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Q0S4D7 · CASI_RHOJR

Function

function

Involved in cholate catabolism (PubMed:23024343, PubMed:24244004).
Catalyzes the ATP-dependent formation of CoA thioesters of steroids with isopropanoyl side chains, likely occurring as degradation intermediates (PubMed:23024343, PubMed:24244004).
Can use 4-BNC, HSBNC and HIDP as substrate (PubMed:23024343, PubMed:24244004).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
75 μM4-BNC
500 μMHSBNC
190 μMHIDP
kcat is 18 sec-1 with 4-BNC as substrate. kcat is 1.4 sec-1 with HSBNC as substrate. kcat is 60 sec-1 with HIDP as substrate.

Pathway

Steroid metabolism.

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functionlong-chain fatty acid transmembrane transporter activity
Molecular Functionlong-chain fatty acid-CoA ligase activity
Biological Processsteroid metabolic process

Keywords

Enzyme and pathway databases

Chemistry

Names & Taxonomy

Protein names

  • Recommended name
    Steroid-22-oyl-CoA synthetase
  • EC number
  • Alternative names
    • Steroid-CoA synthetase

Gene names

    • Name
      casI
    • Ordered locus names
      RHA1_ro05822

Organism names

Accessions

  • Primary accession
    Q0S4D7

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004521021-555Steroid-22-oyl-CoA synthetase

Expression

Induction

Up-regulated on cholate.

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    555
  • Mass (Da)
    61,164
  • Last updated
    2006-09-05 v1
  • MD5 Checksum
    3C95E4E2257932F2706B73DB22D47256
MELERTTIARMLFDRLGDDRLGVRTREQDWTWDEVVRESAARGAVASSLRRDGPFHVGVLLENTPEFLFWLGGAALAGAAVVGVNPTRRGAELEAEIRYVDCQLIVTDTAGKAQLAGLDLGLSEDRFLLVDDPAYTELVAAHAVESPAEDPGIDASTLFLLLFTSGTTGTSKAVRCSQGRLARLAYANTAKYGHVREDVDYCCMPLFHGNALMALWAPALANGATVCLPRKFSASGFLPDVRFFGATFFTYVGKALAYLMATPEQPDDRDNTLVRGFGTEASPEDKTEFVRRFGAELYEGYGSSEGAGSVTLDPDAPEGALGRPANENIVIVDPDTRVEKARARLDEHGRVLNPDEAIGEMVDKAGASRFEGYYKNEDAIADRIRHGWYWTGDLGYVDEAGFIYFAGRKGDWIRVDGENTSALMVERILRRHPKVVATGVFAVPDPRSGDQVMAAVEVADPTDFDPAEFAAFLGNQDDLGTKAAPRFVRVSRDLPVTGSNKVLKRTLQEQRWRCDDPVFRWVGRGVPEYHEMTDSEKAVLEQEFHTHGRQRFLHV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000431
EMBL· GenBank· DDBJ
ABG97599.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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