Q0PCS3 · CHR25_ARATH

Function

function

Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments. Addition of RAD54 overcomes inhibition of DNA strand exchange by RAD51 bound to substrate dsDNA. Species preference in the RAD51 dissociation and DNA strand exchange assays underlines the importance of specific RAD54-RAD51 interactions. RAD51 is unable to release dsDNA upon ATP hydrolysis, leaving it stuck on the heteroduplex DNA product after DNA strand exchange (By similarity).
Involved in DNA repair and mitotic recombination (PubMed:16547115, PubMed:17227544, PubMed:18430956).
Functions in the homologous recombinational DNA repair (RAD52) pathway (PubMed:16547115, PubMed:17227544, PubMed:18430956).
Required for synthesis-dependent strand annealing (SDSA) during double-strand break repair (PubMed:22860689).
Facilitates geminiviral replication (e.g. geminivirus mungbean yellow mosaic virus (MYMV) and tomato leaf curl virus (ToLCV)).

Features

Showing features for binding site.

1910100200300400500600700800900
TypeIDPosition(s)Description
Binding site211-218ATP (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular FunctionATP-dependent chromatin remodeler activity
Molecular FunctionDNA binding
Molecular Functionhelicase activity
Molecular Functionhydrolase activity
Biological Processbiological process involved in interaction with host
Biological Processcell division
Biological Processcellular response to gamma radiation
Biological Processcellular response to metal ion
Biological Processdouble-strand break repair via homologous recombination
Biological Processdouble-strand break repair via synthesis-dependent strand annealing
Biological Processresponse to gamma radiation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein CHROMATIN REMODELING 25
  • EC number
  • Short names
    AtCHR25
  • Alternative names
    • DNA repair and recombination protein RAD54
      (AtRAD54
      )

Gene names

    • Name
      CHR25
    • Synonyms
      RAD54
    • ORF names
      MVI11.13
    • Ordered locus names
      At3g19210

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q0PCS3
  • Secondary accessions
    • F4JB43
    • Q9LJK7

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Increased sensitivity to gamma-irradiation and to the cross-linking reagent cisplatin (PubMed:17227544).
Reduced efficiency of somatic homologous recombination (HR) (PubMed:16547115, PubMed:17227544).
Reduced synthesis-dependent strand annealing (SDSA) frequency (PubMed:22860689).
Impaired geminiviral replication (e.g. tomato leaf curl virus (ToLCV)) (PubMed:22171001).

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 53 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004308591-910Protein CHROMATIN REMODELING 25

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed ubiquitously, with the highest levels of expression in flower buds. Present in flower buds (at protein level).

Induction

Induced by gamma-irradiation and by heavy ion irradiation (PubMed:17227544, PubMed:22683605).
Induced by the genotoxic formaldehyde (FA) (PubMed:20399886).
Accumulates in aerial part of low-energy-ion irradiated dormant plant seeds, thus revealing an abscopal mutagenic effect (PubMed:21557702).

Gene expression databases

Interaction

Subunit

Interacts with RAD51 (PubMed:17227544, PubMed:18430956).
Binds to the geminivirus mungbean yellow mosaic virus (MYMV) and to the tomato leaf curl virus (ToLCV) replication-associated proteins (PubMed:22171001).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q0PCS3RAD51 P941023EBI-1768899, EBI-307687

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain, motif, coiled coil.

TypeIDPosition(s)Description
Region1-39Disordered
Domain198-371Helicase ATP-binding
Motif322-325DEAH box
Coiled coil396-417
Domain538-696Helicase C-terminal
Region828-861Disordered

Sequence similarities

Belongs to the SNF2/RAD54 helicase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q0PCS3-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    910
  • Mass (Da)
    101,858
  • Last updated
    2006-09-19 v1
  • Checksum
    3423B9EC60C0D8AE
MEEEDEEILSSSDCDDSSDSYKDDSQDSEGENDNPECEDLAVVSLSSDADRKSKNVKDLLRGNLVVQRQPLLPRVLSVSDGAAVCRKPFKPPCSHGYDSTGQLSRRLSARKRFVPWGSSTPVVVALPTKLEASTNIERDEEEEVVCLPPDIEPLVLWQSEEDGMSNVTTIMVHSVLVKFLRPHQREGVQFMFDCVSGLHGSANINGCILADDMGLGKTLQSITLLYTLLCQGFDGTPMVKKAIIVTPTSLVSNWEAEIKKWVGDRIQLIALCESTRDDVLSGIDSFTRPRSALQVLIISYETFRMHSSKFCQSESCDLLICDEAHRLKNDQTLTNRALASLTCKRRVLLSGTPMQNDLEEFFAMVNFTNPGSLGDAAHFRHYYEAPIICGREPTATEEEKNLAADRSAELSSKVNQFILRRTNALLSNHLPPKIIEVVCCKMTTLQSTLYNHFISSKNLKRALADNAKQTKVLAYITALKKLCNHPKLIYDTIKSGNPGTVGFENCLEFFPAEMFSGRSGAWTGGDGAWVELSGKMHVLSRLLANLRRKTDDRIVLVSNYTQTLDLFAQLCRERRYPFLRLDGSTTISKRQKLVNRLNDPTKDEFAFLLSSKAGGCGLNLIGANRLVLFDPDWNPANDKQAAARVWRDGQKKRVYVYRFLSTGTIEEKVYQRQMSKEGLQKVIQHEQTDNSTRQGNLLSTEDLRDLFSFHGDVRSEIHEKMSCSRCQNDASGTENIEEGNENNVDDNACQIDQEDIGGFAKDAGCFNLLKNSERQVGTPLEEDLGSWGHHFTSKSVPDAILQASAGDEVTFVFTNQVDGKLVPIESNVSPKTVESEEHNRNQPVNKRAFNKPQQRPREPLQPLSLNETTKRVKLSTYKRLHGNSNIDDAQIKMSLQRPNLVSVNHDDDFV

Q0PCS3-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1I9LM75A0A1I9LM75_ARATHRAD54782

Sequence caution

The sequence BAB02963.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_057104447-458in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB250666
EMBL· GenBank· DDBJ
BAF03042.1
EMBL· GenBank· DDBJ
mRNA
DQ912973
EMBL· GenBank· DDBJ
ABJ99465.1
EMBL· GenBank· DDBJ
mRNA
AP000419
EMBL· GenBank· DDBJ
BAB02963.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002686
EMBL· GenBank· DDBJ
AEE76208.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002686
EMBL· GenBank· DDBJ
AEE76209.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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