Q0JF01 · C99A3_ORYSJ
- Protein9-beta-pimara-7,15-diene oxidase
- GeneCYP99A3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids502 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in momilactone phytoalexins biosynthesis; acts as a multifunctional diterpene oxidase. Participates in the biosynthetic steps between 9-beta-pimara-7,15-diene and 3-beta-hydroxy-9-beta-pimara-7,15-dien-19,6-beta-olide. Catalyzes also consecutive oxidations at C19 of syn-stemod-1317-ene.
Miscellaneous
3-beta-hydroxy-9-beta-pimara-7,15-dien-19,6-beta-olide is a precursor of the phytoalexins momilactones A and B. Phytoalexins are diterpenoid secondary metabolites involved in the defense mechanism of the plant and produced in response to attack (by a pathogen, elicitor or UV irradiation). Momilactone B can also act as an allochemical (an antimicrobial and allelopathic agent), being constitutively produced in the root of the plant and secreted to the rhizosphere where it suppresses the growth of neighboring plants and soil microorganisms.
Catalytic activity
- 9beta-pimara-7,15-diene + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = 9beta-pimara-7,15-dien-19-oate + 4 H+ + 4 H2O + 3 oxidized [NADPH--hemoprotein reductase]
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
2 μM | syn-pimaradiene | |||||
9 μM | syn-stemodene |
kcat is 46 sec-1 with syn-pimaradiene as substrate and 49 sec-1 with syn-stemodene as substrate.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | 9beta-pimara-7,15-diene oxidase activity | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | oxidoreductase activity | |
Biological Process | cellular response to jasmonic acid stimulus | |
Biological Process | defense response | |
Biological Process | diterpenoid metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name9-beta-pimara-7,15-diene oxidase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ0JF01
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 4-26 | Helical | ||||
Sequence: INSEATVTLVSVVTLPILLALLT | ||||||
Transmembrane | 106-128 | Helical | ||||
Sequence: LLVSEIFCYGNLDIGFAPYGAYW |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000349107 | 1-502 | 9-beta-pimara-7,15-diene oxidase | |||
Sequence: MMEINSEATVTLVSVVTLPILLALLTRKSSSKKRRPPGPWNLPLVGGLLHLLRSQPQVALRDLAGKYGPVMFLRTGQVDTVVISSPAAAQEVLRDKDVTFASRPSLLVSEIFCYGNLDIGFAPYGAYWRMLRKLCTVELLSTKMVRQLAPIRDGETLALVRNIEAAAGGKKPFTLATLLISCTNTFTAKAAFGQACGGELQEQFLTALDEALKFSNGFCFGDLFPSLRFIDAMTGLRSRLERLRLQLDTVFDKIVAQCESNPGDSLVNVLLRIKDQGELDFPFSSTHVKAIILDMFTGGTETTSSTTEWLMSELMRNPEVMAKVQAEVRGVFDNKSPQDHEGLLENLSYMKLVIKETLRLNPVLPLLLPHLCRETCEIGGYEIVEGTRVLINSWAMARSPEYWDDAEKFIPERFEDGTADFKGSRFEYLPFGTGRRRCPGDIFAMATLELIVARLLYYFDWSLPDGMQPGDIDMELVVGATARRKNHLQLVASPYKPISMQS |
Proteomic databases
Expression
Induction
By chitin oligosaccharide elicitor and jasmonic acid (MeJA).
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length502
- Mass (Da)55,892
- Last updated2006-10-03 v1
- ChecksumFB572E00D9997A01
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL662933 EMBL· GenBank· DDBJ | CAE04106.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
AP008210 EMBL· GenBank· DDBJ | BAF14086.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014960 EMBL· GenBank· DDBJ | BAS87950.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CM000141 EMBL· GenBank· DDBJ | EAZ29932.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
AK071864 EMBL· GenBank· DDBJ | BAG92737.1 EMBL· GenBank· DDBJ | mRNA |