Q0QFQ2 · GDO2_PSEAC

  • Protein
    Gentisate 1,2 dioxygenase 2
  • Gene
    hbzE
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Involved in the degradation of gentisate. Catalyzes the conversion of gentisate (2,5-dihydroxybenzoate) to maleylpyruvate. Can also use other substrates such as 3-bromogentisate, 3-fluorogentisate, 3-isopropylgentisate, 3-methylgentisate and 3-ethylgentisate, but is unable to utilize gentisate that is substituted at the carbon-4 position.

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Activity regulation

Partially inhibited by Na+, Ca2+ or Mn2+ at concentrations of 5 mM. Presence of 5 mM of Zn2+, Hg2+ or EDTA results in more than 95% inhibition.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
113.38 μMgentisate
29.27 μM3-bromogentisate
50.51 μM3-fluorogentisate
31.28 μM3-isopropylgentisate
54.8 μM3-methylgentisate
11.55 μM3-ethylgentisate
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
17.35 μmol/min/mgwith gentisate as substrate
8.41 μmol/min/mgwith 3-bromogentisate as substrate
20.9 μmol/min/mgwith 3-fluorogentisate as substrate
9.9 μmol/min/mgwith 3-isopropylgentisate as substrate
1.76 μmol/min/mgwith 3-methylgentisate as substrate
6.88 μmol/min/mgwith 3-ethylgentisate as substrate

pH Dependence

Optimum pH is 7.0-8.0.

Temperature Dependence

Optimum temperature is 25 degrees Celsius.

GO annotations

AspectTerm
Molecular Functiongentisate 1,2-dioxygenase activity
Biological Processcatabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Gentisate 1,2 dioxygenase 2
  • EC number
  • Short names
    GDOII

Gene names

    • Name
      hbzE

Organism names

  • Taxonomic identifier
  • Strain
    • NCIMB 9867 / P25X
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    Q0QFQ2

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00004522812-348Gentisate 1,2 dioxygenase 2

Expression

Induction

Strictly inducible. Induced by 3-hydroxybenzoate and gentisate.

Interaction

Subunit

Homotetramer.

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain97-165Cupin type-2

Sequence similarities

Belongs to the gentisate 1,2-dioxygenase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    348
  • Mass (Da)
    38,162
  • Last updated
    2006-09-05 v1
  • Checksum
    0171C2442AFBAC95
MSELNLGTVEDLPKDYYEQLVANNTLPLWPSLRSVLPHGKPARRTRPVIWRYADVRPDLLRAGDLTPIEKAEGRVLVLCNPGLGLENMQVTGSIYIGLQLIQPGETAPNHKHSPSAVRFVIEGKGGYTLVNGEKLPMEKGDLILTPPGMWHQHGHEGDGPVVWLDALDLPLIYGIDASYHIDGEEQTLDKAAGTCTSRYRQSGLLPYSALDRKTSFPLLRFPWQEVRESLKQFAAVTPAGELVQLAYVNPETGAECLPTLGFSAIMLRPGETIRLQRRSASGVLHVVEGAGSVVVDDTRHAFTEADTLAIPTHAAVTLSNASSTEPTYLFMVDDAPLHRKLGIYEVFS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DQ394580
EMBL· GenBank· DDBJ
ABD64513.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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