Q0RM11 · TSAD_FRAAA
- ProteintRNA N6-adenosine threonylcarbamoyltransferase
- GenetsaD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids356 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.
Catalytic activity
- L-threonylcarbamoyladenylate + adenosine37 in tRNA = N6-L-threonylcarbamoyladenosine37 in tRNA + AMP + H+
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 116 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 120 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 139-143 | substrate | ||||
Sequence: IVSGG | ||||||
Binding site | 174 | substrate | ||||
Sequence: D | ||||||
Binding site | 187 | substrate | ||||
Sequence: G | ||||||
Binding site | 191 | substrate | ||||
Sequence: D | ||||||
Binding site | 281 | substrate | ||||
Sequence: N | ||||||
Binding site | 309 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | iron ion binding | |
Molecular Function | N(6)-L-threonylcarbamoyladenine synthase activity | |
Biological Process | tRNA threonylcarbamoyladenosine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA N6-adenosine threonylcarbamoyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Frankiales > Frankiaceae > Frankia
Accessions
- Primary accessionQ0RM11
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000303369 | 1-356 | tRNA N6-adenosine threonylcarbamoyltransferase | |||
Sequence: MPVQPSPVVMGIETSCDETGVALVRDGVLLGDALSTSMDQHARYGGVVPEIAARAHVQALVPCVRAALASAGLTAADIGAVAVTAGPGLATALHVGVSAAKAYATALDVPFYGVHHLAGHLAADLVDGEPLPDPLIALIVSGGHTSLLRVGDLARDPIVHLGDTLDDAAGECFDKVARVLGLPYPGGPAVDREAVGNDPAALAFPRPLTGRTDSPYTFSFSGLKTAVARWVEAHPDSTVPAGDVIASFQEAVVDVLTAKAIRACRDHEIGDLLIVGGVAANSRLRALAAQRCEAAGLRLRIPARKRCTDNGVMIAALGDLLVRSGAAPSRPDLAAMPGAFLDQAQLGVVQPTQRAA |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length356
- Mass (Da)36,377
- Last updated2006-09-05 v1
- Checksum5A66C83B72AFFFA9
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CT573213 EMBL· GenBank· DDBJ | CAJ61441.1 EMBL· GenBank· DDBJ | Genomic DNA |