Q06320 · CWLC_BACSU

  • Protein
    Sporulation-specific N-acetylmuramoyl-L-alanine amidase
  • Gene
    cwlC
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. CwlC is able to hydrolyze type A cell walls such as B.subtilis. Its main function is to lyze the mother cell wall peptidoglycan, playing a role during sporulation.

Catalytic activity

  • Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
    EC:3.5.1.28 (UniProtKB | ENZYME | Rhea)

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Activity regulation

Inhibited by EDTA.

pH Dependence

Optimum pH is 8.5-9.5.

Temperature Dependence

Optimum temperature is 60 degrees Celsius.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site10Zn2+ (UniProtKB | ChEBI)
Binding site24Zn2+ (UniProtKB | ChEBI)
Binding site79Zn2+ (UniProtKB | ChEBI)
Active site141

GO annotations

AspectTerm
Cellular Componentextracellular region
Cellular Componentouter membrane-bounded periplasmic space
Molecular Functionmetal ion binding
Molecular FunctionN-acetylmuramoyl-L-alanine amidase activity
Molecular Functionpeptidoglycan binding
Biological Processcell wall organization
Biological Processestablishment of competence for transformation
Biological Processpeptidoglycan catabolic process
Biological Processsporulation resulting in formation of a cellular spore

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Sporulation-specific N-acetylmuramoyl-L-alanine amidase
  • EC number
  • Alternative names
    • Autolysin
    • Cell wall hydrolase

Gene names

    • Name
      cwlC
    • Ordered locus names
      BSU17410

Organism names

Accessions

  • Primary accession
    Q06320

Proteomes

Subcellular Location

Secreted, cell wall
Note: Is associated with the mother cell wall of sporulating cells.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis1023% of wild-type activity.
Mutagenesis24Loss of activity.
Mutagenesis55Loss of activity.
Mutagenesis79Loss of activity.
Mutagenesis7915% of wild-type activity.
Mutagenesis81Loss of activity.
Mutagenesis141Loss of activity.

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00001644182-255Sporulation-specific N-acetylmuramoyl-L-alanine amidase

Proteomic databases

Expression

Induction

Induced at 6 to 7 hours after sporulation.

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, repeat, region.

TypeIDPosition(s)Description
Domain4-172MurNAc-LAA
Domain180-254SPOR
Repeat184-2191
Region184-2552 X 35 AA approximate tandem repeats
Repeat220-2552

Domain

Contains an N-terminal catalytic domain and two C-terminal tandem repeat sequences that play an important role in peptidoglycan binding.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    255
  • Mass (Da)
    27,146
  • Last updated
    1995-02-01 v1
  • Checksum
    30C950116784FBC1
MVKIFIDPGHGGSDPGATGNGLQEKTLTLQIALALRTILTNEYEGVSLLLSRTSDQYVSLNDRTNAANNWGADFFLSIHVNSGGGTGFESYIYPDVGAPTTTYQSTIHSEVIQAVDFADRGKKTANFHVLRESAMPALLTENGFIDTVSDANKLKTSSFIQSLARGHANGLEQAFNLKKTSSSGLYKVQIGAFKVKANADSLASNAEAKGFDSIVLLKDGLYKVQIGAFSSKDNADTLAARAKNAGFDAIVILES

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict158in Ref. 5; CAA92813

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D14666
EMBL· GenBank· DDBJ
BAA03500.1
EMBL· GenBank· DDBJ
Genomic DNA
AL009126
EMBL· GenBank· DDBJ
CAB13625.2
EMBL· GenBank· DDBJ
Genomic DNA
Z68500
EMBL· GenBank· DDBJ
CAA92813.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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