Q09995 · Q09995_CAEEL
- ProteinPoly(A) polymerase
- Genepap-2
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids554 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Polymerase that creates the 3'-poly(A) tail of mRNA's.
Catalytic activity
- ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide
Cofactor
Protein has several cofactor binding sites:
Note: Binds 2 magnesium ions. Also active with manganese.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 93-95 | ATP (UniProtKB | ChEBI) | ||||
Sequence: FGS | ||||||
Binding site | 106 | Mg2+ 2 (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 106 | Mg2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 106-108 | ATP (UniProtKB | ChEBI) | ||||
Sequence: DID | ||||||
Binding site | 108 | Mg2+ 2 (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 108 | Mg2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 160 | ATP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 160 | Mg2+ 2 (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 221 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 230 | ATP (UniProtKB | ChEBI) | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | poly(A) RNA polymerase activity | |
Molecular Function | RNA binding | |
Biological Process | mRNA processing | |
Biological Process | RNA 3'-end processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePoly(A) polymerase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ09995
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Proteomic databases
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 15-207 | Poly(A) polymerase nucleotidyltransferase | ||||
Sequence: GVSQPISTAFPKANDVALTSSLQSSLEEFKSFESKQETLLRIKVLKNLDGLVKDWVQKTTISRIPKDQMFNAGGKLIPFGSYRLGVHSSGADIDSIVVAPRHITRSDFFNSFKDILAKNPEVTELCAVEKAFVPIMTLKYSGVDIDILFARLALKSVPEDLNILDDSLLKNLDEESVRSLNGCRVAEQLLKLV | ||||||
Domain | 212-361 | Poly(A) polymerase central | ||||
Sequence: NFCITLRAIKLWAKNHGIYSNSMGFFGGITWAILVARICQLYPNAAPSRLIQKVFFIFSTWNWPAPVLLDYINCDRTDLAQLNQLVWDPRRNHADRYHLMPIITPAFPQQNSTHNVSRSSMKVIQDEMKKALIICDKIHEGTLEWRDLLE | ||||||
Domain | 364-425 | Poly(A) polymerase RNA-binding | ||||
Sequence: NFFSKYHHFIALKLKAESVKEELAFGGFFESRIRQLVQILEKNQVIQVAQINPRKFKDVKDP |
Sequence similarities
Belongs to the poly(A) polymerase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length554
- Mass (Da)63,008
- Last updated1996-11-01 v1
- Checksum92573DD4DEF9BE82
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX284602 EMBL· GenBank· DDBJ | CAA87419.1 EMBL· GenBank· DDBJ | Genomic DNA |