Q0VGT4 · ZGRF1_MOUSE
- Protein5'-3' DNA helicase ZGRF1
- GeneZgrf1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1863 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
5'-3' DNA helicase which is recruited to sites of DNA damage and promotes repair of replication-blocking DNA lesions through stimulation of homologous recombination (HR). Promotes HR by directly stimulating RAD51-mediated strand exchange activity. Not required to load RAD51 at sites of DNA damage but promotes recombinational repair after RAD51 recruitment. Also promotes HR by positively regulating EXO1-mediated DNA end resection of DNA double-strand breaks. Required for recruitment of replication protein RPA2 to DNA damage sites. Promotes the initiation of the G2/M checkpoint but not its maintenance. Catalyzes Holliday junction branch migration and dissociation of D-loops and DNA flaps.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
- Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction. This creates two antiparallel DNA single strands (ssDNA). The leading ssDNA polymer is the template for DNA polymerase III holoenzyme which synthesizes a continuous strand.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | 5'-3' DNA helicase activity | |
Molecular Function | ATP binding | |
Molecular Function | hydrolase activity | |
Molecular Function | isomerase activity | |
Molecular Function | RNA binding | |
Molecular Function | zinc ion binding | |
Biological Process | recombinational repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5'-3' DNA helicase ZGRF1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ0VGT4
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000286627 | 1-1863 | 5'-3' DNA helicase ZGRF1 | |||
Sequence: MECQEFIVLYTHQKMKKSKVWQDGVLKITHLGNKAILYDDKGACLESLFLKCLEVKPGDDLESERYLITVEEAKAVGSRAVEPDGSREALESGSRTLVSSSRSLGCQPSGLKRKATGFQRPYKMPKKVTITENSEPAASLGDENPGPPGPRLLPTFSSTLPLFPTVGQKDLTPVSTDNQSPITFSNRERSDTPLSLPSSYFKINTNTLGKEDKLCFPVSSETKHSDSLLASEPMRRNGLDSHCPGVSQNVRSKAQILALLKSSSTNRKDLHGEIPGHFPKIEPQGCLNIISKPEEDYAETQSIGNLRCEQQSENPTRTTSRWARYLPSQRSPPCSAADENDTEDKPEAQEDVNIFNLSELLQKKSELFETCSKKGELHSEDKPVDNTCQYWNQEDNLAPSFCKNSSVLVSCSSKENASLLSESDIQYSSKVPVNQHEKVWTREGSQGADADAALEPEYRPVSPLPEIGHKQTEVEASLSTSSRISDDIADMGSKSNADREDLKTVHKAVQPFLEVSFNLSNFETSDTDEASQEDSRLSQDSERWEKEAVLTDDSCVQKSCEDIGCREIVGKLPLLSSTDDEPKEALPADGTLLSEFCDRTCVGFNSGPHGDVKTGKALEGQCHSDTGSSLDSSLEWSEDVAGDSREDASQSIQGNAINCGGVSPSKKPRGVNRSLYSPYLLTAVTDPAPENSDLLSEARKSPAIEVSRALLECPCEHRASQQPVEFQGHQVKGSATSGVMVRGHSLQRGCTQFPDSIDYENFLTDTCVWTPGLPSTYGQTDFLQVISPEQKIPALSPAPTFSFNTRNEDTVLELSEESLKTRTLPGLKTIGFQDSKNLQRFPFLSGASAPFATLPADDGPAVLDPCSFMIDDDAREPSGSSMLNLCEESGLSFDLGLEGQGGTPGGVSLLPKSSTQSKWLKYQNPPQCNSTAPNRLASEVTEGLFAEAVSGLHFSHTSESESSVDPVRLQMIKGLLHQQQQDLVSRKQAFSLTLNQTCKTQEHETVLGSSASKNCRAKDLQEINNSDLCFPNGQKIISAYLPQRQVHIPAVFQSPAHYRQVFTASIIEHLNILLFELAQRLYKALSKVDISFYTSSKGETMRSGKNNSPSCHHNQPAKLVMVKKEGPNKGRLFYTCDKSKDNQCKFFKWLEEVTPGQLPQNTSQSTMVFNDIKSIGSYLRSQKVPVYEECQLLLRRGFDFQRKQCGKLKKLTTVNPDFYSETKSKIYLKLSRRESSSVYSKGDLWVISKTLDFELDTFIACSAFFGPSSVNEVELLPLKGYFPSNWPTNITVHALLVCNASTELTTLQNIQDYFNPAALPLMPYLLAMSQSATVSSKNISKRKFIPPAITSIKTKTELHLGATLQLARELINVHRLNKDQATALIQIAQMMASQGSDEDALEPFGHSLPITVIHGVFGAGKSYLLAVVILFLVELFEKCDSGTVGNARPWKVLVSSSTNVAVDRVLLGLLSLGFEKFIRVGSVRKIAKPVLPYSLHAGSDNESEQLKELNALLKEELTPIERVYVRKSIEQHKLGTNRVLLKQVRVVGVTCAACAFPCLNDLKFPVVVLDECSQMTEPASLLPIARFQCEKLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPVLLRTQYRCHPAISAIANDLFYEGSLVNGISERERSPVLEWLPTLCFYNVTGAEQVERENSFVNVAEATFTLKLIQSLMASGVESCMIGVITLYKSQMYKICNLLSAVDVGHPDVKAVQVSTVDAFQGAEKEITILSCVRTRQVGFIDSEKRMNVALTRGRRHLLIVGSLSCLRKNRLWGRVIQHCEGREDGLQHASQCEPQLDHLLKDYLEKQAEEKQKKKEKEKSKDKSH | ||||||
Modified residue | 331 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 445 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Structure
Family & Domains
Features
Showing features for region, compositional bias, zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 78-110 | Disordered | ||||
Sequence: SRAVEPDGSREALESGSRTLVSSSRSLGCQPSG | ||||||
Compositional bias | 92-107 | Polar residues | ||||
Sequence: SGSRTLVSSSRSLGCQ | ||||||
Region | 132-196 | Disordered | ||||
Sequence: ENSEPAASLGDENPGPPGPRLLPTFSSTLPLFPTVGQKDLTPVSTDNQSPITFSNRERSDTPLSL | ||||||
Compositional bias | 158-196 | Polar residues | ||||
Sequence: STLPLFPTVGQKDLTPVSTDNQSPITFSNRERSDTPLSL | ||||||
Region | 300-349 | Disordered | ||||
Sequence: TQSIGNLRCEQQSENPTRTTSRWARYLPSQRSPPCSAADENDTEDKPEAQ | ||||||
Compositional bias | 303-326 | Polar residues | ||||
Sequence: IGNLRCEQQSENPTRTTSRWARYL | ||||||
Region | 460-496 | Disordered | ||||
Sequence: PVSPLPEIGHKQTEVEASLSTSSRISDDIADMGSKSN | ||||||
Compositional bias | 473-489 | Polar residues | ||||
Sequence: EVEASLSTSSRISDDIA | ||||||
Region | 524-545 | Disordered | ||||
Sequence: TSDTDEASQEDSRLSQDSERWE | ||||||
Compositional bias | 529-545 | Basic and acidic residues | ||||
Sequence: EASQEDSRLSQDSERWE | ||||||
Region | 610-664 | Disordered | ||||
Sequence: GDVKTGKALEGQCHSDTGSSLDSSLEWSEDVAGDSREDASQSIQGNAINCGGVSP | ||||||
Compositional bias | 621-635 | Polar residues | ||||
Sequence: QCHSDTGSSLDSSLE | ||||||
Compositional bias | 646-660 | Polar residues | ||||
Sequence: EDASQSIQGNAINCG | ||||||
Zinc finger | 1111-1153 | GRF-type | ||||
Sequence: CHHNQPAKLVMVKKEGPNKGRLFYTCDKSKDNQCKFFKWLEEV |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q0VGT4-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length1,863
- Mass (Da)205,737
- Last updated2013-10-16 v2
- Checksum14D5C95C1FB6727F
Q0VGT4-2
- Name2
- Differences from canonical
- 698-856: ARKSPAIEVSRALLECPCEHRASQQPVEFQGHQVKGSATSGVMVRGHSLQRGCTQFPDSIDYENFLTDTCVWTPGLPSTYGQTDFLQVISPEQKIPALSPAPTFSFNTRNEDTVLELSEESLKTRTLPGLKTIGFQDSKNLQRFPFLSGASAPFATLPA → GTQLFILQSHRDRNDEQVLSPISSSDISGQLLSTTQDHFECHELEESSTQISSPLFYPMGIKHPIYRDSEAYISESKELGRIRSLPCDHFEVETTQRNQQSWATSKSSSELSEVINNMSLLKSQSEHSTALGHLTILKKKHAFPHEVPHSRDPDVSPKG
- 857-1863: Missing
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0G2JGH8 | A0A0G2JGH8_MOUSE | Zgrf1 | 662 | ||
A0A0G2JGJ2 | A0A0G2JGJ2_MOUSE | Zgrf1 | 785 |
Features
Showing features for compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 92-107 | Polar residues | ||||
Sequence: SGSRTLVSSSRSLGCQ | ||||||
Compositional bias | 158-196 | Polar residues | ||||
Sequence: STLPLFPTVGQKDLTPVSTDNQSPITFSNRERSDTPLSL | ||||||
Compositional bias | 303-326 | Polar residues | ||||
Sequence: IGNLRCEQQSENPTRTTSRWARYL | ||||||
Compositional bias | 473-489 | Polar residues | ||||
Sequence: EVEASLSTSSRISDDIA | ||||||
Compositional bias | 529-545 | Basic and acidic residues | ||||
Sequence: EASQEDSRLSQDSERWE | ||||||
Compositional bias | 621-635 | Polar residues | ||||
Sequence: QCHSDTGSSLDSSLE | ||||||
Compositional bias | 646-660 | Polar residues | ||||
Sequence: EDASQSIQGNAINCG | ||||||
Alternative sequence | VSP_053245 | 698-856 | in isoform 2 | |||
Sequence: ARKSPAIEVSRALLECPCEHRASQQPVEFQGHQVKGSATSGVMVRGHSLQRGCTQFPDSIDYENFLTDTCVWTPGLPSTYGQTDFLQVISPEQKIPALSPAPTFSFNTRNEDTVLELSEESLKTRTLPGLKTIGFQDSKNLQRFPFLSGASAPFATLPA → GTQLFILQSHRDRNDEQVLSPISSSDISGQLLSTTQDHFECHELEESSTQISSPLFYPMGIKHPIYRDSEAYISESKELGRIRSLPCDHFEVETTQRNQQSWATSKSSSELSEVINNMSLLKSQSEHSTALGHLTILKKKHAFPHEVPHSRDPDVSPKG | ||||||
Alternative sequence | VSP_053246 | 857-1863 | in isoform 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC113952 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC158308 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC085187 EMBL· GenBank· DDBJ | AAH85187.1 EMBL· GenBank· DDBJ | mRNA |