Q0TAL3 · UBID_ECOL5
- Protein3-octaprenyl-4-hydroxybenzoate carboxy-lyase
- GeneubiD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids498 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the decarboxylation of 3-octaprenyl-4-hydroxy benzoate to 2-octaprenylphenol, an intermediate step in ubiquinone biosynthesis.
Catalytic activity
- a 4-hydroxy-3-all-trans-polyprenylbenzoate + H+ = a 2-all-trans-polyprenylphenol + CO2
a 4-hydroxy-3-all-trans-polyprenylbenzoate RHEA-COMP:9514 + CHEBI:15378 = a 2-all-trans-polyprenylphenol RHEA-COMP:9516 + CHEBI:16526
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 prenylated FMN (prenyl-FMN) per subunit.
Pathway
Cofactor biosynthesis; ubiquinone biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 176 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 179-181 | prenyl-FMN (UniProtKB | ChEBI) | ||||
Sequence: IYR | ||||||
Binding site | 193-195 | prenyl-FMN (UniProtKB | ChEBI) | ||||
Sequence: RWL | ||||||
Binding site | 198-199 | prenyl-FMN (UniProtKB | ChEBI) | ||||
Sequence: RG | ||||||
Binding site | 242 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 291 | Proton donor | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | 3-octaprenyl-4-hydroxybenzoate carboxy-lyase activity | |
Molecular Function | metal ion binding | |
Biological Process | ubiquinone biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3-octaprenyl-4-hydroxybenzoate carboxy-lyase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionQ0TAL3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000267665 | 1-498 | 3-octaprenyl-4-hydroxybenzoate carboxy-lyase | |||
Sequence: MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIWLDRTLRAGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPKGFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLITWGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVSVALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGYIEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGVALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQFMYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSKMGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA |
Interaction
Subunit
Homohexamer.
Structure
Sequence
- Sequence statusComplete
- Length498
- Mass (Da)55,832
- Last updated2006-09-05 v1
- Checksum299482D865544121
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000247 EMBL· GenBank· DDBJ | ABG72016.1 EMBL· GenBank· DDBJ | Genomic DNA |