Q0JFZ0 · IRO2_ORYSJ

Function

function

Transcription activator that binds to the DNA motif 5'-CACGTGG-3' in the promoter of iron (Fe) deficiency-inducible genes as well as of genes involved in iron homeostasis, thus contributing to basal tolerance to iron deficiency, iron uptake from soil and iron transport, particularly during seed maturation and germination (PubMed:16887895, PubMed:17559517, PubMed:21331630, PubMed:26224556).
Promotes the accumulation of mugineic acid family phytosiderophores (MAs) (PubMed:17559517).
Required for ethylene-mediated signaling during iron deficiency responses (PubMed:21112958).
Improves growth and yield, especially in calcareous soil with low iron availability. Promotes iron concentration in shoots and grain (PubMed:21331630).

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular ComponentRNA polymerase II transcription regulator complex
Molecular FunctionDNA-binding transcription factor activity
Molecular FunctionDNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Functionprotein dimerization activity
Molecular FunctionRNA polymerase II transcription regulatory region sequence-specific DNA binding
Biological Processethylene-activated signaling pathway
Biological Processpositive regulation of DNA-templated transcription
Biological Processpositive regulation of siderophore biosynthetic process
Biological Processregulation of DNA-templated transcription
Biological Processregulation of ethylene-activated signaling pathway
Biological Processregulation of transcription by RNA polymerase II
Biological Processresponse to arsenic-containing substance
Biological Processresponse to cadmium ion
Biological Processresponse to ethylene
Biological Processresponse to iron ion starvation
Biological Processresponse to nitric oxide

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Protein IRON-RELATED TRANSCRIPTION FACTOR 2
  • Short names
    OsIRO2
  • Alternative names
    • Basic helix-loop-helix protein 56
      (OsbHLH056
      )

Gene names

    • Name
      IRO2
    • Synonyms
      BHLH056
    • ORF names
      OsJ_04787
      , P0431G06.13-1
    • Ordered locus names
      LOC_Os01g72370
      , Os01g0952800

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Nipponbare
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa

Accessions

  • Primary accession
    Q0JFZ0
  • Secondary accessions
    • A9JX09
    • Q941Z7

Proteomes

Genome annotation databases

Subcellular Location

Nucleus
Cytoplasm
Note: Localized partially to the nucleus under iron deficiency conditions.

Keywords

Phenotypes & Variants

Disruption phenotype

Reduced growth compared after germination (PubMed:21331630).
Lower mugineic acid family phytosiderophores (MAs) secretion and hypersensitivity to iron (Fe) deficiency (PubMed:17559517).
Reduced induction of genes involved in iron homeostasis upon iron deficiency and ethylene treatment (PubMed:21112958).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004420631-247

Proteomic databases

Expression

Tissue specificity

Expressed constitutively at low levels in the roots (PubMed:16887895, PubMed:21331630).
Also observed in flowers, developing seeds, embryos and vascular bundles (PubMed:21331630).

Induction

Strongly induced in both roots and shoots during iron (Fe) deficiency stress (PubMed:16887895, PubMed:18025467, PubMed:19737364, PubMed:21112958, PubMed:21331630, PubMed:24280375).
Ethylene enhances the iron deficiency-mediated induction (PubMed:21112958).
Induced by arsenate (AsV25 and AsV50); this induction is repressed by nitric oxide (NO) (PubMed:26793232).
Accumulates under cadmium (Cd) stress; this induction is inhibited by the DNA methyltransferase inhibitor 5-aza-2-deoxycytidine (Aza) (PubMed:27412910).

Developmental stage

During seeds germination, detected in embryos. In vegetative tissues under iron-sufficient, restricted almost exclusively to vascular bundles of roots and leaves, and to the root exodermis. In response to iron deficiency, accumulates in all tissues of roots and leaves.

Gene expression databases

Interaction

Subunit

Forms homodimers (PubMed:31574173).
Interacts with BHLH156 in the nucleus (PubMed:31574173).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif, region, domain.

TypeIDPosition(s)Description
Motif68-75Nuclear localization signal
Region68-81Basic motif
Domain68-119bHLH
Region82-119Helix-loop-helix motif

Sequence similarities

Belongs to the bHLH protein family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    247
  • Mass (Da)
    26,962
  • Last updated
    2006-10-03 v1
  • Checksum
    1BDDDBADCC236C2B
MEQLFVDDPAFASSMSSLEADIFSGAGQLPSSPWLDLDLDDDVQDLSMAPTTANAVSSGYGSGGSGSHRKLSHNAYERDRRKQLNELYSSLRALLPDADHTKLSIPTTVSRVLKYIPELQKQVENLERKKKELTTTSTTNCKPGVLGSQLMSEGMAPIVSATCINDMEIMVQVSLLSNVAGSVLPLSKCIKVLENEGLHFISSSTSSGFGNRTFYSIHLQRSEGTINEECPAFCERLEKVVRNKAKL

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0P0VCZ9A0A0P0VCZ9_ORYSJOs01g0952800180

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict86in Ref. 1; FAA00382
Sequence conflict95in Ref. 1; FAA00382
Sequence conflict101in Ref. 2; BAB64701 and 6; EAZ14859

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BR000688
EMBL· GenBank· DDBJ
FAA00382.1
EMBL· GenBank· DDBJ
mRNA
AP003683
EMBL· GenBank· DDBJ
BAB64701.1
EMBL· GenBank· DDBJ
Genomic DNA
AP008207
EMBL· GenBank· DDBJ
BAF07338.1
EMBL· GenBank· DDBJ
Genomic DNA
AP014957
EMBL· GenBank· DDBJ
BAS76260.1
EMBL· GenBank· DDBJ
Genomic DNA
CM000138
EMBL· GenBank· DDBJ
EAZ14859.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp