Q0JFZ0 · IRO2_ORYSJ
- ProteinProtein IRON-RELATED TRANSCRIPTION FACTOR 2
- GeneIRO2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids247 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcription activator that binds to the DNA motif 5'-CACGTGG-3' in the promoter of iron (Fe) deficiency-inducible genes as well as of genes involved in iron homeostasis, thus contributing to basal tolerance to iron deficiency, iron uptake from soil and iron transport, particularly during seed maturation and germination (PubMed:16887895, PubMed:17559517, PubMed:21331630, PubMed:26224556).
Promotes the accumulation of mugineic acid family phytosiderophores (MAs) (PubMed:17559517).
Required for ethylene-mediated signaling during iron deficiency responses (PubMed:21112958).
Improves growth and yield, especially in calcareous soil with low iron availability. Promotes iron concentration in shoots and grain (PubMed:21331630).
Promotes the accumulation of mugineic acid family phytosiderophores (MAs) (PubMed:17559517).
Required for ethylene-mediated signaling during iron deficiency responses (PubMed:21112958).
Improves growth and yield, especially in calcareous soil with low iron availability. Promotes iron concentration in shoots and grain (PubMed:21331630).
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameProtein IRON-RELATED TRANSCRIPTION FACTOR 2
- Short namesOsIRO2
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ0JFZ0
- Secondary accessions
Proteomes
Genome annotation databases
Phenotypes & Variants
Disruption phenotype
Reduced growth compared after germination (PubMed:21331630).
Lower mugineic acid family phytosiderophores (MAs) secretion and hypersensitivity to iron (Fe) deficiency (PubMed:17559517).
Reduced induction of genes involved in iron homeostasis upon iron deficiency and ethylene treatment (PubMed:21112958).
Lower mugineic acid family phytosiderophores (MAs) secretion and hypersensitivity to iron (Fe) deficiency (PubMed:17559517).
Reduced induction of genes involved in iron homeostasis upon iron deficiency and ethylene treatment (PubMed:21112958).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000442063 | 1-247 | Protein IRON-RELATED TRANSCRIPTION FACTOR 2 | |||
Sequence: MEQLFVDDPAFASSMSSLEADIFSGAGQLPSSPWLDLDLDDDVQDLSMAPTTANAVSSGYGSGGSGSHRKLSHNAYERDRRKQLNELYSSLRALLPDADHTKLSIPTTVSRVLKYIPELQKQVENLERKKKELTTTSTTNCKPGVLGSQLMSEGMAPIVSATCINDMEIMVQVSLLSNVAGSVLPLSKCIKVLENEGLHFISSSTSSGFGNRTFYSIHLQRSEGTINEECPAFCERLEKVVRNKAKL |
Proteomic databases
Expression
Tissue specificity
Expressed constitutively at low levels in the roots (PubMed:16887895, PubMed:21331630).
Also observed in flowers, developing seeds, embryos and vascular bundles (PubMed:21331630).
Also observed in flowers, developing seeds, embryos and vascular bundles (PubMed:21331630).
Induction
Strongly induced in both roots and shoots during iron (Fe) deficiency stress (PubMed:16887895, PubMed:18025467, PubMed:19737364, PubMed:21112958, PubMed:21331630, PubMed:24280375).
Ethylene enhances the iron deficiency-mediated induction (PubMed:21112958).
Induced by arsenate (AsV25 and AsV50); this induction is repressed by nitric oxide (NO) (PubMed:26793232).
Accumulates under cadmium (Cd) stress; this induction is inhibited by the DNA methyltransferase inhibitor 5-aza-2-deoxycytidine (Aza) (PubMed:27412910).
Ethylene enhances the iron deficiency-mediated induction (PubMed:21112958).
Induced by arsenate (AsV25 and AsV50); this induction is repressed by nitric oxide (NO) (PubMed:26793232).
Accumulates under cadmium (Cd) stress; this induction is inhibited by the DNA methyltransferase inhibitor 5-aza-2-deoxycytidine (Aza) (PubMed:27412910).
Developmental stage
During seeds germination, detected in embryos. In vegetative tissues under iron-sufficient, restricted almost exclusively to vascular bundles of roots and leaves, and to the root exodermis. In response to iron deficiency, accumulates in all tissues of roots and leaves.
Gene expression databases
Structure
Family & Domains
Features
Showing features for motif, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 68-75 | Nuclear localization signal | ||||
Sequence: HRKLSHNA | ||||||
Region | 68-81 | Basic motif | ||||
Sequence: HRKLSHNAYERDRR | ||||||
Domain | 68-119 | bHLH | ||||
Sequence: HRKLSHNAYERDRRKQLNELYSSLRALLPDADHTKLSIPTTVSRVLKYIPEL | ||||||
Region | 82-119 | Helix-loop-helix motif | ||||
Sequence: KQLNELYSSLRALLPDADHTKLSIPTTVSRVLKYIPEL |
Sequence similarities
Belongs to the bHLH protein family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length247
- Mass (Da)26,962
- Last updated2006-10-03 v1
- Checksum1BDDDBADCC236C2B
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0P0VCZ9 | A0A0P0VCZ9_ORYSJ | Os01g0952800 | 180 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 86 | in Ref. 1; FAA00382 | ||||
Sequence: E → K | ||||||
Sequence conflict | 95 | in Ref. 1; FAA00382 | ||||
Sequence: L → I | ||||||
Sequence conflict | 101 | in Ref. 2; BAB64701 and 6; EAZ14859 | ||||
Sequence: T → TK |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BR000688 EMBL· GenBank· DDBJ | FAA00382.1 EMBL· GenBank· DDBJ | mRNA | ||
AP003683 EMBL· GenBank· DDBJ | BAB64701.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP008207 EMBL· GenBank· DDBJ | BAF07338.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014957 EMBL· GenBank· DDBJ | BAS76260.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CM000138 EMBL· GenBank· DDBJ | EAZ14859.1 EMBL· GenBank· DDBJ | Genomic DNA |