Q07292 · KRAF1_CAEEL
- ProteinRaf homolog serine/threonine-protein kinase
- Genelin-45
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids813 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Protein kinase that participates in the induction of vulva and has roles in fertility and viability. Acts downstream of the Ras protein let-60 (PubMed:11861555, PubMed:14724126, PubMed:8483497).
Required for progression of developing oocytes through the pachytene stage (PubMed:19826475).
Plays a role in responses to M.nematophilum-mediated bacterial infection by promoting tail swelling and preventing constipation (PubMed:15268855).
Positively regulates lifespan upstream of mek-2 and mpk-1 (PubMed:20624915).
Required for progression of developing oocytes through the pachytene stage (PubMed:19826475).
Plays a role in responses to M.nematophilum-mediated bacterial infection by promoting tail swelling and preventing constipation (PubMed:15268855).
Positively regulates lifespan upstream of mek-2 and mpk-1 (PubMed:20624915).
Miscellaneous
Gld-1 binds to the 3'-UTR of lin-45 mRNA and is able to protect nonsense-containing lin-45 mRNA from nonsense mediated decay (NMD).
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Cofactor
Note: Binds 2 Zn2+ ions per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 184 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 187 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 198 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 201 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 206 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 209 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 217 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 487-495 | ATP (UniProtKB | ChEBI) | ||||
Sequence: VGSGSFGTV | ||||||
Binding site | 507 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Active site | 602 | Proton acceptor | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | MAP kinase kinase kinase activity | |
Molecular Function | metal ion binding | |
Molecular Function | protein serine kinase activity | |
Molecular Function | small GTPase binding | |
Biological Process | cell fate specification | |
Biological Process | defense response to Gram-positive bacterium | |
Biological Process | MAPK cascade | |
Biological Process | meiotic cell cycle | |
Biological Process | nematode larval development | |
Biological Process | oogenesis | |
Biological Process | Ras protein signal transduction | |
Biological Process | reproductive process | |
Biological Process | vulval development |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRaf homolog serine/threonine-protein kinase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ07292
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
RNAi-mediated knockdown causes a defect in pachytene progression resulting in a proximal gonad devoid of nuclei. The phenotype is more severe in gck-1 (km15) mutant (PubMed:19826475).
RNAi-mediated knockdown in adults decreases lifespan (PubMed:20624915).
RNAi-mediated knockdown in adults decreases lifespan (PubMed:20624915).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 92 | In n2018; 76% larval lethal, 24% abnormal vulval development. | ||||
Sequence: P → S | ||||||
Mutagenesis | 108 | In n1925; no effect. | ||||
Sequence: R → W | ||||||
Mutagenesis | 118 | In n2506; 86% larval lethal, 93% abnormal vulval development. | ||||
Sequence: R → W | ||||||
Mutagenesis | 312 | Probably abolishes phosphorylation. Multivulva formation. Multivulva formation; when associated with A-453. | ||||
Sequence: S → A | ||||||
Mutagenesis | 453 | Probably abolishes phosphorylation. Multivulva formation; when associated with A-312. | ||||
Sequence: S → A | ||||||
Mutagenesis | 645 | In oz178 and oz201; 55% larval lethal, 100% abnormal vulval development. | ||||
Sequence: S → N | ||||||
Mutagenesis | 726 | In n1924; 5% larval lethal, no effect on vulval development. | ||||
Sequence: I → F | ||||||
Mutagenesis | 754 | In n2520 and n2523; no effect. | ||||
Sequence: S → F |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000086193 | 1-813 | Raf homolog serine/threonine-protein kinase | |||
Sequence: MSRINFKKSSASTTPTSPHCPSPRLISLPRCASSSIDRKDQASPMASPSTPLYPKHSDSLHSLSGHHSAGGAGTSDKEPPKFKYKMIMVHLPFDQHSRVEVRPGETARDAISKLLKKRNITPQLCHVNASSDPKQESIELSLTMEEIASRLPGNELWVHSEYLNTVSSIKHAIVRRTFIPPKSCDVCNNPIWMMGFRCEFCQFKFHQRCSSFAPLYCDLLQSVPKNEDLVKELFGIASQVEGPDRSVAEIVLANLAPTSGQSPAATPDSSHPDLTSIKRTGGVKRHPMAVSPQNETSQLSPSGPYPRDRSSSAPNINAINDEATVQHNQRILDALEAQRLEEESRDKTGSLLSTQARHRPHFQSGHILSGARMNRLHPLVDCTPLGSNSPSSTCSSPPGGLIGQPTLGQSPNVSGSTTSSLVAAHLHTLPLTPPQSAPPQKISPGFFRNRSRSPGERLDAQRPRPPQKPHHEDWEILPNEFIIQYKVGSGSFGTVYRGEFFGTVAIKKLNVVDPTPSQMAAFKNEVAVLKKTRHLNVLLFMGWVREPEIAIITQWCEGSSLYRHIHVQEPRVEFEMGAIIDILKQVSLGMNYLHSKNIIHRDLKTNNIFLMDDMSTVKIGDFGLATVKTKWTVNGGQQQQQPTGSILWMAPEVIRMQDDNPYTPQSDVYSFGICMYEILSSHLPYSNINNRDQILFMVGRGYLRPDRSKIRHDTPKSMLKLYDNCIMFDRNERPVFGEVLERLRDIILPKLTRSQSAPNVLHLDSQYSVMDAVMRSQMLSWSYIPPATAKTPQSAAAAAAANKKAYYNVYGLI |
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain, zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-21 | Polar residues | ||||
Sequence: MSRINFKKSSASTTPTSPHCP | ||||||
Region | 1-79 | Disordered | ||||
Sequence: MSRINFKKSSASTTPTSPHCPSPRLISLPRCASSSIDRKDQASPMASPSTPLYPKHSDSLHSLSGHHSAGGAGTSDKEP | ||||||
Compositional bias | 34-57 | Polar residues | ||||
Sequence: SSIDRKDQASPMASPSTPLYPKHS | ||||||
Domain | 85-161 | RBD | ||||
Sequence: KMIMVHLPFDQHSRVEVRPGETARDAISKLLKKRNITPQLCHVNASSDPKQESIELSLTMEEIASRLPGNELWVHSE | ||||||
Zinc finger | 170-217 | Phorbol-ester/DAG-type | ||||
Sequence: KHAIVRRTFIPPKSCDVCNNPIWMMGFRCEFCQFKFHQRCSSFAPLYC | ||||||
Compositional bias | 258-276 | Polar residues | ||||
Sequence: TSGQSPAATPDSSHPDLTS | ||||||
Region | 258-315 | Disordered | ||||
Sequence: TSGQSPAATPDSSHPDLTSIKRTGGVKRHPMAVSPQNETSQLSPSGPYPRDRSSSAPN | ||||||
Compositional bias | 290-315 | Polar residues | ||||
Sequence: VSPQNETSQLSPSGPYPRDRSSSAPN | ||||||
Region | 338-358 | Disordered | ||||
Sequence: QRLEEESRDKTGSLLSTQARH | ||||||
Region | 383-472 | Disordered | ||||
Sequence: TPLGSNSPSSTCSSPPGGLIGQPTLGQSPNVSGSTTSSLVAAHLHTLPLTPPQSAPPQKISPGFFRNRSRSPGERLDAQRPRPPQKPHHE | ||||||
Compositional bias | 384-425 | Polar residues | ||||
Sequence: PLGSNSPSSTCSSPPGGLIGQPTLGQSPNVSGSTTSSLVAAH | ||||||
Compositional bias | 452-472 | Basic and acidic residues | ||||
Sequence: RSPGERLDAQRPRPPQKPHHE | ||||||
Domain | 481-748 | Protein kinase | ||||
Sequence: FIIQYKVGSGSFGTVYRGEFFGTVAIKKLNVVDPTPSQMAAFKNEVAVLKKTRHLNVLLFMGWVREPEIAIITQWCEGSSLYRHIHVQEPRVEFEMGAIIDILKQVSLGMNYLHSKNIIHRDLKTNNIFLMDDMSTVKIGDFGLATVKTKWTVNGGQQQQQPTGSILWMAPEVIRMQDDNPYTPQSDVYSFGICMYEILSSHLPYSNINNRDQILFMVGRGYLRPDRSKIRHDTPKSMLKLYDNCIMFDRNERPVFGEVLERLRDIIL |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
Q07292-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Namea
- Length813
- Mass (Da)90,407
- Last updated2002-07-11 v2
- Checksum6376E968D11A9E49
Q07292-2
- Nameb
Q07292-3
- Namec
- Differences from canonical
- 1-44: Missing
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Features
Showing features for alternative sequence, compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_020620 | 1 | in isoform b | |||
Sequence: M → MSAMAVLYSVCHFHGGWNRRKSAPKSPVTSSQTPSSSSTRRRM | ||||||
Compositional bias | 1-21 | Polar residues | ||||
Sequence: MSRINFKKSSASTTPTSPHCP | ||||||
Alternative sequence | VSP_020621 | 1-44 | in isoform c | |||
Sequence: Missing | ||||||
Compositional bias | 34-57 | Polar residues | ||||
Sequence: SSIDRKDQASPMASPSTPLYPKHS | ||||||
Compositional bias | 258-276 | Polar residues | ||||
Sequence: TSGQSPAATPDSSHPDLTS | ||||||
Compositional bias | 290-315 | Polar residues | ||||
Sequence: VSPQNETSQLSPSGPYPRDRSSSAPN | ||||||
Compositional bias | 384-425 | Polar residues | ||||
Sequence: PLGSNSPSSTCSSPPGGLIGQPTLGQSPNVSGSTTSSLVAAH | ||||||
Compositional bias | 452-472 | Basic and acidic residues | ||||
Sequence: RSPGERLDAQRPRPPQKPHHE | ||||||
Alternative sequence | VSP_020622 | 569-572 | in isoform b | |||
Sequence: EPRV → GDLN | ||||||
Alternative sequence | VSP_020623 | 573-813 | in isoform b | |||
Sequence: Missing | ||||||
Sequence conflict | 801 | in Ref. 1; AAA28142 | ||||
Sequence: A → R |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L15347 EMBL· GenBank· DDBJ | AAA28142.1 EMBL· GenBank· DDBJ | mRNA | ||
AY455928 EMBL· GenBank· DDBJ | AAR26307.1 EMBL· GenBank· DDBJ | mRNA | ||
AY493413 EMBL· GenBank· DDBJ | AAR86712.1 EMBL· GenBank· DDBJ | mRNA | ||
AY493414 EMBL· GenBank· DDBJ | AAR86713.1 EMBL· GenBank· DDBJ | mRNA | ||
FO080249 EMBL· GenBank· DDBJ | CCD62343.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FO080249 EMBL· GenBank· DDBJ | CCD62344.1 EMBL· GenBank· DDBJ | Genomic DNA |