Q0J954 · PAO5_ORYSJ
- ProteinPolyamine oxidase 5
- GenePAO5
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids492 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Flavoenzyme involved in polyamine back-conversion (PubMed:21796433).
Catalyzes the oxidation of the secondary amino group of polyamines, such as spermine (PubMed:21796433).
Substrate preference is spermine > thermospermine > norspermine (PubMed:21796433).
No activity detected when putrescine, spermidine or N1-acetylspermidine are used as substrates (PubMed:21796433).
Plays an important role in the regulation of polyamine intracellular concentration (Probable). May play a role in producing hydrogen peroxide during seed germination (Probable)
Catalyzes the oxidation of the secondary amino group of polyamines, such as spermine (PubMed:21796433).
Substrate preference is spermine > thermospermine > norspermine (PubMed:21796433).
No activity detected when putrescine, spermidine or N1-acetylspermidine are used as substrates (PubMed:21796433).
Plays an important role in the regulation of polyamine intracellular concentration (Probable). May play a role in producing hydrogen peroxide during seed germination (Probable)
Catalytic activity
- H2O + O2 + spermine = 3-aminopropanal + H2O2 + spermidine
- H2O + norspermine + O2 = 3-aminopropanal + H2O2 + norspermidine
- H2O + O2 + thermospermine = 3-aminopropanal + H2O2 + spermidine
Cofactor
Note: Binds 1 FAD per subunit.
Pathway
Amine and polyamine degradation; spermine degradation.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | peroxisome | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | norspermine:oxygen oxidoreductase activity | |
Molecular Function | polyamine oxidase activity | |
Molecular Function | spermine:oxygen oxidoreductase (spermidine-forming) activity | |
Biological Process | polyamine catabolic process | |
Biological Process | spermine catabolic process | |
Biological Process | thermospermine catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePolyamine oxidase 5
- EC number
- Short namesOsPAO5
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ0J954
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000445724 | 1-492 | Polyamine oxidase 5 | |||
Sequence: MDQPSNGFAAGGLFLRHIDGQNASPPSVIVIGGGISGIAAARALSNASFKVTLLESRDRLGGRVHTDYSFGCPIDMGASWLHGVCNENSLAPLIRLLGLRLYRTSGDNSVLYDHDLESYALFDKDGRQVPQEIVTKVGETFEKILKETVKVRAEHEDDMPLIQAISIVLDRNPHLKLDGLQYEVLQWCICRLEAWFATDVDNISLKNWDQEHVLTGGHGLMVHGYDPVIKALAQDLDIHLNHRVTKIIQRYNKTIVCVEDGTSFVADAAIITVPLGVLKANIIKFEPELPDWKLSSISDLGIGIENKIALRFNSVFWPNVEVLGRVAPTSNACGYFLNLHKATGHPVLVCMVAGRFAYEFEKLSDEESVNFVMSQLKKMLPGATEPVQYLVSRWGTDPNSLGSYSCDLVGKPADLYERFCAPVGNLFFAGEAACIDHSGSVHGAYSSGIVAAEDCRRHLSTQLGISDLFQVGKIIMREEMTEVMVPFQISRL |
Proteomic databases
Expression
Tissue specificity
Widely expressed.
Developmental stage
During seed germination, expression increases in the embryo from 3 to 48 hours after seed imbibition, with a peak at 48 hours.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 490-492 | Microbody targeting signal | ||||
Sequence: SRL |
Sequence similarities
Belongs to the flavin monoamine oxidase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length492
- Mass (Da)54,186
- Last updated2006-10-03 v1
- ChecksumCD7603D222CDEEFD
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL606652 EMBL· GenBank· DDBJ | CAE03599.2 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AP008210 EMBL· GenBank· DDBJ | BAF16133.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014960 EMBL· GenBank· DDBJ | BAS91567.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CM000141 EMBL· GenBank· DDBJ | EEE61884.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK072414 EMBL· GenBank· DDBJ | BAG92960.1 EMBL· GenBank· DDBJ | mRNA |