Q06833 · NVJ2_YEAST

Function

function

During endoplasmic reticulum (ER) stress or when cellular ceramide levels increase, induces contacts between the ER and medial-Golgi complex to facilitate non-vesicular transport of ceramides from the ER to the Golgi complex where they are converted to complex sphingolipids, preventing toxic ceramide accumulation.

Miscellaneous

Present with 36500 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcell periphery
Cellular Componentendoplasmic reticulum
Cellular Componentendoplasmic reticulum membrane
Cellular Componentendoplasmic reticulum-Golgi intermediate compartment membrane
Cellular ComponentERMES complex
Cellular Componentnuclear membrane
Cellular Componentnucleus-vacuole junction
Molecular Functionlipid binding
Molecular Functionlipid transporter activity
Biological ProcessER to Golgi ceramide transport
Biological Processmitochondrion-endoplasmic reticulum membrane tethering
Biological Processphospholipid transport

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nucleus-vacuole junction protein 2

Gene names

    • Name
      NVJ2
    • Ordered locus names
      YPR091C

Organism names

Accessions

  • Primary accession
    Q06833
  • Secondary accessions
    • D6W491

Proteomes

Organism-specific databases

Subcellular Location

Endoplasmic reticulum membrane
; Single-pass type II membrane protein
Nucleus membrane
; Single-pass type II membrane protein
Note: Enriched at the nucleus-vacuole junction where it becomes increasingly concentrated as cells enter into the late-logarithmic growth phase (PubMed:22250200).
During endoplasmic reticulum (ER) stress, localizes to ER-Golgi contacts (PubMed:28011845).

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-5Cytoplasmic
Transmembrane6-26Helical; Signal-anchor for type II membrane protein
Topological domain27-770Lumenal

Keywords

Phenotypes & Variants

Disruption phenotype

Leads to abnormal cellular complex sphingolipid levels (PubMed:36897280).
Simultaneous knockout of SVF1 to leads to myriocin sensitivity (sphingosine biosynthesis inhibitor) (PubMed:36897280).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis340Failure to mediate ceramide transport; when associated with A-472.
Mutagenesis472Failure to mediate ceramide transport; when associated with A-340.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 15 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, glycosylation, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002578151-770Nucleus-vacuole junction protein 2
Glycosylation228N-linked (GlcNAc...) asparagine
Glycosylation263N-linked (GlcNAc...) asparagine
Glycosylation279N-linked (GlcNAc...) asparagine
Glycosylation300N-linked (GlcNAc...) asparagine
Glycosylation391N-linked (GlcNAc...) asparagine
Glycosylation528N-linked (GlcNAc...) asparagine
Glycosylation529N-linked (GlcNAc...) asparagine
Glycosylation595N-linked (GlcNAc...) asparagine
Glycosylation620N-linked (GlcNAc...) asparagine
Modified residue640Phosphoserine
Modified residue669Phosphoserine
Glycosylation700N-linked (GlcNAc...) asparagine
Modified residue717Phosphoserine
Glycosylation718N-linked (GlcNAc...) asparagine
Modified residue720Phosphoserine
Modified residue723Phosphoserine

Keywords

Proteomic databases

PTM databases

Interaction

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q06833BBC1 P470682EBI-37290, EBI-3437
BINARY Q06833RVS167 P397433EBI-37290, EBI-14500

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain114-266PH
Domain304-504SMP-LTD
Region541-566Disordered
Compositional bias548-566Basic and acidic residues
Region578-600Disordered
Region615-770Disordered
Compositional bias629-646Basic and acidic residues
Compositional bias691-728Polar residues
Compositional bias739-754Polar residues
Compositional bias756-770Basic and acidic residues

Domain

The SMP-LTD domain is a barrel-like domain that can bind various types of glycerophospholipids in its interior and mediate their transfer between two adjacent bilayers.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    770
  • Mass (Da)
    87,324
  • Last updated
    1996-11-01 v1
  • Checksum
    E4A1EF69AA49E06A
MASLKVFLAVYLLGGITFLPLVLFTLYKIHLLYSNLKSASKKELDHDTADEIDEKTRLLARDIDPEFKARKLEEQLGVKVFNKGWITVTKQYYYHSSEVAVILKNSNNNKDSDTALQEQILQRTDLKKKQRFFAVLRHGNLFLYKDDSQNANLVHAISLQNRFITIWPRFDELGKEELPDASLFTKRTCIAIFKNDLVSIDSKNHNVILPHFDPLTSAESNNGDISTNDTTHEYQSQFHSSNQFFLYFDNNMDKEDWYYQLINASKNSNSLSTGLLDPNVSANAAHLKTKDMLQLIQDINSTENQLTTKWLNALLGRLFLSLQQTDTLNKFIHEKICKKLNKIKTPGFLDDLVVEKVDVGDSAPLFTSPELLELSPEGSTKIAIDVQYRGNLTIIIATKASINLGSRFKQREVSLQLSIKIKEFSGPLLFLIKPPPSNRIWYAFRTEPIMDFEIEPIVSSSKLSYNVVTNAIKSKFAEAVKESLVVPFMDDIVFYPTPNEVYRGGIWEEQDPEAAARARTAAAASDMNNTSAKEHLEALQEGGMKTQSRIKKALRPERKKENLKDLVDASGVATKTTTQTTVTTATNDDVSSSENSTKSRKYFKNSIKKIGRWYKDNVGNSSDTEDMDEIDVQDKKNDDSADERESDNPILTSNPKMISNRRPVPRRPSQPLNTLSPKLEGRKEKDTENFPVPPSASNMNASKMFANKENRKFSVSSNDSQNSLKNGDPHVKASKLESSQAFVKKTSQNRFNDGFFKQDLEFEEQREPKL

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias548-566Basic and acidic residues
Compositional bias629-646Basic and acidic residues
Compositional bias691-728Polar residues
Compositional bias739-754Polar residues
Compositional bias756-770Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U51033
EMBL· GenBank· DDBJ
AAB68138.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006949
EMBL· GenBank· DDBJ
DAA11507.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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