Q06833 · NVJ2_YEAST
- ProteinNucleus-vacuole junction protein 2
- GeneNVJ2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids770 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
During endoplasmic reticulum (ER) stress or when cellular ceramide levels increase, induces contacts between the ER and medial-Golgi complex to facilitate non-vesicular transport of ceramides from the ER to the Golgi complex where they are converted to complex sphingolipids, preventing toxic ceramide accumulation.
Miscellaneous
Present with 36500 molecules/cell in log phase SD medium.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cell periphery | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | endoplasmic reticulum-Golgi intermediate compartment membrane | |
Cellular Component | ERMES complex | |
Cellular Component | nuclear membrane | |
Cellular Component | nucleus-vacuole junction | |
Molecular Function | lipid binding | |
Molecular Function | lipid transporter activity | |
Biological Process | ER to Golgi ceramide transport | |
Biological Process | mitochondrion-endoplasmic reticulum membrane tethering | |
Biological Process | phospholipid transport |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNucleus-vacuole junction protein 2
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ06833
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass type II membrane protein
Nucleus membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-5 | Cytoplasmic | ||||
Sequence: MASLK | ||||||
Transmembrane | 6-26 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: VFLAVYLLGGITFLPLVLFTL | ||||||
Topological domain | 27-770 | Lumenal | ||||
Sequence: YKIHLLYSNLKSASKKELDHDTADEIDEKTRLLARDIDPEFKARKLEEQLGVKVFNKGWITVTKQYYYHSSEVAVILKNSNNNKDSDTALQEQILQRTDLKKKQRFFAVLRHGNLFLYKDDSQNANLVHAISLQNRFITIWPRFDELGKEELPDASLFTKRTCIAIFKNDLVSIDSKNHNVILPHFDPLTSAESNNGDISTNDTTHEYQSQFHSSNQFFLYFDNNMDKEDWYYQLINASKNSNSLSTGLLDPNVSANAAHLKTKDMLQLIQDINSTENQLTTKWLNALLGRLFLSLQQTDTLNKFIHEKICKKLNKIKTPGFLDDLVVEKVDVGDSAPLFTSPELLELSPEGSTKIAIDVQYRGNLTIIIATKASINLGSRFKQREVSLQLSIKIKEFSGPLLFLIKPPPSNRIWYAFRTEPIMDFEIEPIVSSSKLSYNVVTNAIKSKFAEAVKESLVVPFMDDIVFYPTPNEVYRGGIWEEQDPEAAARARTAAAASDMNNTSAKEHLEALQEGGMKTQSRIKKALRPERKKENLKDLVDASGVATKTTTQTTVTTATNDDVSSSENSTKSRKYFKNSIKKIGRWYKDNVGNSSDTEDMDEIDVQDKKNDDSADERESDNPILTSNPKMISNRRPVPRRPSQPLNTLSPKLEGRKEKDTENFPVPPSASNMNASKMFANKENRKFSVSSNDSQNSLKNGDPHVKASKLESSQAFVKKTSQNRFNDGFFKQDLEFEEQREPKL |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Leads to abnormal cellular complex sphingolipid levels (PubMed:36897280).
Simultaneous knockout of SVF1 to leads to myriocin sensitivity (sphingosine biosynthesis inhibitor) (PubMed:36897280).
Simultaneous knockout of SVF1 to leads to myriocin sensitivity (sphingosine biosynthesis inhibitor) (PubMed:36897280).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 340 | Failure to mediate ceramide transport; when associated with A-472. | ||||
Sequence: L → A | ||||||
Mutagenesis | 472 | Failure to mediate ceramide transport; when associated with A-340. | ||||
Sequence: I → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 15 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, glycosylation, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000257815 | 1-770 | Nucleus-vacuole junction protein 2 | |||
Sequence: MASLKVFLAVYLLGGITFLPLVLFTLYKIHLLYSNLKSASKKELDHDTADEIDEKTRLLARDIDPEFKARKLEEQLGVKVFNKGWITVTKQYYYHSSEVAVILKNSNNNKDSDTALQEQILQRTDLKKKQRFFAVLRHGNLFLYKDDSQNANLVHAISLQNRFITIWPRFDELGKEELPDASLFTKRTCIAIFKNDLVSIDSKNHNVILPHFDPLTSAESNNGDISTNDTTHEYQSQFHSSNQFFLYFDNNMDKEDWYYQLINASKNSNSLSTGLLDPNVSANAAHLKTKDMLQLIQDINSTENQLTTKWLNALLGRLFLSLQQTDTLNKFIHEKICKKLNKIKTPGFLDDLVVEKVDVGDSAPLFTSPELLELSPEGSTKIAIDVQYRGNLTIIIATKASINLGSRFKQREVSLQLSIKIKEFSGPLLFLIKPPPSNRIWYAFRTEPIMDFEIEPIVSSSKLSYNVVTNAIKSKFAEAVKESLVVPFMDDIVFYPTPNEVYRGGIWEEQDPEAAARARTAAAASDMNNTSAKEHLEALQEGGMKTQSRIKKALRPERKKENLKDLVDASGVATKTTTQTTVTTATNDDVSSSENSTKSRKYFKNSIKKIGRWYKDNVGNSSDTEDMDEIDVQDKKNDDSADERESDNPILTSNPKMISNRRPVPRRPSQPLNTLSPKLEGRKEKDTENFPVPPSASNMNASKMFANKENRKFSVSSNDSQNSLKNGDPHVKASKLESSQAFVKKTSQNRFNDGFFKQDLEFEEQREPKL | ||||||
Glycosylation | 228 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 263 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 279 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 300 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 391 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 528 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 529 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 595 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 620 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Modified residue | 640 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 669 | Phosphoserine | ||||
Sequence: S | ||||||
Glycosylation | 700 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Modified residue | 717 | Phosphoserine | ||||
Sequence: S | ||||||
Glycosylation | 718 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Modified residue | 720 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 723 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q06833 | BBC1 P47068 | 2 | EBI-37290, EBI-3437 | |
BINARY | Q06833 | RVS167 P39743 | 3 | EBI-37290, EBI-14500 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 114-266 | PH | ||||
Sequence: TALQEQILQRTDLKKKQRFFAVLRHGNLFLYKDDSQNANLVHAISLQNRFITIWPRFDELGKEELPDASLFTKRTCIAIFKNDLVSIDSKNHNVILPHFDPLTSAESNNGDISTNDTTHEYQSQFHSSNQFFLYFDNNMDKEDWYYQLINASK | ||||||
Domain | 304-504 | SMP-LTD | ||||
Sequence: NQLTTKWLNALLGRLFLSLQQTDTLNKFIHEKICKKLNKIKTPGFLDDLVVEKVDVGDSAPLFTSPELLELSPEGSTKIAIDVQYRGNLTIIIATKASINLGSRFKQREVSLQLSIKIKEFSGPLLFLIKPPPSNRIWYAFRTEPIMDFEIEPIVSSSKLSYNVVTNAIKSKFAEAVKESLVVPFMDDIVFYPTPNEVYRG | ||||||
Region | 541-566 | Disordered | ||||
Sequence: EGGMKTQSRIKKALRPERKKENLKDL | ||||||
Compositional bias | 548-566 | Basic and acidic residues | ||||
Sequence: SRIKKALRPERKKENLKDL | ||||||
Region | 578-600 | Disordered | ||||
Sequence: TQTTVTTATNDDVSSSENSTKSR | ||||||
Region | 615-770 | Disordered | ||||
Sequence: KDNVGNSSDTEDMDEIDVQDKKNDDSADERESDNPILTSNPKMISNRRPVPRRPSQPLNTLSPKLEGRKEKDTENFPVPPSASNMNASKMFANKENRKFSVSSNDSQNSLKNGDPHVKASKLESSQAFVKKTSQNRFNDGFFKQDLEFEEQREPKL | ||||||
Compositional bias | 629-646 | Basic and acidic residues | ||||
Sequence: EIDVQDKKNDDSADERES | ||||||
Compositional bias | 691-728 | Polar residues | ||||
Sequence: PVPPSASNMNASKMFANKENRKFSVSSNDSQNSLKNGD | ||||||
Compositional bias | 739-754 | Polar residues | ||||
Sequence: SQAFVKKTSQNRFNDG | ||||||
Compositional bias | 756-770 | Basic and acidic residues | ||||
Sequence: FKQDLEFEEQREPKL |
Domain
The SMP-LTD domain is a barrel-like domain that can bind various types of glycerophospholipids in its interior and mediate their transfer between two adjacent bilayers.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length770
- Mass (Da)87,324
- Last updated1996-11-01 v1
- ChecksumE4A1EF69AA49E06A
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 548-566 | Basic and acidic residues | ||||
Sequence: SRIKKALRPERKKENLKDL | ||||||
Compositional bias | 629-646 | Basic and acidic residues | ||||
Sequence: EIDVQDKKNDDSADERES | ||||||
Compositional bias | 691-728 | Polar residues | ||||
Sequence: PVPPSASNMNASKMFANKENRKFSVSSNDSQNSLKNGD | ||||||
Compositional bias | 739-754 | Polar residues | ||||
Sequence: SQAFVKKTSQNRFNDG | ||||||
Compositional bias | 756-770 | Basic and acidic residues | ||||
Sequence: FKQDLEFEEQREPKL |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U51033 EMBL· GenBank· DDBJ | AAB68138.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006949 EMBL· GenBank· DDBJ | DAA11507.1 EMBL· GenBank· DDBJ | Genomic DNA |