Q06696 · VPS36_YEAST

Function

function

Component of the ESCRT-II complex, which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs (PubMed:12194858, PubMed:15329733, PubMed:15469844).
The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation (PubMed:12194858).
The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex (PubMed:12194858).
Involved in the trafficking of the plasma membrane ATPase (PubMed:12194858).
Its ability to bind ubiquitin plays a central role in endosomal sorting of ubiquitinated cargo proteins by the ESCRT complexes (PubMed:15029239).

Miscellaneous

Present with 2470 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular ComponentESCRT II complex
Cellular Componentlate endosome membrane
Molecular Functionmetal ion binding
Molecular Functionphosphatidylinositol-3-phosphate binding
Molecular Functionubiquitin binding
Biological ProcessATP export
Biological Processcarbon catabolite repression of transcription from RNA polymerase II promoter by glucose
Biological Processcytoplasm to vacuole targeting by the Cvt pathway
Biological Processmacroautophagy
Biological Processprotein retention in Golgi apparatus
Biological Processprotein targeting to vacuole
Biological Processprotein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
Biological Processubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

Keywords

Enzyme and pathway databases

Protein family/group databases

    • 3.A.31.1.1the endosomal sorting complexes required for transport iii (escrt-iii) family

Names & Taxonomy

Protein names

  • Recommended name
    Vacuolar protein-sorting-associated protein 36
  • Alternative names
    • ESCRT-II complex subunit VPS36

Gene names

    • Name
      VPS36
    • Synonyms
      GRD12, VAC3, VPL11
    • Ordered locus names
      YLR417W

Organism names

Accessions

  • Primary accession
    Q06696
  • Secondary accessions
    • D6VZ53

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Endosome membrane
; Peripheral membrane protein

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis187-188Abolishes ubiquitin-binding and vacuole sorting of ubiquitinated proteins.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 9 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002152271-566Vacuolar protein-sorting-associated protein 36

Proteomic databases

PTM databases

Interaction

Subunit

Component of the endosomal sorting required for transport complex II (ESCRT-II), which consists of 2 copies of VPS25, 1 copy of SNF8, and 1 copy of VPS36 (PubMed:15329733, PubMed:15469844).
The ESCRT-II complex interacts directly with the VPS20 subunit of the ESCRT-III complex (PubMed:12194858).
Binds ubiquitin (PubMed:15029239).

Binary interactions

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain, zinc finger.

TypeIDPosition(s)Description
Domain7-96GLUE N-terminal
Zinc finger114-151RanBP2-type 1; degenerate
Zinc finger177-205RanBP2-type 2
Domain255-288GLUE C-terminal

Domain

The second RanBP2-type zinc-finger is functional and binds ubiquitin.

Sequence similarities

Belongs to the VPS36 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    566
  • Mass (Da)
    64,018
  • Last updated
    1996-11-01 v1
  • Checksum
    60DDD4EA3A620409
MEYWHYVETTSSGQPLLREGEKDIFIDQSVGLYHGKSKILQRQRGRIFLTSQRIIYIDDAKPTQNSLGLELDDLAYVNYSSGFLTRSPRLILFFKDPSSKDELGKSAETASADVVSTWVCPICMVSNETQGEFTKDTLPTPICINCGVPADYELTKSSINCSNAIDPNANPQNQFGVNSENICPACTFANHPQIGNCEICGHRLPNASKVRSKLNRLNFHDSRVHIELEKNSLARNKSSHSALSSSSSTGSSTEFVQLSFRKSDGVLFSQATERALENILTEKNKHIFNQNVVSVNGVDMRKGASSHEYNNEVPFIETKLSRIGISSLEKSRENQLLNNDILFNNALTDLNKLMSLATSIERLYKNSNITMKTKTLNLQDESTVNEPKTRRPLLILDREKFLNKELFLDEIAREIYEFTLSEFKDLNSDTNYMIITLVDLYAMYNKSMRIGTGLISPMEMREACERFEHLGLNELKLVKVNKRILCVTSEKFDVVKEKLVDLIGDNPGSDLLRLTQILSSNNSKSNWTLGILMEVLQNCVDEGDLLIDKQLSGIYYYKNSYWPSHI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U20162
EMBL· GenBank· DDBJ
AAB67493.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006945
EMBL· GenBank· DDBJ
DAA09719.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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