Q0JDC5 · INV2_ORYSJ

Function

function

Cell wall-associated invertase that cleaves sucrose into glucose and fructose and is required for assimilated carbon partitioning during early grain-filling. May be involved in sucrose unloaded in the ovular and stylar vascular tissues for the stimulation of starch synthesis in the developing endosperm during grain-filling (PubMed:18820698).
Sugar homeostasis mediated by CIN2/GIF1 plays an important role in constitutive and induced physical and chemical defense against pathogens (PubMed:24118770).

Catalytic activity

  • Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
    EC:3.2.1.26 (UniProtKB | ENZYME | Rhea)

Biotechnology

CIN2/GIF1 is a potential domestication-selected gene that controls grain filling and yield, and could be used for further crop improvement.

Features

Showing features for active site.

159850100150200250300350400450500550
TypeIDPosition(s)Description
Active site69

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functionbeta-fructofuranosidase activity
Biological Processcarbohydrate metabolic process
Biological Processcarbohydrate storage
Biological Processdefense response to bacterium
Biological Processdefense response to fungus

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Beta-fructofuranosidase, insoluble isoenzyme 2
  • EC number
  • Alternative names
    • Cell wall beta-fructosidase 2
    • Invertase 2
    • OsCIN2
    • Protein GRAIN INCOMPLETE FILLING 1
    • Sucrose hydrolase 2

Gene names

    • Name
      CIN2
    • Synonyms
      GIF1
    • ORF names
      OJ000126_13.8, OsJ_014165
    • Ordered locus names
      Os04g0413500, LOC_Os04g33740

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Nipponbare
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa

Accessions

  • Primary accession
    Q0JDC5
  • Secondary accessions
    • A3ATP3
    • Q56UD4
    • Q6VGJ3
    • Q7XVJ4

Proteomes

Genome annotation databases

Subcellular Location

Note: Associated with the cell wall.

Keywords

Phenotypes & Variants

Disruption phenotype

Slow grain-filling resulting in reduced weight of grains containing loosely packed starch granules and reduced levels of amylose and amylopectin.

PTM/Processing

Features

Showing features for signal, chain, glycosylation.

TypeIDPosition(s)Description
Signal1-25
ChainPRO_000003338026-598Beta-fructofuranosidase, insoluble isoenzyme 2
Glycosylation164N-linked (GlcNAc...) asparagine
Glycosylation189N-linked (GlcNAc...) asparagine
Glycosylation348N-linked (GlcNAc...) asparagine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in leaves and flowers. Weakly expressed in seeds (PubMed:15759120).
Expressed in growing roots, node and the rapidly elongating zone of the internode (PubMed:18820698).

Induction

By sucrose in caryopsis from 1 to 2 and from 9 to 10 days after flowering.

Developmental stage

Expressed throughout flowering, with higher expression from 1 to 6 days after flowering (PubMed:15759120).
During early grain-filling, expressed in the ovular vascular and lateral stylar vascular traces (PubMed:18820698).

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the glycosyl hydrolase 32 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    598
  • Mass (Da)
    66,263
  • Last updated
    2006-10-03 v1
  • Checksum
    DFAE59481FA19A4F
MGVLGSRVAWAWLVQLLLLQQLAGASHVVYDDLELQAAATTADGVPPSIVDSELRTGYHFQPPKNWINDPNAPMYYKGWYHLFYQYNPKGAVWGNIVWAHSVSRDLINWVALKPAIEPSIRADKYGCWSGSATMMADGTPVIMYTGVNRPDVNYQVQNVALPRNGSDPLLREWVKPGHNPVIVPEGGINATQFRDPTTAWRGADGHWRLLVGSLAGQSRGVAYVYRSRDFRRWTRAAQPLHSAPTGMWECPDFYPVTADGRREGVDTSSAVVDAAASARVKYVLKNSLDLRRYDYYTVGTYDRKAERYVPDDPAGDEHHIRYDYGNFYASKTFYDPAKRRRILWGWANESDTAADDVAKGWAGIQAIPRKVWLDPSGKQLLQWPIEEVERLRGKWPVILKDRVVKPGEHVEVTGLQTAQADVEVSFEVGSLEAAERLDPAMAYDAQRLCSARGADARGGVGPFGLWVLASAGLEEKTAVFFRVFRPAARGGGAGKPVVLMCTDPTKSSRNPNMYQPTFAGFVDTDITNGKISLRSLIDRSVVESFGAGGKACILSRVYPSLAIGKNARLYVFNNGKAEIKVSQLTAWEMKKPVMMNGA

Sequence caution

The sequence CAD40589.2 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY578159
EMBL· GenBank· DDBJ
AAT84402.1
EMBL· GenBank· DDBJ
mRNA
AL662945
EMBL· GenBank· DDBJ
CAD40589.2
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AP008210
EMBL· GenBank· DDBJ
BAF14662.1
EMBL· GenBank· DDBJ
Genomic DNA
AP014960
EMBL· GenBank· DDBJ
BAS89136.1
EMBL· GenBank· DDBJ
Genomic DNA
CM000141
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp