Q09388 · ACM2_CAEEL
- ProteinMuscarinic acetylcholine receptor gar-2
- Genegar-2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids627 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins (PubMed:11032868, PubMed:11700045).
Primary transducing effect is Pi turnover (By similarity).
Regulates the activity of ventral cord motor neurons (PubMed:18614679).
Couples to the G(o)-alpha G-protein subunit goa-1 to negatively regulate cholinergic receptor activity in the presence of high levels of the neurotransmitter acetylcholine in ventral cord motor neurons (PubMed:18614679).
As acetylcholine depolarizes body wall muscles, modulation of acetylcholine levels most likely results in the control locomotory behavior and egg-laying (PubMed:12954868, PubMed:18614679).
Primary transducing effect is Pi turnover (By similarity).
Regulates the activity of ventral cord motor neurons (PubMed:18614679).
Couples to the G(o)-alpha G-protein subunit goa-1 to negatively regulate cholinergic receptor activity in the presence of high levels of the neurotransmitter acetylcholine in ventral cord motor neurons (PubMed:18614679).
As acetylcholine depolarizes body wall muscles, modulation of acetylcholine levels most likely results in the control locomotory behavior and egg-laying (PubMed:12954868, PubMed:18614679).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | axon | |
Cellular Component | dendrite | |
Cellular Component | plasma membrane | |
Cellular Component | synapse | |
Molecular Function | G protein-coupled acetylcholine receptor activity | |
Molecular Function | G protein-coupled serotonin receptor activity | |
Biological Process | adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway | |
Biological Process | chemical synaptic transmission | |
Biological Process | G protein-coupled receptor signaling pathway | |
Biological Process | G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger | |
Biological Process | negative regulation of synaptic transmission, cholinergic | |
Biological Process | response to xenobiotic stimulus |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameMuscarinic acetylcholine receptor gar-2
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ09388
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Note: Diffusely localized in axons.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-9 | Extracellular | ||||
Sequence: MAVASVLLA | ||||||
Transmembrane | 10-30 | Helical; Name=1 | ||||
Sequence: LFMLFLSIVTVIGNLAVLLSY | ||||||
Topological domain | 31-41 | Cytoplasmic | ||||
Sequence: YLDKNIRQPTN | ||||||
Transmembrane | 42-62 | Helical; Name=2 | ||||
Sequence: YFIFSLAISDLLIGLEGIPVY | ||||||
Topological domain | 63-81 | Extracellular | ||||
Sequence: TAFYLNNNEWIWGDVLCDL | ||||||
Transmembrane | 82-102 | Helical; Name=3 | ||||
Sequence: WLSIDYIVCLASIYTVLGITV | ||||||
Topological domain | 103-122 | Cytoplasmic | ||||
Sequence: DRYYSVKKPATYRNWRTPGR | ||||||
Transmembrane | 123-143 | Helical; Name=4 | ||||
Sequence: VVLIIIFIWLVPSILFSVSIF | ||||||
Topological domain | 144-172 | Extracellular | ||||
Sequence: GYGTFTGTGRILKETECYVQFMTNPYLNM | ||||||
Transmembrane | 173-193 | Helical; Name=5 | ||||
Sequence: GMYISYYWTTLFVMLYLYWGI | ||||||
Topological domain | 194-549 | Cytoplasmic | ||||
Sequence: YRAAKKLALKSDQKTKRLALLTEMRRPEVSVRTSDAGNSSSDSPNDTSNSSKCFRTAPPTTTVQTTQTNVGTPPPVFRNHMTLHNNNMDFTKDNEIVRPPTPPDDNTYSNPNFSMISEQLTNGFSRQEPSSVIERESTAPCVSPEPSHASLENEFNENHHAHFKPELSLPFIDADSVSSMVGHDDLRRAMSIRISRSVSMQGTARATPVIEIVENLEEALKICENLEELREDENKNEEEKQKNGLENGGMNHVIIANDEQQPSTSKESEQKEEMTPENHDPNEVKVPLIAVSRVESVKSTAGGKVRRLITQMRSHSIRSKRKANKNKSVLSALNFFQRKKEYKSRSENRARKALRT | ||||||
Transmembrane | 550-570 | Helical; Name=6 | ||||
Sequence: ITFILGSFIILWTPFYVLATI | ||||||
Topological domain | 571-586 | Extracellular | ||||
Sequence: YGFCETCKASPSFNTL | ||||||
Transmembrane | 587-609 | Helical; Name=7 | ||||
Sequence: YTISYYLCYMNSPLNPFCYAMAN | ||||||
Topological domain | 610-627 | Cytoplasmic | ||||
Sequence: QQFKKTLTRIFKGDFRRV |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Egg-laying defect whereby 30% of eggs are laid at an early developmental stage (PubMed:12954868).
Increased sensitivity to the acetylcholine esterase inhibitor Aldicarb, which results in accelerated paralysis likely due to enhanced acetylcholine release by ventral cord neurons and enhanced depolarization of muscles on one side of the body (PubMed:18614679).
Exploratory behavior is similar to wild-type, but in contrast to wild-type, there is irregular locomotory behavior characterized by a 9.2% increase in speed of locomotion over a 1-minute interval, decreased turning frequency, reduced rate of reversals, and a 25% increase in the maximal distance covered over a 40 second interval (PubMed:18614679).
Double knockout with the G-protein coupled receptor for GABA subunit gbb-2 results in a slight increase in sensitivity to Aldicarb and accelerated paralysis 50 minutes following exposure to Aldicarb as compared to the gar-2 and gbb-2 single mutants (PubMed:18614679).
Increased sensitivity to the acetylcholine esterase inhibitor Aldicarb, which results in accelerated paralysis likely due to enhanced acetylcholine release by ventral cord neurons and enhanced depolarization of muscles on one side of the body (PubMed:18614679).
Exploratory behavior is similar to wild-type, but in contrast to wild-type, there is irregular locomotory behavior characterized by a 9.2% increase in speed of locomotion over a 1-minute interval, decreased turning frequency, reduced rate of reversals, and a 25% increase in the maximal distance covered over a 40 second interval (PubMed:18614679).
Double knockout with the G-protein coupled receptor for GABA subunit gbb-2 results in a slight increase in sensitivity to Aldicarb and accelerated paralysis 50 minutes following exposure to Aldicarb as compared to the gar-2 and gbb-2 single mutants (PubMed:18614679).
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000069049 | 1-627 | Muscarinic acetylcholine receptor gar-2 | |||
Sequence: MAVASVLLALFMLFLSIVTVIGNLAVLLSYYLDKNIRQPTNYFIFSLAISDLLIGLEGIPVYTAFYLNNNEWIWGDVLCDLWLSIDYIVCLASIYTVLGITVDRYYSVKKPATYRNWRTPGRVVLIIIFIWLVPSILFSVSIFGYGTFTGTGRILKETECYVQFMTNPYLNMGMYISYYWTTLFVMLYLYWGIYRAAKKLALKSDQKTKRLALLTEMRRPEVSVRTSDAGNSSSDSPNDTSNSSKCFRTAPPTTTVQTTQTNVGTPPPVFRNHMTLHNNNMDFTKDNEIVRPPTPPDDNTYSNPNFSMISEQLTNGFSRQEPSSVIERESTAPCVSPEPSHASLENEFNENHHAHFKPELSLPFIDADSVSSMVGHDDLRRAMSIRISRSVSMQGTARATPVIEIVENLEEALKICENLEELREDENKNEEEKQKNGLENGGMNHVIIANDEQQPSTSKESEQKEEMTPENHDPNEVKVPLIAVSRVESVKSTAGGKVRRLITQMRSHSIRSKRKANKNKSVLSALNFFQRKKEYKSRSENRARKALRTITFILGSFIILWTPFYVLATIYGFCETCKASPSFNTLYTISYYLCYMNSPLNPFCYAMANQQFKKTLTRIFKGDFRRV | ||||||
Disulfide bond | 79↔160 | |||||
Sequence: CDLWLSIDYIVCLASIYTVLGITVDRYYSVKKPATYRNWRTPGRVVLIIIFIWLVPSILFSVSIFGYGTFTGTGRILKETEC |
Keywords
- PTM
Proteomic databases
Expression
Tissue specificity
Expressed in putative sensory neurons, many cells of the ventral cord and in the HSN motor neurons (PubMed:11032868).
Expressed in some cholinergic motor neurons and GABAergic motor neurons, which are the two major types of ventral cord motor neurons (PubMed:18614679).
Expressed in some cholinergic motor neurons and GABAergic motor neurons, which are the two major types of ventral cord motor neurons (PubMed:18614679).
Developmental stage
Expressed throughout development from the embryo to the adult (PubMed:11032868, PubMed:11700045).
Expressed in the head region of larva (PubMed:11032868).
Expressed in the head region of larva (PubMed:11032868).
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 222-266 | Disordered | ||||
Sequence: VSVRTSDAGNSSSDSPNDTSNSSKCFRTAPPTTTVQTTQTNVGTP | ||||||
Compositional bias | 225-266 | Polar residues | ||||
Sequence: RTSDAGNSSSDSPNDTSNSSKCFRTAPPTTTVQTTQTNVGTP | ||||||
Compositional bias | 423-438 | Basic and acidic residues | ||||
Sequence: REDENKNEEEKQKNGL | ||||||
Region | 423-442 | Disordered | ||||
Sequence: REDENKNEEEKQKNGLENGG | ||||||
Region | 449-475 | Disordered | ||||
Sequence: ANDEQQPSTSKESEQKEEMTPENHDPN | ||||||
Compositional bias | 457-473 | Basic and acidic residues | ||||
Sequence: TSKESEQKEEMTPENHD |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Protein family/group databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q09388-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Namea
- Length627
- Mass (Da)71,483
- Last updated2002-06-06 v3
- ChecksumCB319E3BA8230AE5
Q09388-2
- Nameb
- Synonymsc
- Differences from canonical
- 520-532: Missing
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Features
Showing features for compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 225-266 | Polar residues | ||||
Sequence: RTSDAGNSSSDSPNDTSNSSKCFRTAPPTTTVQTTQTNVGTP | ||||||
Compositional bias | 423-438 | Basic and acidic residues | ||||
Sequence: REDENKNEEEKQKNGL | ||||||
Compositional bias | 457-473 | Basic and acidic residues | ||||
Sequence: TSKESEQKEEMTPENHD | ||||||
Alternative sequence | VSP_001862 | 520-532 | in isoform b | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF272738 EMBL· GenBank· DDBJ | AAK94896.1 EMBL· GenBank· DDBJ | mRNA | ||
AY053365 EMBL· GenBank· DDBJ | AAL15153.1 EMBL· GenBank· DDBJ | mRNA | ||
FO080618 EMBL· GenBank· DDBJ | CCD83363.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FO080618 EMBL· GenBank· DDBJ | CCD83364.1 EMBL· GenBank· DDBJ | Genomic DNA |