Q0JF01 · C99A3_ORYSJ

Function

function

Involved in momilactone phytoalexins biosynthesis; acts as a multifunctional diterpene oxidase. Participates in the biosynthetic steps between 9-beta-pimara-7,15-diene and 3-beta-hydroxy-9-beta-pimara-7,15-dien-19,6-beta-olide. Catalyzes also consecutive oxidations at C19 of syn-stemod-1317-ene.

Miscellaneous

3-beta-hydroxy-9-beta-pimara-7,15-dien-19,6-beta-olide is a precursor of the phytoalexins momilactones A and B. Phytoalexins are diterpenoid secondary metabolites involved in the defense mechanism of the plant and produced in response to attack (by a pathogen, elicitor or UV irradiation). Momilactone B can also act as an allochemical (an antimicrobial and allelopathic agent), being constitutively produced in the root of the plant and secreted to the rhizosphere where it suppresses the growth of neighboring plants and soil microorganisms.

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
2 μMsyn-pimaradiene
9 μMsyn-stemodene
kcat is 46 sec-1 with syn-pimaradiene as substrate and 49 sec-1 with syn-stemodene as substrate.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site438Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Function9beta-pimara-7,15-diene oxidase activity
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionoxidoreductase activity
Biological Processcellular response to jasmonic acid stimulus
Biological Processdefense response
Biological Processditerpenoid metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    9-beta-pimara-7,15-diene oxidase
  • EC number
  • Alternative names
    • Cytochrome P450 99A3

Gene names

    • Name
      CYP99A3
    • ORF names
      OsJ_013415, OSJNBa0096F01.14
    • Ordered locus names
      Os04g0178400, LOC_Os04g09920

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Nipponbare
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa

Accessions

  • Primary accession
    Q0JF01
  • Secondary accessions
    • A0A0P0W6Z1
    • A3ARJ3
    • B7EK40
    • Q5JQE4

Proteomes

Genome annotation databases

Subcellular Location

Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane4-26Helical
Transmembrane106-128Helical

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003491071-5029-beta-pimara-7,15-diene oxidase

Proteomic databases

Expression

Induction

By chitin oligosaccharide elicitor and jasmonic acid (MeJA).

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    502
  • Mass (Da)
    55,892
  • Last updated
    2006-10-03 v1
  • Checksum
    FB572E00D9997A01
MMEINSEATVTLVSVVTLPILLALLTRKSSSKKRRPPGPWNLPLVGGLLHLLRSQPQVALRDLAGKYGPVMFLRTGQVDTVVISSPAAAQEVLRDKDVTFASRPSLLVSEIFCYGNLDIGFAPYGAYWRMLRKLCTVELLSTKMVRQLAPIRDGETLALVRNIEAAAGGKKPFTLATLLISCTNTFTAKAAFGQACGGELQEQFLTALDEALKFSNGFCFGDLFPSLRFIDAMTGLRSRLERLRLQLDTVFDKIVAQCESNPGDSLVNVLLRIKDQGELDFPFSSTHVKAIILDMFTGGTETTSSTTEWLMSELMRNPEVMAKVQAEVRGVFDNKSPQDHEGLLENLSYMKLVIKETLRLNPVLPLLLPHLCRETCEIGGYEIVEGTRVLINSWAMARSPEYWDDAEKFIPERFEDGTADFKGSRFEYLPFGTGRRRCPGDIFAMATLELIVARLLYYFDWSLPDGMQPGDIDMELVVGATARRKNHLQLVASPYKPISMQS

Sequence caution

The sequence CAE04106.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.
The sequence EAZ29932.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL662933
EMBL· GenBank· DDBJ
CAE04106.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
AP008210
EMBL· GenBank· DDBJ
BAF14086.1
EMBL· GenBank· DDBJ
Genomic DNA
AP014960
EMBL· GenBank· DDBJ
BAS87950.1
EMBL· GenBank· DDBJ
Genomic DNA
CM000141
EMBL· GenBank· DDBJ
EAZ29932.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
AK071864
EMBL· GenBank· DDBJ
BAG92737.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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