Q0S4D7 · CASI_RHOJR
- ProteinSteroid-22-oyl-CoA synthetase
- GenecasI
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids555 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Involved in cholate catabolism (PubMed:23024343, PubMed:24244004).
Catalyzes the ATP-dependent formation of CoA thioesters of steroids with isopropanoyl side chains, likely occurring as degradation intermediates (PubMed:23024343, PubMed:24244004).
Can use 4-BNC, HSBNC and HIDP as substrate (PubMed:23024343, PubMed:24244004).
Catalyzes the ATP-dependent formation of CoA thioesters of steroids with isopropanoyl side chains, likely occurring as degradation intermediates (PubMed:23024343, PubMed:24244004).
Can use 4-BNC, HSBNC and HIDP as substrate (PubMed:23024343, PubMed:24244004).
Catalytic activity
- 3-oxochol-4-en-22-oate + ATP + CoA = 3-oxochol-4-en-22-oyl-CoA + AMP + diphosphateThis reaction proceeds in the forward direction.
- 3-hydroxy-9-oxo-9,10-seco-chola-1,3,5-trien-22-oate + ATP + CoA = 3-hydroxy-9-oxo-9,10-seco-chola-1,3,5-trien-22-oyl-CoA + AMP + diphosphateThis reaction proceeds in the forward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
75 μM | 4-BNC | |||||
500 μM | HSBNC | |||||
190 μM | HIDP |
kcat is 18 sec-1 with 4-BNC as substrate. kcat is 1.4 sec-1 with HSBNC as substrate. kcat is 60 sec-1 with HIDP as substrate.
Pathway
Steroid metabolism.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | long-chain fatty acid transmembrane transporter activity | |
Molecular Function | long-chain fatty acid-CoA ligase activity | |
Biological Process | steroid metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameSteroid-22-oyl-CoA synthetase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Nocardiaceae > Rhodococcus
Accessions
- Primary accessionQ0S4D7
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000452102 | 1-555 | Steroid-22-oyl-CoA synthetase | ||
Expression
Induction
Up-regulated on cholate.
Structure
Sequence
- Sequence statusComplete
- Length555
- Mass (Da)61,164
- Last updated2006-09-05 v1
- MD5 Checksum3C95E4E2257932F2706B73DB22D47256
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000431 EMBL· GenBank· DDBJ | ABG97599.1 EMBL· GenBank· DDBJ | Genomic DNA |