Q06486 · KC1D_RAT
- ProteinCasein kinase I isoform delta
- GeneCsnk1d
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids415 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Essential serine/threonine-protein kinase that regulates diverse cellular growth and survival processes including Wnt signaling, DNA repair and circadian rhythms. It can phosphorylate a large number of proteins. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Phosphorylates connexin-43/GJA1, MAP1A, SNAPIN, MAPT/TAU, TOP2A, DCK, HIF1A, EIF6, p53/TP53, DVL2, DVL3, ESR1, AIB1/NCOA3, DNMT1, PKD2, YAP1, PER1 and PER2. Central component of the circadian clock. In balance with PP1, determines the circadian period length through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. YAP1 phosphorylation promotes its SCF(beta-TRCP) E3 ubiquitin ligase-mediated ubiquitination and subsequent degradation. DNMT1 phosphorylation reduces its DNA-binding activity. Phosphorylation of ESR1 and AIB1/NCOA3 stimulates their activity and coactivation. Phosphorylation of DVL2 and DVL3 regulates WNT3A signaling pathway that controls neurite outgrowth. Phosphorylates NEDD9/HEF1 (By similarity).
EIF6 phosphorylation promotes its nuclear export. Triggers down-regulation of dopamine receptors in the forebrain. Activates DCK in vitro by phosphorylation. TOP2A phosphorylation favors DNA cleavable complex formation. May regulate the formation of the mitotic spindle apparatus in extravillous trophoblast. Modulates connexin-43/GJA1 gap junction assembly by phosphorylation. Probably involved in lymphocyte physiology. Regulates fast synaptic transmission mediated by glutamate (By similarity).
EIF6 phosphorylation promotes its nuclear export. Triggers down-regulation of dopamine receptors in the forebrain. Activates DCK in vitro by phosphorylation. TOP2A phosphorylation favors DNA cleavable complex formation. May regulate the formation of the mitotic spindle apparatus in extravillous trophoblast. Modulates connexin-43/GJA1 gap junction assembly by phosphorylation. Probably involved in lymphocyte physiology. Regulates fast synaptic transmission mediated by glutamate (By similarity).
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]This reaction proceeds in the forward direction.
Activity regulation
Drug-mediated inhibition leads to a delay of the oscillations with the magnitude of this effect dependent upon the timing of drug administration. Inhibited by phosphorylation (By similarity).
Exhibits substrate-dependent heparin activation
Exhibits substrate-dependent heparin activation
Features
Showing features for binding site, active site.
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCasein kinase I isoform delta
- EC number
- Short namesCKI-delta
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ06486
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localized at mitotic spindle microtubules, and at the centrosomes and interphase in interphase cells. Recruited to the spindle apparatus and the centrosomes in response to DNA-damage. Correct subcellular localization requires kinase activity (By similarity).
Keywords
- Cellular component
Phenotypes & Variants
Chemistry
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000192836 | 1-415 | Casein kinase I isoform delta | |||
Sequence: MELRVGNRYRLGRKIGSGSFGDIYLGTDIAAGEEVAIKLECVKTKHPQLHIESKIYKMMQGGVGIPTIRWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGASRAADDAERERRDREERLRHSRNPATRGLPSTASGRLRGTQEVAPPTPLTPTSHTANTSPRPVSGMERERKVSMRLHRGAPVNVSSSDLTGRQDTSRMSTSQIPGRVASSGLQSVVHR | ||||||
Modified residue | 328 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 331 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 370 | Phosphoserine; by PKA | ||||
Sequence: S | ||||||
Modified residue | 375 | Omega-N-methylarginine | ||||
Sequence: R | ||||||
Modified residue | 382 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 383 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 384 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 407 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 411 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Autophosphorylated on serine and threonine residues; this autophosphorylation represses activity. Reactivated by phosphatase-mediated dephosphorylation. May be dephosphorylated by PP1.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in most tissues, including heart, brain, testis, kidney, spleen and lung.
Gene expression databases
Interaction
Subunit
Monomer (By similarity).
Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, ARTNL/BMAL1 or ARTNL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins (By similarity).
Interacts with DNMT1 (By similarity).
Interacts directly with PER1 and PER2 which may lead to their degradation (By similarity).
Interacts with MAPT/TAU, SNAPIN, DBNDD2, AIB1/NCOA3 and ESR1 (By similarity).
Interacts with AKAP9/AKAP450; this interaction promotes centrosomal subcellular location (By similarity).
Binds to tubulins in mitotic cells upon DNA damage (By similarity).
Interacts with GJA1 (By similarity).
Interacts with MAP1A (PubMed:15961172).
Interacts with DDX3X; this interaction enhances CSNK1D kinase activity in vitro, but it is unclear whether this interaction is physiologically relevant (By similarity).
Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, ARTNL/BMAL1 or ARTNL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins (By similarity).
Interacts with DNMT1 (By similarity).
Interacts directly with PER1 and PER2 which may lead to their degradation (By similarity).
Interacts with MAPT/TAU, SNAPIN, DBNDD2, AIB1/NCOA3 and ESR1 (By similarity).
Interacts with AKAP9/AKAP450; this interaction promotes centrosomal subcellular location (By similarity).
Binds to tubulins in mitotic cells upon DNA damage (By similarity).
Interacts with GJA1 (By similarity).
Interacts with MAP1A (PubMed:15961172).
Interacts with DDX3X; this interaction enhances CSNK1D kinase activity in vitro, but it is unclear whether this interaction is physiologically relevant (By similarity).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
XENO | Q06486 | MDM2 Q00987 | 2 | EBI-2910316, EBI-389668 | |
XENO | Q06486-2 | Snapin Q9Z266 | 4 | EBI-7088890, EBI-6170320 |
Protein-protein interaction databases
Chemistry
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 9-277 | Protein kinase | ||||
Sequence: YRLGRKIGSGSFGDIYLGTDIAAGEEVAIKLECVKTKHPQLHIESKIYKMMQGGVGIPTIRWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYLRQLFRNLF | ||||||
Region | 278-364 | Centrosomal localization signal (CLS) | ||||
Sequence: HRQGFSYDYVFDWNMLKFGASRAADDAERERRDREERLRHSRNPATRGLPSTASGRLRGTQEVAPPTPLTPTSHTANTSPRPVSGME | ||||||
Compositional bias | 301-320 | Basic and acidic residues | ||||
Sequence: ADDAERERRDREERLRHSRN | ||||||
Region | 301-415 | Disordered | ||||
Sequence: ADDAERERRDREERLRHSRNPATRGLPSTASGRLRGTQEVAPPTPLTPTSHTANTSPRPVSGMERERKVSMRLHRGAPVNVSSSDLTGRQDTSRMSTSQIPGRVASSGLQSVVHR | ||||||
Region | 317-342 | Autoinhibitory | ||||
Sequence: HSRNPATRGLPSTASGRLRGTQEVAP | ||||||
Compositional bias | 342-359 | Polar residues | ||||
Sequence: PPTPLTPTSHTANTSPRP | ||||||
Compositional bias | 380-415 | Polar residues | ||||
Sequence: NVSSSDLTGRQDTSRMSTSQIPGRVASSGLQSVVHR |
Domain
The autoinhibitory domain is involved in regulating enzyme activity through autophosphorylation and possibly, through heparin binding.
Sequence similarities
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q06486-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length415
- Mass (Da)47,316
- Last updated2004-05-10 v2
- ChecksumB97F04AF9EB466D2
Q06486-2
- Name2
- Differences from canonical
- 400-415: IPGRVASSGLQSVVHR → RSRDMASLRLHAARQGARCRPQRPRRTTY
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I6AHE0 | A0A8I6AHE0_RAT | Csnk1d | 402 | ||
A0A8L2UMH1 | A0A8L2UMH1_RAT | Csnk1d | 409 | ||
A0A8I5ZSZ2 | A0A8I5ZSZ2_RAT | Csnk1d | 410 |
Features
Showing features for compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 301-320 | Basic and acidic residues | ||||
Sequence: ADDAERERRDREERLRHSRN | ||||||
Compositional bias | 342-359 | Polar residues | ||||
Sequence: PPTPLTPTSHTANTSPRP | ||||||
Compositional bias | 380-415 | Polar residues | ||||
Sequence: NVSSSDLTGRQDTSRMSTSQIPGRVASSGLQSVVHR | ||||||
Alternative sequence | VSP_010255 | 400-415 | in isoform 2 | |||
Sequence: IPGRVASSGLQSVVHR → RSRDMASLRLHAARQGARCRPQRPRRTTY |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L07578 EMBL· GenBank· DDBJ | AAA40934.1 EMBL· GenBank· DDBJ | mRNA | ||
AB063114 EMBL· GenBank· DDBJ | BAB60852.1 EMBL· GenBank· DDBJ | mRNA |