Q08271 · GAS4_YEAST
- Protein1,3-beta-glucanosyltransferase GAS4
- GeneGAS4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids471 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. Involved in spore wall assembly.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 88 | (1,3-beta-D-glucosyl)n 1 (UniProtKB | ChEBI); donor substrate | |||
Binding site | 160 | (1,3-beta-D-glucosyl)n 1 (UniProtKB | ChEBI); donor substrate | |||
Active site | 161 | Proton donor | |||
Binding site | 161 | (1,3-beta-D-glucosyl)n 2 (UniProtKB | ChEBI); acceptor substrate | |||
Binding site | 203 | (1,3-beta-D-glucosyl)n 2 (UniProtKB | ChEBI); acceptor substrate | |||
Binding site | 208 | (1,3-beta-D-glucosyl)n 2 (UniProtKB | ChEBI); acceptor substrate | |||
Active site | 266 | Nucleophile | |||
Binding site | 298 | (1,3-beta-D-glucosyl)n 1 (UniProtKB | ChEBI); donor substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | fungal-type cell wall | |
Cellular Component | fungal-type vacuole | |
Cellular Component | plasma membrane | |
Cellular Component | side of membrane | |
Molecular Function | 1,3-beta-glucanosyltransferase activity | |
Biological Process | ascospore wall assembly | |
Biological Process | fungal-type cell wall (1->3)-beta-D-glucan biosynthetic process | |
Biological Process | fungal-type cell wall organization |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended name1,3-beta-glucanosyltransferase GAS4
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ08271
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, disulfide bond, glycosylation, lipidation, propeptide.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-21 | ||||
Chain | PRO_0000010479 | 22-447 | 1,3-beta-glucanosyltransferase GAS4 | ||
Disulfide bond | 70↔99 | ||||
Glycosylation | 151 | N-linked (GlcNAc...) asparagine | |||
Disulfide bond | 217↔354 | ||||
Disulfide bond | 238↔269 | ||||
Glycosylation | 398 | N-linked (GlcNAc...) asparagine | |||
Lipidation | 447 | GPI-anchor amidated asparagine | |||
Propeptide | PRO_0000010480 | 448-471 | Removed in mature form | ||
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Developmental stage
Expressed exclusively during sporulation (at protein level).
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length471
- Mass (Da)53,813
- Last updated1997-11-01 v1
- MD5 ChecksumE9F6ACF2151623DAA407909142A9A6A5
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X95465 EMBL· GenBank· DDBJ | CAA64738.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z74874 EMBL· GenBank· DDBJ | CAA99152.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006948 EMBL· GenBank· DDBJ | DAA10652.1 EMBL· GenBank· DDBJ | Genomic DNA |