Q0JKD0 · GLT1_ORYSJ

Function

function

Involved in glutamate biosynthesis and plays a major role in the primary ammonium ions assimilation in seedling roots. May be involved in the reutilization of glutamine in developing organs. Plays a role in the development of tillers.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
[3Fe-4S] cluster (UniProtKB | Rhea| CHEBI:21137 )

Note: Binds 1 [3Fe-4S] cluster.
FAD (UniProtKB | Rhea| CHEBI:57692 )

FMN (UniProtKB | Rhea| CHEBI:58210 )

Pathway

Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NAD+ route): step 1/1.
Energy metabolism; nitrogen metabolism.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site100Nucleophile
Binding site1192-1249FMN (UniProtKB | ChEBI)
Binding site1245[3Fe-4S] cluster (UniProtKB | ChEBI)
Binding site1251[3Fe-4S] cluster (UniProtKB | ChEBI)
Binding site1256[3Fe-4S] cluster (UniProtKB | ChEBI)
Binding site1956-1970NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Cellular Componentplastid
Molecular Function3 iron, 4 sulfur cluster binding
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular Functionglutamate synthase (NADH) activity
Molecular Functioniron ion binding
Biological Processammonia assimilation cycle
Biological Processdevelopmental growth
Biological Processglutamate biosynthetic process
Biological ProcessL-glutamate biosynthetic process
Biological Processresponse to ammonium ion

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glutamate synthase 1 [NADH], chloroplastic
  • EC number
  • Alternative names
    • NADH-dependent glutamate synthase 1 (NADH-GOGAT 1)

Gene names

    • Ordered locus names
      Os01g0681900, LOC_Os01g48960

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Sasanishiki
    • cv. Nipponbare
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa

Accessions

  • Primary accession
    Q0JKD0
  • Secondary accessions
    • Q9ZNX7

Proteomes

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Inhibition of the main root elongation when grown in presence of ammonium chloride. Reduced plant height, biomass and panicle number.

PTM/Processing

Features

Showing features for transit peptide, chain, modified residue (large scale data).

TypeIDPosition(s)SourceDescription
Transit peptide1-36UniProtChloroplast
ChainPRO_000039520137-2167UniProtGlutamate synthase 1 [NADH], chloroplastic
Modified residue (large scale data)214PTMeXchangePhosphoserine

Proteomic databases

Expression

Tissue specificity

Highly expressed in roots.

Induction

By ammonium supply in roots.

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-31Disordered
Domain100-504Glutamine amidotransferase type-2
Region1022-1042Disordered

Sequence similarities

Belongs to the glutamate synthase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    2,167
  • Mass (Da)
    236,895
  • Last updated
    2006-10-03 v1
  • Checksum
    1CDED150F52B00EB
MSAAQGMAYKLRTDAAPTGAGRRARRSHSSVAAPYRAARLVQGGVSIEGGLVGGCQLTEERVAARPPRAAARDAEPVRPLSTLPESSIGLYDPSRERDSCGVGFVAELSGDYKRATVNDALEMLERMAHRGACGCEKNTGDGAGILVALPHNFFREVTKDAGFELPQPGEYAVGMVFLPIDEKRRERSKAEFQKVAESLGHVILGWRRVPTDNSDLGESALQTEPVIEQVFLTKSSSSEADFEQQLYILRRLSILSIRAALNLRRGGKRDFYMCSLSSRTIVYKGQLKPCQLKGYYYADLGHENFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLKGNKNWMKAREGLLECEKLGLTKDQFSKILPIVDATSSDSGAFDGVLELLIRGGRSLPEAVMMMIPEAWQNDVNMEPEKKALYEFLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRVVMGSEVGVVDVPSKDVLRKGRLNPGMMLLVDFENHTVVDDEALKAQYSKAHPYGEWLKRQKIYLKDIVESVPETERVAPGISGSLTQKNEKKEHAGVNGIVTPLKAFGYTVEALEMLLLPMAKDGVEALGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLLETTEKQCNRLALEGPLVSIDEMEAIKKMNYRGWRSKVLDITYPKKSGRKGLEETLDRICTEARGAIKKGYTVLVLSDRGFSSDRVAVSSLLAVGAVHQHLVANLERTRVGLLVESAEPREVHHFCTLVGFGADAVCPYLAIEAIWCLQNDGKIPPNGDGKPYSKEELVKKYFYASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIRKCFDGTPSRIEGATFEMLARDALRLHELAFPSRAPPPGSADAKALPNPGDYHWRKNGEVHLNDPLAMAKLQEAARVNSRAAYKEYSRRIQELNKTCNLRGMLKFKDTADMISVDEVEPASEIVKRFVTGAMSYGSISLEAHTALAMAMNKLGGKSNTGEGGEQPSRMEPLANGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAVTRHSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNSNPRARISVKLVSEAGVGVVASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANGLRGRAILQTDGQLKTGKDVAVACLLGAEEFGFSTAPLITLGCIMMRKCHTNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEELREIMSQLGFRTITEMVGRSDMLEVDPEVVKSNEKLENIDLSLILKPAAEIRPGAAQYCVEKQDHGLDMALDNKLIALSKAALEKEVRVFIETPIQNTNRAVGTMLSHEVTKRYHMKGLPAGTIHVKLTGSAGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKIVVYPPRDSTFIPEDNIVIGNVALYGATIGEAYFNGMAAERFCVRNSGAQAVVEGIGDHGCEYMTGGTVVILGKTGRNFAAGMSGGIAYVYDIDGKFSVRCNHELVDLYHVEEEEDITTLKMMIEQHRLNTGSVVARDILSNFDTLLPKFVKVFPRDYKRVLDNMKAEKAAAKLAKEPKISNGVSVTTKKVQPEQSTNRPTRVSNAKKYRGFISYERESISYRDPNERVKDWKEVAIESVPGPLLNTQSARCMDCGTPFCHQESSGAGCPLGNKIPEFNELVHQNRWREALDRLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKGFEEGWMVPRPPLQRTGKKVAIIGSGPAGLAAADQLNKMGHFVTVFERADRIGGLMMYGVPNMKTDKIEIVQRRVNLMAEEGITFVVNANVGSDPLYSIERLRSENDAVILACGATKPRDLGIPGRELSGVHFAMEFLHANTKSLLDSNLEDGRYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTSIVNLELLTKPPSKRAADNPWPQWPRIFRVDYGHQEASSKFGNDPRTYEVLTKRFIGDENGNVKALEVVRVKWEKVDGRFQFKEIEGSNETIEADLVLLAMGFLGPEATIAEKLGLEKDNRSNFKAQFGNFATSVDGIFAAGDCRRGQSLVVWAITEGRQAAAAVDKYLSRNEQDAAEDITPSGAGFVQPVAA

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict769in Ref. 1; BAA35120
Sequence conflict2056in Ref. 1; BAA35120

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB008845
EMBL· GenBank· DDBJ
BAA35120.1
EMBL· GenBank· DDBJ
mRNA
AP008207
EMBL· GenBank· DDBJ
BAF05798.1
EMBL· GenBank· DDBJ
Genomic DNA
AP014957
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

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