Q09388 · ACM2_CAEEL

  • Protein
    Muscarinic acetylcholine receptor gar-2
  • Gene
    gar-2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins (PubMed:11032868, PubMed:11700045).
Primary transducing effect is Pi turnover (By similarity).
Regulates the activity of ventral cord motor neurons (PubMed:18614679).
Couples to the G(o)-alpha G-protein subunit goa-1 to negatively regulate cholinergic receptor activity in the presence of high levels of the neurotransmitter acetylcholine in ventral cord motor neurons (PubMed:18614679).
As acetylcholine depolarizes body wall muscles, modulation of acetylcholine levels most likely results in the control locomotory behavior and egg-laying (PubMed:12954868, PubMed:18614679).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentaxon
Cellular Componentdendrite
Cellular Componentplasma membrane
Cellular Componentsynapse
Molecular FunctionG protein-coupled acetylcholine receptor activity
Molecular FunctionG protein-coupled serotonin receptor activity
Biological Processadenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway
Biological Processchemical synaptic transmission
Biological ProcessG protein-coupled receptor signaling pathway
Biological ProcessG protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
Biological Processnegative regulation of synaptic transmission, cholinergic
Biological Processresponse to xenobiotic stimulus

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Muscarinic acetylcholine receptor gar-2
  • Alternative names
    • G-protein-linked acetylcholine receptor 2

Gene names

    • Name
      gar-2
    • ORF names
      F47D12.1

Organism names

  • Taxonomic identifier
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    Q09388
  • Secondary accessions
    • Q09561
    • Q95WX7

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Cell projection, axon
Note: Diffusely localized in axons.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-9Extracellular
Transmembrane10-30Helical; Name=1
Topological domain31-41Cytoplasmic
Transmembrane42-62Helical; Name=2
Topological domain63-81Extracellular
Transmembrane82-102Helical; Name=3
Topological domain103-122Cytoplasmic
Transmembrane123-143Helical; Name=4
Topological domain144-172Extracellular
Transmembrane173-193Helical; Name=5
Topological domain194-549Cytoplasmic
Transmembrane550-570Helical; Name=6
Topological domain571-586Extracellular
Transmembrane587-609Helical; Name=7
Topological domain610-627Cytoplasmic

Keywords

Phenotypes & Variants

Disruption phenotype

Egg-laying defect whereby 30% of eggs are laid at an early developmental stage (PubMed:12954868).
Increased sensitivity to the acetylcholine esterase inhibitor Aldicarb, which results in accelerated paralysis likely due to enhanced acetylcholine release by ventral cord neurons and enhanced depolarization of muscles on one side of the body (PubMed:18614679).
Exploratory behavior is similar to wild-type, but in contrast to wild-type, there is irregular locomotory behavior characterized by a 9.2% increase in speed of locomotion over a 1-minute interval, decreased turning frequency, reduced rate of reversals, and a 25% increase in the maximal distance covered over a 40 second interval (PubMed:18614679).
Double knockout with the G-protein coupled receptor for GABA subunit gbb-2 results in a slight increase in sensitivity to Aldicarb and accelerated paralysis 50 minutes following exposure to Aldicarb as compared to the gar-2 and gbb-2 single mutants (PubMed:18614679).

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00000690491-627Muscarinic acetylcholine receptor gar-2
Disulfide bond79↔160

Keywords

Proteomic databases

Expression

Tissue specificity

Expressed in putative sensory neurons, many cells of the ventral cord and in the HSN motor neurons (PubMed:11032868).
Expressed in some cholinergic motor neurons and GABAergic motor neurons, which are the two major types of ventral cord motor neurons (PubMed:18614679).

Developmental stage

Expressed throughout development from the embryo to the adult (PubMed:11032868, PubMed:11700045).
Expressed in the head region of larva (PubMed:11032868).

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region222-266Disordered
Compositional bias225-266Polar residues
Compositional bias423-438Basic and acidic residues
Region423-442Disordered
Region449-475Disordered
Compositional bias457-473Basic and acidic residues

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Protein family/group databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q09388-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    627
  • Mass (Da)
    71,483
  • Last updated
    2002-06-06 v3
  • Checksum
    CB319E3BA8230AE5
MAVASVLLALFMLFLSIVTVIGNLAVLLSYYLDKNIRQPTNYFIFSLAISDLLIGLEGIPVYTAFYLNNNEWIWGDVLCDLWLSIDYIVCLASIYTVLGITVDRYYSVKKPATYRNWRTPGRVVLIIIFIWLVPSILFSVSIFGYGTFTGTGRILKETECYVQFMTNPYLNMGMYISYYWTTLFVMLYLYWGIYRAAKKLALKSDQKTKRLALLTEMRRPEVSVRTSDAGNSSSDSPNDTSNSSKCFRTAPPTTTVQTTQTNVGTPPPVFRNHMTLHNNNMDFTKDNEIVRPPTPPDDNTYSNPNFSMISEQLTNGFSRQEPSSVIERESTAPCVSPEPSHASLENEFNENHHAHFKPELSLPFIDADSVSSMVGHDDLRRAMSIRISRSVSMQGTARATPVIEIVENLEEALKICENLEELREDENKNEEEKQKNGLENGGMNHVIIANDEQQPSTSKESEQKEEMTPENHDPNEVKVPLIAVSRVESVKSTAGGKVRRLITQMRSHSIRSKRKANKNKSVLSALNFFQRKKEYKSRSENRARKALRTITFILGSFIILWTPFYVLATIYGFCETCKASPSFNTLYTISYYLCYMNSPLNPFCYAMANQQFKKTLTRIFKGDFRRV

Q09388-2

  • Name
    b
  • Synonyms
    c
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
Q8MQ40Q8MQ40_CAEELgar-2245
Q8MQ41Q8MQ41_CAEELgar-2664
Q8I7J8Q8I7J8_CAEELgar-2334

Features

Showing features for compositional bias, alternative sequence.

TypeIDPosition(s)Description
Compositional bias225-266Polar residues
Compositional bias423-438Basic and acidic residues
Compositional bias457-473Basic and acidic residues
Alternative sequenceVSP_001862520-532in isoform b

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF272738
EMBL· GenBank· DDBJ
AAK94896.1
EMBL· GenBank· DDBJ
mRNA
AY053365
EMBL· GenBank· DDBJ
AAL15153.1
EMBL· GenBank· DDBJ
mRNA
FO080618
EMBL· GenBank· DDBJ
CCD83363.1
EMBL· GenBank· DDBJ
Genomic DNA
FO080618
EMBL· GenBank· DDBJ
CCD83364.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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