Q08352 · DHA_BACSU

Function

function

Catalyzes the reversible oxidative deamination of L-alanine to pyruvate. Oxidative deamination proceeds through a sequential, ordered ternary-binary mechanism, where NAD+ binds first followed by L-alanine; the products are released in the order ammonia, pyruvate and NADH (Probable) (PubMed:6794611, PubMed:6794612).
Disruption blocks sporulation probably in stage V; 20-30% sporulation can be restored if the media is supplemented with pyruvate, suggesting lack of pyruvate blocks sporulation. Thus it is a key factor in the assimilation of L-alanine as an energy source via the tricarboxylic acid cycle during sporulation (PubMed:8226620).

Catalytic activity

Pathway

Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH3 and pyruvate from L-alanine: step 1/1.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site15substrate
Binding site74substrate
Active site95Proton donor/acceptor
Binding site132NAD+ (UniProtKB | ChEBI)
Binding site176-177NAD+ (UniProtKB | ChEBI)
Binding site196NAD+ (UniProtKB | ChEBI)
Binding site218NAD+ (UniProtKB | ChEBI)
Binding site237-238NAD+ (UniProtKB | ChEBI)
Binding site265-268NAD+ (UniProtKB | ChEBI)
Active site268Proton donor/acceptor
Binding site297-300NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionalanine dehydrogenase activity
Molecular Functionnucleotide binding
Biological Processalanine catabolic process
Biological ProcessL-alanine catabolic process
Biological Processsporulation resulting in formation of a cellular spore

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Alanine dehydrogenase
  • EC number
  • Alternative names
    • Stage V sporulation protein N

Gene names

    • Name
      ald
    • Synonyms
      ski22
      , spoVN
    • Ordered locus names
      BSU31930

Organism names

Accessions

  • Primary accession
    Q08352

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Strain cannot grow on L-alanine as a sole carbon source. Disruption of ald causes a sporulation defect, most likely in stage V.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001989921-378Alanine dehydrogenase

Proteomic databases

Expression

Induction

A monocistronic operon. Induced by L-alanine in minimal media. Constitutively expressed in rich medium, declines during sporulation. Induced early during sporulation on minimal medium by L-alanine. Affects its own expression (PubMed:8226620).
Transcription activated by AdeR (PubMed:22797752).

Interaction

Subunit

Homohexamer. Trimer of dimer (By similarity).

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the AlaDH/PNT family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    378
  • Mass (Da)
    39,684
  • Last updated
    1994-10-01 v1
  • Checksum
    B5D6113047FCF211
MIIGVPKEIKNNENRVALTPGGVSQLISNGHRVLVETGAGLGSGFENEAYESAGAEIIADPKQVWDAEMVMKVKEPLPEEYVYFRKGLVLFTYLHLAAEPELAQALKDKGVTAIAYETVSEGRTLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLAGVPGVSRGKVTIIGGGVVGTNAAKMAVGLGADVTIIDLNADRLRQLDDIFGHQIKTLISNPVNIADAVAEADLLICAVLIPGAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHITTHDQPTYEKHGVVHYAVANMPGAVPRTSTIALTNVTVPYALQIANKGAVKALADNTALRAGLNTANGHVTYEAVARDLGYEYVPAEKALQDESSVAGA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L20916
EMBL· GenBank· DDBJ
AAA16038.1
EMBL· GenBank· DDBJ
Genomic DNA
Z82015
EMBL· GenBank· DDBJ
CAB04775.1
EMBL· GenBank· DDBJ
Genomic DNA
AL009126
EMBL· GenBank· DDBJ
CAB15181.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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