Q0DM51 · RH3_ORYSJ
- ProteinDEAD-box ATP-dependent RNA helicase 3, chloroplastic
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids758 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Nuclear genome-encoded factor involved in ribosome biogenesis in chloroplasts. Binds specific group II introns in chloroplasts and facilitates their splicing. Required for normal development of chloroplasts.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA helicase activity | |
Molecular Function | zinc ion binding | |
Biological Process | chloroplast organization | |
Biological Process | Group II intron splicing | |
Biological Process | ribosome biogenesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDEAD-box ATP-dependent RNA helicase 3, chloroplastic
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ0DM51
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Transit peptide | 1-62 | UniProt | Chloroplast | ||||
Sequence: MASLLTLPSLSLSNPSASAAAAGAGAAPSLRLRAAFRCWALRRAGGGRWAAAGAIASPNSVL | |||||||
Chain | PRO_0000282473 | 63-758 | UniProt | DEAD-box ATP-dependent RNA helicase 3, chloroplastic | |||
Sequence: SEHAFKRLQLSDEEEEEEEGAYGSDEEGVEAVGGGEGDEDELAIARLGLPEQLVSTLEKRGITHLFPIQRAVLIPALDGRDLIARAKTGTGKTLAFGIPMIKQLMEEDDGRSVRRGRIPRVLVLAPTRELAKQVEKEIKESAPKLSTVCVYGGVSYNVQQNALSRGVDVVVGTPGRIIDLINGGSLQLGEVKYLVLDEADQMLAVGFEEDVETILQQLPAERQSMLFSATMPGWVKKLSRRYLNNPLTIDLVGDQDEKLAEGIKLYAIPLTSTSKRTVLSDLITVYAKGGKTIVFTKTKRDADEVSLALTNSIASEALHGDISQHQRERTLNGFRQGKFTVLVATDVAARGLDIPNVDLIIHYELPNDPETFVHRSGRTGRAGKAGTAILMFTNSQRRTVRSLERDVGCRFDFISPPAIEDVLESSAEHVIATLRGVHTESIQYFIPAAERLQEELGPNALASALAHLSGFSQPPSSRSLISHEQGWVTLQLTRDPGYGRGFFSPRSVTGFLSDVSSAAADEVGKIFLTADEKVQGAVFDLPEEIARDLLSMELPPGNTITKVTKLPALQDDGPATDSYGRFSNSDRGFRNRRSRGGGSRGGRGGWDSDGEDRFRRGGRSFRSDNDSWSDDDFGGGRRSNRSSSFGGRGSSYGSRSSSSFGGRSSSFGSRDSSRSFSGACFNCGESGHRASDCPNK | |||||||
Modified residue (large scale data) | 691 | PTMeXchange | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 737 | PTMeXchange | Phosphoserine | ||||
Sequence: S |
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, motif, domain, zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 71-97 | Disordered | ||||
Sequence: QLSDEEEEEEEGAYGSDEEGVEAVGGG | ||||||
Compositional bias | 74-97 | Acidic residues | ||||
Sequence: DEEEEEEEGAYGSDEEGVEAVGGG | ||||||
Motif | 104-132 | Q motif | ||||
Sequence: LAIARLGLPEQLVSTLEKRGITHLFPIQR | ||||||
Domain | 135-311 | Helicase ATP-binding | ||||
Sequence: LIPALDGRDLIARAKTGTGKTLAFGIPMIKQLMEEDDGRSVRRGRIPRVLVLAPTRELAKQVEKEIKESAPKLSTVCVYGGVSYNVQQNALSRGVDVVVGTPGRIIDLINGGSLQLGEVKYLVLDEADQMLAVGFEEDVETILQQLPAERQSMLFSATMPGWVKKLSRRYLNNPLTI | ||||||
Motif | 259-262 | DEAD box | ||||
Sequence: DEAD | ||||||
Domain | 340-485 | Helicase C-terminal | ||||
Sequence: VLSDLITVYAKGGKTIVFTKTKRDADEVSLALTNSIASEALHGDISQHQRERTLNGFRQGKFTVLVATDVAARGLDIPNVDLIIHYELPNDPETFVHRSGRTGRAGKAGTAILMFTNSQRRTVRSLERDVGCRFDFISPPAIEDVL | ||||||
Region | 625-737 | Disordered | ||||
Sequence: VTKLPALQDDGPATDSYGRFSNSDRGFRNRRSRGGGSRGGRGGWDSDGEDRFRRGGRSFRSDNDSWSDDDFGGGRRSNRSSSFGGRGSSYGSRSSSSFGGRSSSFGSRDSSRS | ||||||
Compositional bias | 667-699 | Basic and acidic residues | ||||
Sequence: GWDSDGEDRFRRGGRSFRSDNDSWSDDDFGGGR | ||||||
Compositional bias | 700-737 | Polar residues | ||||
Sequence: RSNRSSSFGGRGSSYGSRSSSSFGGRSSSFGSRDSSRS | ||||||
Zinc finger | 740-757 | CCHC-type | ||||
Sequence: GACFNCGESGHRASDCPN |
Domain
The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Sequence similarities
Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 3 isoforms produced by Alternative splicing.
Q0DM51-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length758
- Mass (Da)81,615
- Last updated2007-04-03 v2
- ChecksumE2E6486829E73467
Q0DM51-2
- Name2
- Differences from canonical
- 1-161: Missing
Q0DM51-3
- Name3
- NoteMay be due to a competing acceptor splice site.
- Differences from canonical
- 735-735: Missing
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0P0W5N0 | A0A0P0W5N0_ORYSJ | Os03g0827700 | 433 |
Sequence caution
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_024162 | 1-161 | in isoform 2 | |||
Sequence: Missing | ||||||
Compositional bias | 74-97 | Acidic residues | ||||
Sequence: DEEEEEEEGAYGSDEEGVEAVGGG | ||||||
Compositional bias | 667-699 | Basic and acidic residues | ||||
Sequence: GWDSDGEDRFRRGGRSFRSDNDSWSDDDFGGGR | ||||||
Compositional bias | 700-737 | Polar residues | ||||
Sequence: RSNRSSSFGGRGSSYGSRSSSSFGGRSSSFGSRDSSRS | ||||||
Alternative sequence | VSP_024163 | 735 | in isoform 3 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC096687 EMBL· GenBank· DDBJ | AAL79753.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DP000009 EMBL· GenBank· DDBJ | ABF99667.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DP000009 EMBL· GenBank· DDBJ | ABF99668.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP008209 EMBL· GenBank· DDBJ | BAF13687.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AP014959 EMBL· GenBank· DDBJ | BAS87169.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AK065849 EMBL· GenBank· DDBJ | BAG89705.1 EMBL· GenBank· DDBJ | mRNA |