Q08834 · MGT5A_RAT

  • Protein
    Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
  • Gene
    Mgat5
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides (PubMed:7615638, PubMed:8340368).
Catalyzes an important step in the biosynthesis of branched, complex-type N-glycans, such as those found on EGFR, TGFR (TGF-beta receptor) and CDH2 (By similarity).
Via its role in the biosynthesis of complex N-glycans, plays an important role in the activation of cellular signaling pathways, reorganization of the actin cytoskeleton, cell-cell adhesion and cell migration (PubMed:7615638).
MGAT5-dependent EGFR N-glycosylation enhances the interaction between EGFR and LGALS3 and thereby prevents rapid EGFR endocytosis and prolongs EGFR signaling. Required for efficient interaction between TGFB1 and its receptor. Enhances activation of intracellular signaling pathways by several types of growth factors, including FGF2, PDGF, IGF, TGFB1 and EGF. MGAT5-dependent CDH2 N-glycosylation inhibits CDH2-mediated homotypic cell-cell adhesion and contributes to the regulation of downstream signaling pathways. Promotes cell migration. Contributes to the regulation of the inflammatory response. MGAT5-dependent TCR N-glycosylation enhances the interaction between TCR and LGALS3, limits agonist-induced TCR clustering, and thereby dampens TCR-mediated responses to antigens. Required for normal leukocyte evasation and accumulation at sites of inflammation (By similarity).
Inhibits attachment of monocytes to the vascular endothelium and subsequent monocyte diapedesis (By similarity).

Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

Promotes proliferation of umbilical vein endothelial cells and angiogenesis, at least in part by promoting the release of the growth factor FGF2 from the extracellular matrix.

Pathway

Protein modification; protein glycosylation.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site377-378substrate
Binding site525UDP-N-acetyl-alpha-D-glucosamine (UniProtKB | ChEBI)
Binding site553substrate

GO annotations

AspectTerm
Cellular Componentextracellular region
Cellular ComponentGolgi apparatus
Cellular ComponentGolgi membrane
Cellular Componentmembrane
Molecular Functionacetylglucosaminyltransferase activity
Molecular Functionalpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity
Molecular Functionglycosyltransferase activity
Molecular Functionmanganese ion binding
Molecular Functionprotein phosphatase inhibitor activity
Biological Processpositive regulation of cell migration
Biological Processpositive regulation of receptor signaling pathway via STAT
Biological Processprotein N-linked glycosylation
Biological Processprotein N-linked glycosylation via asparagine

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
  • EC number
  • Alternative names
    • Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
    • GlcNAc-T V
      (GNT-V)
    • Mannoside acetylglucosaminyltransferase 5
    • N-acetylglucosaminyl-transferase V
  • Cleaved into 1 chains

Gene names

    • Name
      Mgat5

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    Q08834

Proteomes

Organism-specific databases

Subcellular Location

Golgi apparatus membrane
; Single-pass type II membrane protein

Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

Secreted

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-13Cytoplasmic
Transmembrane14-30Helical; Signal-anchor for type II membrane protein
Topological domain31-740Lumenal

Keywords

Phenotypes & Variants

Variants

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The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Chemistry

PTM/Processing

Features

Showing features for chain, glycosylation, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00000805241-740Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
ChainPRO_000044569431-740Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
Glycosylation109N-linked (GlcNAc...) asparagine
Glycosylation114N-linked (GlcNAc...) asparagine
Glycosylation117N-linked (GlcNAc...) asparagine
Disulfide bond144↔182
Disulfide bond155↔195
Disulfide bond171↔337
Glycosylation333N-linked (GlcNAc...) asparagine
Disulfide bond371↔625
Glycosylation432N-linked (GlcNAc...) asparagine
Glycosylation446N-linked (GlcNAc...) asparagine
Disulfide bond648↔723
Disulfide bond652↔725
Disulfide bond659↔712
Disulfide bond680↔701
Disulfide bond736↔739

Post-translational modification

N-glycosylated.
A secreted form is released from the membrane after cleavage by gamma-secretase.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Detected in kidney (at protein level). Detected in kidney.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region212-740Sufficient for catalytic activity

Sequence similarities

Belongs to the glycosyltransferase 18 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    740
  • Mass (Da)
    84,562
  • Last updated
    1995-02-01 v1
  • Checksum
    C0BD7F820FEA959C
MAFFSPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQPESSSMLREQILDLSKRYIKALAEENRNVVDGPYAGVMTAYDLKKTLAVLLDNILQRIGKLESKVDNLVNGTGANSTNSTTAVPSLVSLEKINVADIINGVQEKCVLPPMDGYPHCEGKIKWMKDMWRSDPCYADYGVDGTSCSFFIYLSEVENWCPRLPWRAKNPYEEADHNSLAEIRTDFNILYGMMKKHEEFRWMRLRIRRMADAWIQAIKSLAEKQNLEKRKRKKILVHLGLLTKESGFKIAETAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKEIMKKVVGNRSGCPTVGDRIVELIYIDIVGLAQFKKTLGPSWVHYQCMLRVLDSFGTEPEFNHASYAQSKGHKTPWGKWNLNPQQFYTMFPHTPDNSFLGFVVEQHLNSSDIHHINEIKRQNQSLVYGKVDSFWKNKKIYLDIIHTYMEVHATVYGSSTKNIPSYVKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRELTSQHPYAEVFIGRPHVWTVDLNNREEVEDAVKAILNQKIEPYMPYEFTCEGMLQRINAFIEKQDFCHGQVMWPPLSALQVKLAEPGQSCKQVCQESQLICEPSFFQHLNKEKDLLKYKVICQSSELYKDILVPSFYPKSKHCVFQGDLLLFSCAGAHPTHQRICPCRDFIKGQVALCKDCL

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8I5Y5S9A0A8I5Y5S9_RATMgat5741

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L14284
EMBL· GenBank· DDBJ
AAA41665.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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