Q0Z971 · Q0Z971_ICMV

Function

function

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the + strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Divalent metal cations, possibly Mg2+ or Mn2+.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

121320406080100120140160180200
TypeIDPosition(s)Description
Binding site14a divalent metal cation (UniProtKB | ChEBI)
Binding site22a divalent metal cation (UniProtKB | ChEBI)
Binding site24a divalent metal cation (UniProtKB | ChEBI)
Active site68For DNA cleavage activity
Binding site72a divalent metal cation (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell nucleus
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular Functionendodeoxyribonuclease activity, producing 5'-phosphomonoesters
Molecular Functionhelicase activity
Molecular Functionmetal ion binding
Molecular Functionnucleotidyltransferase activity
Molecular Functionstructural molecule activity
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Replication-associated protein
  • EC number
  • Short names
    Rep

Organism names

Accessions

  • Primary accession
    Q0Z971

Subcellular Location

Keywords

PTM/Processing

Keywords

Interaction

Subunit

Homooligomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-81CRESS-DNA virus Rep endonuclease

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similarities

Belongs to the geminiviridae Rep protein family.

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    213
  • Mass (Da)
    23,991
  • Last updated
    2006-08-22 v1
  • Checksum
    32428EEE57B69EF5
QTPTNTKFIKICRELHENGEPHLHVLIQFEGKYKCQNQRFFDLVSPSRSAHFHPNIQGAKSSSDVKSYIDKDGDTLEWGTFQIDGRSARGGQQSANDAYAAALNSGSKSAALTILKELAPRDYIRDFHHINSNLDRIFTTPPPPYQNPFPLSSFDRVPEELDEWFHENVMDAAARPLRPKSIVIEGDSRTGKTMWARALGSHNYLCGHLDLSP

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1
Non-terminal residue213

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DQ658178
EMBL· GenBank· DDBJ
ABG54448.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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