Q0U5F2 · KU70_PHANO
- ProteinATP-dependent DNA helicase II subunit 1
- GeneKU70
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids652 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching (By similarity).
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome, telomeric region | |
Cellular Component | Ku70:Ku80 complex | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | damaged DNA binding | |
Molecular Function | DNA helicase activity | |
Molecular Function | telomeric DNA binding | |
Biological Process | DNA recombination | |
Biological Process | double-strand break repair via nonhomologous end joining | |
Biological Process | telomere maintenance |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent DNA helicase II subunit 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Dothideomycetes > Pleosporomycetidae > Pleosporales > Pleosporineae > Phaeosphaeriaceae > Parastagonospora
Accessions
- Primary accessionQ0U5F2
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000278346 | 1-652 | ATP-dependent DNA helicase II subunit 1 | |||
Sequence: MADTQDNRPGEEEDDDEEIDESAYKTMKDAVLFAIDVSPSMLERPPKTEDKKADRDSPTSAALKCAYQLMQQRIISNPNDMMGILLFGTEKTDLKDGDSTFQHCYLLADLDVPSAQDVKRLRDLVEDEEEAEQILKPAKDGASIATVLFCANQIFTTKAPNFSSRRLFLVTDNDYPVNVKADKDTAVTRARDLYDLGCTIDLFPISQPDQTFDRSRFYDDLVYPTSPSDPDAPIAVATTTKVAKSGEGITLLKQLISSINSKATPRRALFSLPLELGPDLRIGVKGYILIKRQEHAKSCYVWVGGDKPQIVSSSTSHMADDTARVVEKTELRKAYKFGGDAITFTPDEIIKIRQAFGDPIIRIIGFKPISCLPIWTNTNKATFIYPSEADFIGSTRVFSALQQKLLKSKKMGLVWFIARRNAAPILSALIPAEEQTNEDGEQAMPPGLWLVPLPWADDIRQFPSPAADVLKTTDALTDKMRIIIEQLQLPKGVYDPAKYPNPDLQWFYRILQAMALEEELPEKPDDKTMPKFRQIDKRCGEYITEYGAEFEAAFAQLAVSTFPHRGKRASADPGDDKPAPKRVKKEPKVKEEGEDDEGLTDEQMATVNNKGQISKQTVAVLKAWLSQRGESTSGKKADLVERVQGYLEGKGL |
Interaction
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-22 | Disordered | ||||
Sequence: MADTQDNRPGEEEDDDEEIDES | ||||||
Region | 38-59 | Disordered | ||||
Sequence: SPSMLERPPKTEDKKADRDSPT | ||||||
Compositional bias | 43-58 | Basic and acidic residues | ||||
Sequence: ERPPKTEDKKADRDSP | ||||||
Domain | 274-486 | Ku | ||||
Sequence: LELGPDLRIGVKGYILIKRQEHAKSCYVWVGGDKPQIVSSSTSHMADDTARVVEKTELRKAYKFGGDAITFTPDEIIKIRQAFGDPIIRIIGFKPISCLPIWTNTNKATFIYPSEADFIGSTRVFSALQQKLLKSKKMGLVWFIARRNAAPILSALIPAEEQTNEDGEQAMPPGLWLVPLPWADDIRQFPSPAADVLKTTDALTDKMRIIIEQ | ||||||
Region | 565-612 | Disordered | ||||
Sequence: RGKRASADPGDDKPAPKRVKKEPKVKEEGEDDEGLTDEQMATVNNKGQ | ||||||
Compositional bias | 568-594 | Basic and acidic residues | ||||
Sequence: RASADPGDDKPAPKRVKKEPKVKEEGE | ||||||
Domain | 613-647 | SAP | ||||
Sequence: ISKQTVAVLKAWLSQRGESTSGKKADLVERVQGYL |
Sequence similarities
Belongs to the ku70 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length652
- Mass (Da)72,705
- Last updated2006-09-05 v1
- ChecksumB4062F9323EB5179
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 43-58 | Basic and acidic residues | ||||
Sequence: ERPPKTEDKKADRDSP | ||||||
Compositional bias | 568-594 | Basic and acidic residues | ||||
Sequence: RASADPGDDKPAPKRVKKEPKVKEEGE |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CH445348 EMBL· GenBank· DDBJ | EAT79812.2 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. |