Q0P897 · CAS9_CAMJE
- ProteinCRISPR-associated endonuclease Cas9
- Genecas9
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids984 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity (By similarity).
Cuts target DNA in Cas9:gRNAs mixing experiments with N.meningitidis strain Z2491 and P.multocoda strain Pm70
Cuts target DNA in Cas9:gRNAs mixing experiments with N.meningitidis strain Z2491 and P.multocoda strain Pm70
Cofactor
Biotechnology
The simplicity of the Cas9-gRNAs RNA-directed DNA endonuclease activity may be used to target and modify a DNA sequence of interest.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 8 | For RuvC-like nuclease domain | |||
Binding site | 8 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 8 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 479 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 483 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 483 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Active site | 559 | Proton acceptor for HNH nuclease domain | |||
Binding site | 707 | Mg2+ 2 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA binding | |
Molecular Function | endonuclease activity | |
Molecular Function | metal ion binding | |
Molecular Function | RNA binding | |
Biological Process | defense response to virus | |
Biological Process | maintenance of CRISPR repeat elements |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameCRISPR-associated endonuclease Cas9
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Campylobacterota > Epsilonproteobacteria > Campylobacterales > Campylobacteraceae > Campylobacter
Accessions
- Primary accessionQ0P897
Proteomes
Phenotypes & Variants
Miscellaneous
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000429983 | 1-984 | CRISPR-associated endonuclease Cas9 | ||
Proteomic databases
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 487-637 | HNH Cas9-type | |||
Domain
Has 2 endonuclease domains. The discontinuous RuvC-like domain cleaves the target DNA noncomplementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA.
Sequence similarities
Belongs to the CRISPR-associated protein Cas9 family. Subtype II-C subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length984
- Mass (Da)114,896
- Last updated2006-09-19 v1
- MD5 ChecksumAFC74900D3653EB31787F4B71048E0C5
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL111168 EMBL· GenBank· DDBJ | CAL35627.1 EMBL· GenBank· DDBJ | Genomic DNA |