Q07648 · DTD_YEAST
- ProteinD-aminoacyl-tRNA deacylase
- GeneDTD1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids150 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs (Probable). Hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr) (PubMed:10766779).
May also deacylate mischarged D-leucyl-tRNA(Leu) (PubMed:10918062).
Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS (By similarity).
Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site (By similarity).
By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality (By similarity).
May also deacylate mischarged D-leucyl-tRNA(Leu) (PubMed:10918062).
Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS (By similarity).
Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site (By similarity).
By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality (By similarity).
Miscellaneous
Present with 4610 molecules/cell in log phase SD medium.
Catalytic activity
- glycyl-tRNA(Ala) + H2O = glycine + H+ + tRNA(Ala)
- D-tyrosyl-tRNA(Tyr) + H2O = D-tyrosine + tRNA(Tyr)
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | D-leucyl-tRNA(Leu) deacylase activity | |
Molecular Function | D-tyrosyl-tRNA(Tyr) deacylase activity | |
Molecular Function | tRNA binding | |
Biological Process | D-leucine catabolic process | |
Biological Process | D-tyrosine catabolic process | |
Biological Process | tRNA metabolic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameD-aminoacyl-tRNA deacylase
- EC number
- Short namesDTD
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ07648
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
10-fold decrease in D-tyrosyl-tRNA(Tyr) deacylase activity, growth becomes more sensitive to D-tyrosine and is inhibited at 0.1 mM D-Tyr (PubMed:10766779, PubMed:10918062).
Growth is also inhibited in the presence of 0.3 mM D-leucine; wild-type cells grow well in up to 0.3 mM D-Tyr and 10 mM D-Leu (PubMed:10918062).
No growth differences observed in the presence of the other D-amino acids (PubMed:10918062).
Growth is also inhibited in the presence of 0.3 mM D-leucine; wild-type cells grow well in up to 0.3 mM D-Tyr and 10 mM D-Leu (PubMed:10918062).
No growth differences observed in the presence of the other D-amino acids (PubMed:10918062).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000164634 | 1-150 | D-aminoacyl-tRNA deacylase | |||
Sequence: MKIVLQKVSQASVVVDSKVISSIKHGYMLLVGISIDDSMAEIDKLSKKVLSLRIFEDESRNLWKKNIKEANGEILSVSQFTLMAKTKKGTKPDFHLAQKGHIAKELYEEFLKLLRSDLGEEKVKDGEFGAMMSCSLTNEGPVTIILDSDQ |
Proteomic databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 140-141 | Gly-cisPro motif, important for rejection of L-amino acids | ||||
Sequence: GP |
Domain
A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.
Sequence similarities
Belongs to the DTD family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length150
- Mass (Da)16,746
- Last updated1996-11-01 v1
- Checksum7773D8A1FBA5E982
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z74267 EMBL· GenBank· DDBJ | CAA98798.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006938 EMBL· GenBank· DDBJ | DAA11645.1 EMBL· GenBank· DDBJ | Genomic DNA |