Q07100 · PP2A3_ARATH
- ProteinSerine/threonine-protein phosphatase PP2A-3 catalytic subunit
- GenePP2A3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids313 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Functions redundantly with PP2A4, and is involved in establishing auxin gradients, apical-basal axis of polarity and root and shoot apical meristem during embryogenesis. May dephosphorylate PIN1 and regulate its subcellular distribution for polar auxin transport (PubMed:23167545).
Involved in the regulation of formative cell division in roots by dephosphorylating ACR4 protein kinase (PubMed:26792519).
Involved in the regulation of formative cell division in roots by dephosphorylating ACR4 protein kinase (PubMed:26792519).
Catalytic activity
- H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] + phosphate
Cofactor
Note: Binds 2 manganese ions per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 61 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 63 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 89 | Mn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 89 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 121 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Active site | 122 | Proton donor | ||||
Sequence: H | ||||||
Binding site | 171 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 245 | Mn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Molecular Function | metal ion binding | |
Molecular Function | myosin phosphatase activity | |
Molecular Function | phosphoprotein phosphatase activity | |
Biological Process | primary root development | |
Biological Process | root development | |
Biological Process | stem cell differentiation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSerine/threonine-protein phosphatase PP2A-3 catalytic subunit
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ07100
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000058854 | 1-313 | Serine/threonine-protein phosphatase PP2A-3 catalytic subunit | |||
Sequence: MGANSIPTDATIDLDEQISQLMQCKPLSEQQVRALCEKAKEILMDESNVQPVKSPVTICGDIHGQFHDLAELFRIGGMCPDTNYLFMGDYVDRGYYSVETVTLLVALKMRYPQRITILRGNHESRQITQVYGFYDECLRKYGNANVWKIFTDLFDYFPLTALVESEIFCLHGGLSPSIETLDNIRNFDRVQEVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDISEQFNHTNNLKLIARAHQLVMDGYNWAHEQKVVTIFSAPNYCYRCGNMASILEVDDCRNHTFIQFEPAPRRGEPDVTRRTPDYFL | ||||||
Modified residue | 313 | Leucine methyl ester | ||||
Sequence: L |
Post-translational modification
Reversibly methyl esterified on Leu-313 by leucine carboxyl methyltransferase 1 (LCMT1) and pectin methylesterase 1 (PME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
Keywords
- PTM
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) (By similarity).
Interacts with ACR4 (PubMed:26792519).
Interacts with TAP46 (PubMed:21216945, PubMed:24357600).
Interacts with SIC/RON3 (PubMed:26888284).
Interacts with ACR4 (PubMed:26792519).
Interacts with TAP46 (PubMed:21216945, PubMed:24357600).
Interacts with SIC/RON3 (PubMed:26888284).
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
Q07100-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length313
- Mass (Da)35,833
- Last updated1996-02-01 v2
- Checksum2EF8542AD8A89C64
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8AYS4 | A0A1P8AYS4_ARATH | PP2A-3 | 180 | ||
A0A1P8AYV1 | A0A1P8AYV1_ARATH | PP2A-3 | 178 | ||
F4IN36 | F4IN36_ARATH | PP2A-3 | 266 | ||
F4IN38 | F4IN38_ARATH | PP2A-3 | 171 |
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 15 | in Ref. 2; AAD10854 | ||||
Sequence: D → N |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M96841 EMBL· GenBank· DDBJ | AAA64742.1 EMBL· GenBank· DDBJ | mRNA | ||
U60135 EMBL· GenBank· DDBJ | AAD10854.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AC005956 EMBL· GenBank· DDBJ | AAD23731.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AC007087 EMBL· GenBank· DDBJ | AAM15383.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC10127.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT010166 EMBL· GenBank· DDBJ | AAQ22635.1 EMBL· GenBank· DDBJ | mRNA |