Q06053 · DUS3_YEAST

Function

function

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs (PubMed:12003496, PubMed:14970222).
Specifically modifies U47 in cytoplasmic tRNAs (PubMed:14970222).
Catalyzes the synthesis of dihydrouridine in some mRNAs, thereby affecting their translation (By similarity).

Catalytic activity

Cofactor

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site299-301FMN (UniProtKB | ChEBI)
Binding site354FMN (UniProtKB | ChEBI)
Active site386Proton donor
Binding site426FMN (UniProtKB | ChEBI)
Binding site457FMN (UniProtKB | ChEBI)
Binding site509-511FMN (UniProtKB | ChEBI)
Binding site534-535FMN (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentnuclear periphery
Cellular Componentnucleus
Molecular Functionflavin adenine dinucleotide binding
Molecular Functionmetal ion binding
Molecular FunctionmRNA binding
Molecular FunctionmRNA dihydrouridine synthase activity
Molecular FunctiontRNA dihydrouridine synthase activity
Molecular FunctiontRNA-dihydrouridine47 synthase activity
Biological ProcessmRNA processing
Biological ProcesstRNA modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-dihydrouridine(47) synthase [NAD(P)(+)]
  • EC number
  • Alternative names
    • mRNA-dihydrouridine synthase DUS3
      (EC:1.3.1.-
      ) . EC:1.3.1.- (UniProtKB | ENZYME | Rhea)
    • tRNA-dihydrouridine synthase 3

Gene names

    • Name
      DUS3
    • ORF names
      L8084.19
    • Ordered locus names
      YLR401C

Organism names

Accessions

  • Primary accession
    Q06053
  • Secondary accessions
    • D6VZ35
    • P39519

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001621551-668tRNA-dihydrouridine47 synthase [NAD(P)+]

Proteomic databases

PTM databases

Interaction

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for compositional bias, region, zinc finger.

TypeIDPosition(s)Description
Compositional bias1-15Basic and acidic residues
Region1-31Disordered
Region59-92Disordered
Zinc finger92-122C3H1-type 1
Zinc finger134-164C3H1-type 2
Compositional bias227-242Basic and acidic residues
Region227-264Disordered

Sequence similarities

Belongs to the Dus family. Dus3 subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    668
  • Mass (Da)
    76,596
  • Last updated
    2008-04-29 v2
  • Checksum
    0DA116A41699E9C9
MEQNAEKRSIVGDDNSTVKRQDTSPSKGIAHIKPEYIVPLKQNENQKVAIYDEEMSSDRMTNEFAGGTNKKNKNGRGKKRGQNKNRDNRQVKEQNVLCPRLIHGDISKCSFGDNCRFVHDINLYLSTKKPEVESNIFPSCPVFNSLGFCPMGFKCRFLSSHLNKEDNILISKKEIDPDAQTIWSVKGEVNHISPERKLDLIKRRFPFTKSNEILEIIDSFQQECRDSMKPEEEVESTPQLKKQDPDVEQPVAPQVEQRNKELSEHRMKQREVYLKYKDTRYFAQEKKPLDLYHKKIVSPLTTVGNLPYRRLMRKLGADVTYSEMALAVPLIQGTNSEWALPKAHTSEFPGFGVQVACSKAWQAAKAAEALANSVSEISEINLNSGCPIDLLYRQGSGSALLDNPARMIRCLNAMNYVSKDIPITVKIRTGTKEGHPIAEGLVKRLVNETDVAAITLHGRSRQQRYTKSADWDYVSQVADTLRSAEADFIETEQGKEGRDSKNRIQFVGNGDVNNFEDWYRYLNGNENIDSVMVARGALIKPWIFEEVESQQYLDKTSTERLDILRDYAQFSMEHWGTDEYGISQCRRFFCEFMSFFHRYVPMGICERYPVKLNERPPNWCGRDELETLMGSTDVNDWIKLSDLFFGKTDENFVFVPKHKSSSYANRDS

Sequence caution

The sequence AAB82358.1 differs from that shown. Reason: Frameshift
The sequence CAA79376.1 differs from that shown. Reason: Frameshift

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-15Basic and acidic residues
Compositional bias227-242Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U19729
EMBL· GenBank· DDBJ
AAB82358.1
EMBL· GenBank· DDBJ
Genomic DNA Frameshift
Z18944
EMBL· GenBank· DDBJ
CAA79376.1
EMBL· GenBank· DDBJ
Genomic DNA Frameshift
BK006945
EMBL· GenBank· DDBJ
DAA09701.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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