Q0JKS6 · Q0JKS6_ORYSJ
- ProteinKetol-acid reductoisomerase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids548 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
Catalytic activity
- (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-acetolactate + NADPH + H+
Cofactor
Note: Binds 2 magnesium ions per subunit.
Pathway
Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.
Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 178 | |||||
Sequence: H | ||||||
Binding site | 267 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 267 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 271 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 444 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 448 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 470 | substrate | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | isomerase activity | |
Molecular Function | ketol-acid reductoisomerase activity | |
Molecular Function | metal ion binding | |
Biological Process | isoleucine biosynthetic process | |
Biological Process | valine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameKetol-acid reductoisomerase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ0JKS6
- Secondary accessions
Proteomes
Genome annotation databases
PTM/Processing
Proteomic databases
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 60-258 | KARI N-terminal Rossmann | ||||
Sequence: VRGGRNLFPLLPEAFKGIKQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSKSFEEARAAGFTEESGTLGDIWETISSSDLLLLLISDAAQADNYEEIFSHMKPNSILGLSHGFLLGHLQSVGLDFPKNISVVAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFAT | ||||||
Domain | 259-407 | KARI C-terminal knotted | ||||
Sequence: TLEQEYRSDIFGERGILLGAVHGIVEALFRRYTEHGLDEDSAYKNTVECITGIISKTISKKGMLEVYNSLTEEGKKQFIEAYSAAYYPCMDILYECYEDVSSGSEIRSVVLAGRRFYEKEGLPAFPMGNIDQTRMWKVGERVRSTRPAG | ||||||
Domain | 408-544 | KARI C-terminal knotted | ||||
Sequence: DLGPLHPFTAGVYVALMMAQIEVLRKKGHSYSEIINESLIESVDSLNPFMHARGVAFMVDNCSTTARLGSRKWAPRFDYILTQQAFVTVDKNAPVNQDLISNFFSDPVHSAIEVCAQLRPTVDISVPADADFVRPEL |
Sequence similarities
Belongs to the ketol-acid reductoisomerase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length548
- Mass (Da)59,682
- Last updated2020-02-26 v1
- Checksum0D81A3EDC712F6E8
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP003710 EMBL· GenBank· DDBJ | BAD68706.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014957 EMBL· GenBank· DDBJ | BAS73463.1 EMBL· GenBank· DDBJ | Genomic DNA |