Q07496 · EPHA4_CHICK

  • Protein
    Ephrin type-A receptor 4
  • Gene
    EPHA4
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections (By similarity).

Catalytic activity

Features

Showing features for binding site, active site.

1986100200300400500600700800900
TypeIDPosition(s)Description
Binding site627-635ATP (UniProtKB | ChEBI)
Binding site653ATP (UniProtKB | ChEBI)
Active site746Proton acceptor

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentdendrite
Cellular Componentearly endosome membrane
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular FunctionDH domain binding
Molecular FunctionGPI-linked ephrin receptor activity
Molecular Functionprotein kinase activity
Molecular Functiontransmembrane-ephrin receptor activity
Biological Processaxon guidance
Biological Processcell adhesion
Biological Processephrin receptor signaling pathway
Biological Processfasciculation of sensory neuron axon
Biological Processpeptidyl-tyrosine phosphorylation
Biological Processpositive regulation of Rho guanyl-nucleotide exchange factor activity
Biological Processprotein autophosphorylation
Biological Processregulation of GTPase activity
Biological Processsegmentation
Biological Processsomitogenesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ephrin type-A receptor 4
  • EC number
  • Alternative names
    • EPH-like kinase 8 (EK8; cEK8)

Gene names

    • Name
      EPHA4
    • Synonyms
      CEK8

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Galloanserae > Galliformes > Phasianidae > Phasianinae > Gallus

Accessions

  • Primary accession
    Q07496
  • Secondary accessions
    • Q90772

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Single-pass type I membrane protein
Early endosome
Note: Clustered upon activation and targeted to early endosome.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain20-547Extracellular
Transmembrane548-569Helical
Topological domain570-986Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for signal, chain, glycosylation, modified residue.

TypeIDPosition(s)Description
Signal1-19
ChainPRO_000001680920-986Ephrin type-A receptor 4
Glycosylation235N-linked (GlcNAc...) asparagine
Glycosylation340N-linked (GlcNAc...) asparagine
Glycosylation408N-linked (GlcNAc...) asparagine
Modified residue596Phosphotyrosine; by autocatalysis
Modified residue602Phosphotyrosine; by autocatalysis
Modified residue779Phosphotyrosine; by autocatalysis
Modified residue928Phosphotyrosine; by autocatalysis

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed at high levels in brain, with expression also detected in the kidney, lung, muscle and thymus.

Gene expression databases

Interaction

Subunit

Interacts with the src family kinase, p59-Fyn, through the major phosphorylation site at position Tyr-602 (By similarity).
Interacts (via PDZ motif) with SIPA1L1 (via PDZ domain); controls neuronal morphology through regulation of the RAP1 (RAP1A or RAP1B) and RAP2 (RAP2A, RAP2B or RAP2C) GTPases

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain30-209Eph LBD
Domain328-439Fibronectin type-III 1
Domain440-537Fibronectin type-III 2
Domain621-882Protein kinase
Domain911-975SAM
Motif984-986PDZ-binding

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    986
  • Mass (Da)
    109,483
  • Last updated
    1997-11-01 v2
  • Checksum
    BD88C2A5BD840A0F
MAGVPVGALLPLLVGVCGAVTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEPSQNNWLRTDWIPREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESNNDKERFIRESQFAKIDTIAADESFTQVDIGDRIMKLNTEVRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGYEERNGECQACKIGYYKALSTDVACAKCPPHSYSIWEGSTSCTCDRGFFRAENDAASMPCTRPPSAPQNLISNVNETSVNLEWSAPQNKGGRDDISYNVVCKRCGAGEPSHCRSCGSGVHFSPQQNGLKTTKVSITDLLAHTNYTFEVWAVNGVSKHNPSQDQAVSVTVTTNQAAPSPIALIQAKEITRHSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVKTASRNTDIKGLNPLTSYVFHVRARTAAGYGDFSGPFEFTTNTVPSPIIGDGTNPTVLLVSVAGSVVLVVILIAAFVISRRRSKYSKAKQEADEEKHLNQGVRTYVDPFTYEDPNQAVREFAKEIDASCIKIEKVIGVGEFGEVCSGRLKVPGKREICVAIKTLKAGYTDKQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITEYMENGSLDAFLRKNDGRFTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGMSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPPPMDCPIALHQLMLDCWQKERSDRPKFGQIVNMLDKLIRNPNSLKRTGSESSRPSTALLDPSSPEFSAVVSVSDWLQAIKMERYKDNFTAAGYTTLEAVVHMNQDDLARIGITAITHQNKILSSVQAMRSQMQQMHGRMVPV

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict138in Ref. 2; CAA79509
Sequence conflict487in Ref. 2; CAA79509

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D38174
EMBL· GenBank· DDBJ
BAA07373.1
EMBL· GenBank· DDBJ
mRNA
Z19059
EMBL· GenBank· DDBJ
CAA79509.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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