Q0PCS3 · CHR25_ARATH
- ProteinProtein CHROMATIN REMODELING 25
- GeneCHR25
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids910 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments. Addition of RAD54 overcomes inhibition of DNA strand exchange by RAD51 bound to substrate dsDNA. Species preference in the RAD51 dissociation and DNA strand exchange assays underlines the importance of specific RAD54-RAD51 interactions. RAD51 is unable to release dsDNA upon ATP hydrolysis, leaving it stuck on the heteroduplex DNA product after DNA strand exchange (By similarity).
Involved in DNA repair and mitotic recombination (PubMed:16547115, PubMed:17227544, PubMed:18430956).
Functions in the homologous recombinational DNA repair (RAD52) pathway (PubMed:16547115, PubMed:17227544, PubMed:18430956).
Required for synthesis-dependent strand annealing (SDSA) during double-strand break repair (PubMed:22860689).
Involved in DNA repair and mitotic recombination (PubMed:16547115, PubMed:17227544, PubMed:18430956).
Functions in the homologous recombinational DNA repair (RAD52) pathway (PubMed:16547115, PubMed:17227544, PubMed:18430956).
Required for synthesis-dependent strand annealing (SDSA) during double-strand break repair (PubMed:22860689).
Facilitates geminiviral replication (e.g. geminivirus mungbean yellow mosaic virus (MYMV) and tomato leaf curl virus (ToLCV)).
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATP-dependent chromatin remodeler activity | |
Molecular Function | DNA binding | |
Molecular Function | helicase activity | |
Molecular Function | hydrolase activity | |
Biological Process | biological process involved in interaction with host | |
Biological Process | cell division | |
Biological Process | cellular response to gamma radiation | |
Biological Process | cellular response to metal ion | |
Biological Process | double-strand break repair via homologous recombination | |
Biological Process | double-strand break repair via synthesis-dependent strand annealing | |
Biological Process | response to gamma radiation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein CHROMATIN REMODELING 25
- EC number
- Short namesAtCHR25
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ0PCS3
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Increased sensitivity to gamma-irradiation and to the cross-linking reagent cisplatin (PubMed:17227544).
Reduced efficiency of somatic homologous recombination (HR) (PubMed:16547115, PubMed:17227544).
Reduced synthesis-dependent strand annealing (SDSA) frequency (PubMed:22860689).
Impaired geminiviral replication (e.g. tomato leaf curl virus (ToLCV)) (PubMed:22171001).
Reduced efficiency of somatic homologous recombination (HR) (PubMed:16547115, PubMed:17227544).
Reduced synthesis-dependent strand annealing (SDSA) frequency (PubMed:22860689).
Impaired geminiviral replication (e.g. tomato leaf curl virus (ToLCV)) (PubMed:22171001).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 53 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000430859 | 1-910 | Protein CHROMATIN REMODELING 25 | |||
Sequence: MEEEDEEILSSSDCDDSSDSYKDDSQDSEGENDNPECEDLAVVSLSSDADRKSKNVKDLLRGNLVVQRQPLLPRVLSVSDGAAVCRKPFKPPCSHGYDSTGQLSRRLSARKRFVPWGSSTPVVVALPTKLEASTNIERDEEEEVVCLPPDIEPLVLWQSEEDGMSNVTTIMVHSVLVKFLRPHQREGVQFMFDCVSGLHGSANINGCILADDMGLGKTLQSITLLYTLLCQGFDGTPMVKKAIIVTPTSLVSNWEAEIKKWVGDRIQLIALCESTRDDVLSGIDSFTRPRSALQVLIISYETFRMHSSKFCQSESCDLLICDEAHRLKNDQTLTNRALASLTCKRRVLLSGTPMQNDLEEFFAMVNFTNPGSLGDAAHFRHYYEAPIICGREPTATEEEKNLAADRSAELSSKVNQFILRRTNALLSNHLPPKIIEVVCCKMTTLQSTLYNHFISSKNLKRALADNAKQTKVLAYITALKKLCNHPKLIYDTIKSGNPGTVGFENCLEFFPAEMFSGRSGAWTGGDGAWVELSGKMHVLSRLLANLRRKTDDRIVLVSNYTQTLDLFAQLCRERRYPFLRLDGSTTISKRQKLVNRLNDPTKDEFAFLLSSKAGGCGLNLIGANRLVLFDPDWNPANDKQAAARVWRDGQKKRVYVYRFLSTGTIEEKVYQRQMSKEGLQKVIQHEQTDNSTRQGNLLSTEDLRDLFSFHGDVRSEIHEKMSCSRCQNDASGTENIEEGNENNVDDNACQIDQEDIGGFAKDAGCFNLLKNSERQVGTPLEEDLGSWGHHFTSKSVPDAILQASAGDEVTFVFTNQVDGKLVPIESNVSPKTVESEEHNRNQPVNKRAFNKPQQRPREPLQPLSLNETTKRVKLSTYKRLHGNSNIDDAQIKMSLQRPNLVSVNHDDDFV |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed ubiquitously, with the highest levels of expression in flower buds. Present in flower buds (at protein level).
Induction
Induced by gamma-irradiation and by heavy ion irradiation (PubMed:17227544, PubMed:22683605).
Induced by the genotoxic formaldehyde (FA) (PubMed:20399886).
Accumulates in aerial part of low-energy-ion irradiated dormant plant seeds, thus revealing an abscopal mutagenic effect (PubMed:21557702).
Induced by the genotoxic formaldehyde (FA) (PubMed:20399886).
Accumulates in aerial part of low-energy-ion irradiated dormant plant seeds, thus revealing an abscopal mutagenic effect (PubMed:21557702).
Gene expression databases
Interaction
Subunit
Interacts with RAD51 (PubMed:17227544, PubMed:18430956).
Binds to the geminivirus mungbean yellow mosaic virus (MYMV) and to the tomato leaf curl virus (ToLCV) replication-associated proteins (PubMed:22171001).
Binds to the geminivirus mungbean yellow mosaic virus (MYMV) and to the tomato leaf curl virus (ToLCV) replication-associated proteins (PubMed:22171001).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q0PCS3 | RAD51 P94102 | 3 | EBI-1768899, EBI-307687 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain, motif, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-39 | Disordered | ||||
Sequence: MEEEDEEILSSSDCDDSSDSYKDDSQDSEGENDNPECED | ||||||
Domain | 198-371 | Helicase ATP-binding | ||||
Sequence: LHGSANINGCILADDMGLGKTLQSITLLYTLLCQGFDGTPMVKKAIIVTPTSLVSNWEAEIKKWVGDRIQLIALCESTRDDVLSGIDSFTRPRSALQVLIISYETFRMHSSKFCQSESCDLLICDEAHRLKNDQTLTNRALASLTCKRRVLLSGTPMQNDLEEFFAMVNFTNPG | ||||||
Motif | 322-325 | DEAH box | ||||
Sequence: DEAH | ||||||
Coiled coil | 396-417 | |||||
Sequence: TEEEKNLAADRSAELSSKVNQF | ||||||
Domain | 538-696 | Helicase C-terminal | ||||
Sequence: VLSRLLANLRRKTDDRIVLVSNYTQTLDLFAQLCRERRYPFLRLDGSTTISKRQKLVNRLNDPTKDEFAFLLSSKAGGCGLNLIGANRLVLFDPDWNPANDKQAAARVWRDGQKKRVYVYRFLSTGTIEEKVYQRQMSKEGLQKVIQHEQTDNSTRQGN | ||||||
Region | 828-861 | Disordered | ||||
Sequence: VSPKTVESEEHNRNQPVNKRAFNKPQQRPREPLQ |
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q0PCS3-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length910
- Mass (Da)101,858
- Last updated2006-09-19 v1
- Checksum3423B9EC60C0D8AE
Q0PCS3-2
- Name2
- Differences from canonical
- 447-458: STLYNHFISSKN → TTYNGCLCMQ
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1I9LM75 | A0A1I9LM75_ARATH | RAD54 | 782 |
Sequence caution
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_057104 | 447-458 | in isoform 2 | |||
Sequence: STLYNHFISSKN → TTYNGCLCMQ |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB250666 EMBL· GenBank· DDBJ | BAF03042.1 EMBL· GenBank· DDBJ | mRNA | ||
DQ912973 EMBL· GenBank· DDBJ | ABJ99465.1 EMBL· GenBank· DDBJ | mRNA | ||
AP000419 EMBL· GenBank· DDBJ | BAB02963.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002686 EMBL· GenBank· DDBJ | AEE76208.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE76209.1 EMBL· GenBank· DDBJ | Genomic DNA |