Q06131 · SCTB_YERPS
- ProteinType 3 secretion system translocon protein SctB
- GenesctB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids306 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:22910185, PubMed:23297383).
YopB/SctE and YopD/SctB are inserted into the host membrane where they form a pore and allow the translocation of effector proteins into the cytosol of target cells (PubMed:23297383, PubMed:25684661, PubMed:8596454).
Essential for YopE, YopH and YopM translocation (PubMed:22910185, PubMed:25684661, PubMed:8596454).
YopB/SctE and YopD/SctB are inserted into the host membrane where they form a pore and allow the translocation of effector proteins into the cytosol of target cells (PubMed:23297383, PubMed:25684661, PubMed:8596454).
Essential for YopE, YopH and YopM translocation (PubMed:22910185, PubMed:25684661, PubMed:8596454).
Not required for IL-8 production and proinflammatory signaling response in host cells.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | host cell membrane | |
Cellular Component | membrane |
Keywords
- Biological process
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameType 3 secretion system translocon protein SctB
- Short namesT3SS translocon protein SctB
- Alternative names
Gene names
Encoded on
- Plasmid pIB1
- Plasmid pYV
Organism names
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Yersiniaceae > Yersinia
Accessions
- Primary accessionQ06131
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Host membrane ; Single-pass membrane protein
Note: Secreted via the type III secretion system (T3SS) (PubMed:21965570).
After export via the T3SS, YopB/SctE and YopD/SctB form a multimeric integral membrane complex in eukaryotic cell membranes (By similarity).
After export via the T3SS, YopB/SctE and YopD/SctB form a multimeric integral membrane complex in eukaryotic cell membranes (By similarity).
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 128-152 | Helical | ||||
Sequence: LMIAMAVVSGIMAATSTVASAFSIA |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
The yopBD mutant can secrete YopH at the same level as the wild-type strain in vivo but is unable to translocate YopH into the cytosol of HeLa cells (PubMed:8596454).
Much less YopE and, to some extent YopH, are detected in cytosolic fractions of HeLa cell monolayers infected with null mutant (PubMed:22910185).
A mutant lacking the transmembrane region forms smaller pores with delayed kinetics (PubMed:23297383).
Much less YopE and, to some extent YopH, are detected in cytosolic fractions of HeLa cell monolayers infected with null mutant (PubMed:22910185).
A mutant lacking the transmembrane region forms smaller pores with delayed kinetics (PubMed:23297383).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 5-19 | Lack of YopD/SctB secretion. Impairs the cytotoxicity response of eukaryotic cells. | ||||
Sequence: Missing | ||||||
Mutagenesis | 10-19 | Displays reduced YopD/SctB secretion levels. Maintains the capacity to efficiently intoxicate nonimmune epithelial cells with the YopE cytotoxin. | ||||
Sequence: Missing | ||||||
Mutagenesis | 11-19 | Retains high YopD/SctB secretion efficiency. Maintains the capacity to efficiently intoxicate nonimmune epithelial cells with the YopE cytotoxin. | ||||
Sequence: Missing | ||||||
Mutagenesis | 32 | Maintains full T3SS function in vitro, but mutant is attenuated for virulence in a murine oral-infection model. | ||||
Sequence: I → K or P | ||||||
Mutagenesis | 150-170 | Displays normal Yop secretion and macrophage pore formation, but displays significant defects in translocating Yop proteins. Mutant is defective in inhibiting ROS production by phagocytes and loses the ability to inhibit host cell death pathways. | ||||
Sequence: Missing | ||||||
Mutagenesis | 207-227 | Displays normal Yop secretion and macrophage pore formation, but displays significant defects in translocating Yop proteins. Mutant is defective in inhibiting ROS production by phagocytes and loses the ability to inhibit host cell death pathways. | ||||
Sequence: Missing |
Miscellaneous
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000066364 | 1-306 | Type 3 secretion system translocon protein SctB | |||
Sequence: MTINIKTDSPIITTGSQLDAITTETVKQSGEIKKTEDTRHEAQAIKSSEASLSRSQVPELIKPSQGINVALLSKSQGDLNGTLSILLLLLELARKAREMGLQQRDIENKATITAQKEQVAEMVSGAKLMIAMAVVSGIMAATSTVASAFSIAKEVKIVKQEQILNSNIAGREQLIDTKMQQMSNIGDKAVSREDIGRIWKPEQVADQNKLALLDKEFRMTDSKANAFNAATQPLGQMANSAIQVHQGYSQAEVKEKEVNASIAANEKQKAEEAMNYNDNFMKDVLRLIEQYVSSHTHAMKAAFGVV |
Interaction
Subunit
The core secretion machinery of the T3SS is composed of approximately 20 different proteins, including cytoplasmic components, a base, an export apparatus and a needle (PubMed:30107569).
This subunit is involved in the formation of a pore, called the translocon, in host membrane (PubMed:12603736, PubMed:23297383, PubMed:25684661).
Interacts with YopB/SctE (PubMed:15784589).
Together with YopB/SctE, forms a multimeric integral membrane complex (By similarity).
The C-terminal region interacts with LcrH (PubMed:12153562).
This subunit is involved in the formation of a pore, called the translocon, in host membrane (PubMed:12603736, PubMed:23297383, PubMed:25684661).
Interacts with YopB/SctE (PubMed:15784589).
Together with YopB/SctE, forms a multimeric integral membrane complex (By similarity).
The C-terminal region interacts with LcrH (PubMed:12153562).
Structure
Family & Domains
Features
Showing features for compositional bias, region, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 29-44 | Basic and acidic residues | ||||
Sequence: SGEIKKTEDTRHEAQA | ||||||
Region | 29-57 | Disordered | ||||
Sequence: SGEIKKTEDTRHEAQAIKSSEASLSRSQV | ||||||
Coiled coil | 31-44 | |||||
Sequence: EIKKTEDTRHEAQA | ||||||
Coiled coil | 253-273 | |||||
Sequence: VKEKEVNASIAANEKQKAEEA |
Domain
The first five codons form a minimal N-terminal secretion signal (PubMed:21965570).
The N-terminal region may also incorporate mechanisms of yopD/sctB translation control (PubMed:21965570).
An intact putative N-terminal coiled-coil might be critical for full virulence of Y.pseudotuberculosis (PubMed:22910185).
The central region (amino acids 150-227) plays a specific role in facilitating translocation of Yop proteins inside host cells that is genetically separable from its role in forming pores on host cell membranes (PubMed:25684661).
The N-terminal region may also incorporate mechanisms of yopD/sctB translation control (PubMed:21965570).
An intact putative N-terminal coiled-coil might be critical for full virulence of Y.pseudotuberculosis (PubMed:22910185).
The central region (amino acids 150-227) plays a specific role in facilitating translocation of Yop proteins inside host cells that is genetically separable from its role in forming pores on host cell membranes (PubMed:25684661).
Sequence similarities
Belongs to the SctB/YopD family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length306
- Mass (Da)33,387
- Last updated2004-11-23 v2
- Checksum4C0F0E9BCA215DA2
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 29-44 | Basic and acidic residues | ||||
Sequence: SGEIKKTEDTRHEAQA | ||||||
Sequence conflict | 183 | in Ref. 1; AAA72322 | ||||
Sequence: S → G |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L06215 EMBL· GenBank· DDBJ | AAA72322.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX936399 EMBL· GenBank· DDBJ | CAF25397.1 EMBL· GenBank· DDBJ | Genomic DNA |