updated2006-08-22 v1
- ChecksumCA859F19267B8434
Keywords
- Technical term