Q08352 · DHA_BACSU
- ProteinAlanine dehydrogenase
- Geneald
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids378 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the reversible oxidative deamination of L-alanine to pyruvate. Oxidative deamination proceeds through a sequential, ordered ternary-binary mechanism, where NAD+ binds first followed by L-alanine; the products are released in the order ammonia, pyruvate and NADH (Probable) (PubMed:6794611, PubMed:6794612).
Disruption blocks sporulation probably in stage V; 20-30% sporulation can be restored if the media is supplemented with pyruvate, suggesting lack of pyruvate blocks sporulation. Thus it is a key factor in the assimilation of L-alanine as an energy source via the tricarboxylic acid cycle during sporulation (PubMed:8226620).
Disruption blocks sporulation probably in stage V; 20-30% sporulation can be restored if the media is supplemented with pyruvate, suggesting lack of pyruvate blocks sporulation. Thus it is a key factor in the assimilation of L-alanine as an energy source via the tricarboxylic acid cycle during sporulation (PubMed:8226620).
Catalytic activity
- H2O + L-alanine + NAD+ = H+ + NADH + NH4+ + pyruvateThis reaction proceeds in the forward and the backward directions.
Pathway
Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH3 and pyruvate from L-alanine: step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 15 | substrate | ||||
Sequence: R | ||||||
Binding site | 74 | substrate | ||||
Sequence: K | ||||||
Active site | 95 | Proton donor/acceptor | ||||
Sequence: H | ||||||
Binding site | 132 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 176-177 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: VV | ||||||
Binding site | 196 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 218 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 237-238 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: VL | ||||||
Binding site | 265-268 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: VAID | ||||||
Active site | 268 | Proton donor/acceptor | ||||
Sequence: D | ||||||
Binding site | 297-300 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: VANM |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | alanine dehydrogenase activity | |
Molecular Function | nucleotide binding | |
Biological Process | alanine catabolic process | |
Biological Process | L-alanine catabolic process | |
Biological Process | sporulation resulting in formation of a cellular spore |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAlanine dehydrogenase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionQ08352
Proteomes
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Strain cannot grow on L-alanine as a sole carbon source. Disruption of ald causes a sporulation defect, most likely in stage V.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000198992 | 1-378 | Alanine dehydrogenase | |||
Sequence: MIIGVPKEIKNNENRVALTPGGVSQLISNGHRVLVETGAGLGSGFENEAYESAGAEIIADPKQVWDAEMVMKVKEPLPEEYVYFRKGLVLFTYLHLAAEPELAQALKDKGVTAIAYETVSEGRTLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLAGVPGVSRGKVTIIGGGVVGTNAAKMAVGLGADVTIIDLNADRLRQLDDIFGHQIKTLISNPVNIADAVAEADLLICAVLIPGAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDQPTYEKHGVVHYAVANMPGAVPRTSTIALTNVTVPYALQIANKGAVKALADNTALRAGLNTANGHVTYEAVARDLGYEYVPAEKALQDESSVAGA |
Proteomic databases
Expression
Induction
Interaction
Structure
Sequence
- Sequence statusComplete
- Length378
- Mass (Da)39,684
- Last updated1994-10-01 v1
- ChecksumB5D6113047FCF211
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L20916 EMBL· GenBank· DDBJ | AAA16038.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z82015 EMBL· GenBank· DDBJ | CAB04775.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL009126 EMBL· GenBank· DDBJ | CAB15181.1 EMBL· GenBank· DDBJ | Genomic DNA |