Q06389 · NCS1_YEAST
- ProteinCalcium-binding protein NCS-1
- GeneFRQ1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids190 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Regulator of phosphatidylinositol 4-kinase PIK1.
Miscellaneous
Binds 3 calcium ions via the second, third and fourth EF-hand.
Present with 7160 molecules/cell in log phase SD medium.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 73 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 75 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 77 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 79 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: F | ||||||
Binding site | 84 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 109 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 111 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 113 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 115 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 120 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 157 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 159 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 161 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 163 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 168 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cellular bud membrane | |
Cellular Component | cytosol | |
Cellular Component | Golgi membrane | |
Cellular Component | intracellular membrane-bounded organelle | |
Cellular Component | membrane | |
Molecular Function | calcium ion binding | |
Molecular Function | enzyme activator activity | |
Biological Process | regulation of signal transduction |
Keywords
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCalcium-binding protein NCS-1
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ06389
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Bud membrane ; Peripheral membrane protein
Note: Buds and bud scars.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 2 | Abolishes N-myristoylation. | ||||
Sequence: G → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for initiator methionine, lipidation, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Lipidation | 2 | N-myristoyl glycine | ||||
Sequence: G | ||||||
Chain | PRO_0000073795 | 2-190 | Calcium-binding protein NCS-1 | |||
Sequence: GAKTSKLSKDDLTCLKQSTYFDRREIQQWHKGFLRDCPSGQLAREDFVKIYKQFFPFGSPEDFANHLFTVFDKDNNGFIHFEEFITVLSTTSRGTLEEKLSWAFELYDLNHDGYITFDEMLTIVASVYKMMGSMVTLNEDEATPEMRVKKIFKLMDKNEDGYITLDEFREGSKVDPSIIGALNLYDGLI |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Binds PIK1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q06389 | PEX14 P53112 | 3 | EBI-11946, EBI-13212 | |
BINARY | Q06389 | PIK1 P39104 | 4 | EBI-11946, EBI-13423 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 24-59 | EF-hand 1 | ||||
Sequence: RREIQQWHKGFLRDCPSGQLAREDFVKIYKQFFPFG | ||||||
Domain | 60-95 | EF-hand 2 | ||||
Sequence: SPEDFANHLFTVFDKDNNGFIHFEEFITVLSTTSRG | ||||||
Domain | 96-131 | EF-hand 3 | ||||
Sequence: TLEEKLSWAFELYDLNHDGYITFDEMLTIVASVYKM | ||||||
Domain | 144-179 | EF-hand 4 | ||||
Sequence: TPEMRVKKIFKLMDKNEDGYITLDEFREGSKVDPSI |
Sequence similarities
Belongs to the recoverin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length190
- Mass (Da)22,011
- Last updated2007-01-23 v2
- Checksum5E9D7F48F8AADA21
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U28373 EMBL· GenBank· DDBJ | AAB64809.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006938 EMBL· GenBank· DDBJ | DAA12214.1 EMBL· GenBank· DDBJ | Genomic DNA |