Q07100 · PP2A3_ARATH

Function

function

Functions redundantly with PP2A4, and is involved in establishing auxin gradients, apical-basal axis of polarity and root and shoot apical meristem during embryogenesis. May dephosphorylate PIN1 and regulate its subcellular distribution for polar auxin transport (PubMed:23167545).
Involved in the regulation of formative cell division in roots by dephosphorylating ACR4 protein kinase (PubMed:26792519).

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 manganese ions per subunit.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site61Mn2+ 1 (UniProtKB | ChEBI)
Binding site63Mn2+ 1 (UniProtKB | ChEBI)
Binding site89Mn2+ 1 (UniProtKB | ChEBI)
Binding site89Mn2+ 2 (UniProtKB | ChEBI)
Binding site121Mn2+ 2 (UniProtKB | ChEBI)
Active site122Proton donor
Binding site171Mn2+ 2 (UniProtKB | ChEBI)
Binding site245Mn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentnucleus
Molecular Functionmetal ion binding
Molecular Functionmyosin phosphatase activity
Molecular Functionphosphoprotein phosphatase activity
Biological Processprimary root development
Biological Processroot development
Biological Processstem cell differentiation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Serine/threonine-protein phosphatase PP2A-3 catalytic subunit
  • EC number
  • Alternative names
    • Protein phosphatase 2A isoform 3

Gene names

    • Name
      PP2A3
    • Synonyms
      PP2A4
    • ORF names
      MHK10.22
    • Ordered locus names
      At2g42500

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Columbia GL1
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q07100
  • Secondary accessions
    • Q7G9R3
    • Q9ZRF6

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000588541-313Serine/threonine-protein phosphatase PP2A-3 catalytic subunit
Modified residue313Leucine methyl ester

Post-translational modification

Reversibly methyl esterified on Leu-313 by leucine carboxyl methyltransferase 1 (LCMT1) and pectin methylesterase 1 (PME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.

Keywords

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) (By similarity).
Interacts with ACR4 (PubMed:26792519).
Interacts with TAP46 (PubMed:21216945, PubMed:24357600).
Interacts with SIC/RON3 (PubMed:26888284).

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the PPP phosphatase family. PP-2A subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete

This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.

Q07100-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    313
  • Mass (Da)
    35,833
  • Last updated
    1996-02-01 v2
  • Checksum
    2EF8542AD8A89C64
MGANSIPTDATIDLDEQISQLMQCKPLSEQQVRALCEKAKEILMDESNVQPVKSPVTICGDIHGQFHDLAELFRIGGMCPDTNYLFMGDYVDRGYYSVETVTLLVALKMRYPQRITILRGNHESRQITQVYGFYDECLRKYGNANVWKIFTDLFDYFPLTALVESEIFCLHGGLSPSIETLDNIRNFDRVQEVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDISEQFNHTNNLKLIARAHQLVMDGYNWAHEQKVVTIFSAPNYCYRCGNMASILEVDDCRNHTFIQFEPAPRRGEPDVTRRTPDYFL

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1P8AYS4A0A1P8AYS4_ARATHPP2A-3180
A0A1P8AYV1A0A1P8AYV1_ARATHPP2A-3178
F4IN36F4IN36_ARATHPP2A-3266
F4IN38F4IN38_ARATHPP2A-3171

Sequence caution

The sequence AAD10854.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict15in Ref. 2; AAD10854

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M96841
EMBL· GenBank· DDBJ
AAA64742.1
EMBL· GenBank· DDBJ
mRNA
U60135
EMBL· GenBank· DDBJ
AAD10854.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AC005956
EMBL· GenBank· DDBJ
AAD23731.1
EMBL· GenBank· DDBJ
Genomic DNA
AC007087
EMBL· GenBank· DDBJ
AAM15383.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002685
EMBL· GenBank· DDBJ
AEC10127.1
EMBL· GenBank· DDBJ
Genomic DNA
BT010166
EMBL· GenBank· DDBJ
AAQ22635.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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