Q05513 · KPCZ_HUMAN
- ProteinProtein kinase C zeta type
- GenePRKCZ
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids592 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).
Isoform 2
Catalytic activity
- L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H+
Activity regulation
Isoform 2: Constitutively active (By similarity).
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 258-266 | ATP (UniProtKB | ChEBI) | |||
Binding site | 281 | ATP (UniProtKB | ChEBI) | |||
Active site | 376 | Proton acceptor | |||
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameProtein kinase C zeta type
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ05513
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Associates with endosomes (PubMed:9566925).
Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction (PubMed:7597083).
Colocalizes with VAMP2 and WDFY2 in intracellular vesicles (PubMed:17313651).
Transiently translocates to the membrane of CA1 hippocampal cells in response to the induction of long term potentiation (By similarity).
Isoform 2
Keywords
- Cellular component
Disease & Variants
Features
Showing features for mutagenesis, natural variant.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 19 | No effect on interaction with SQSTM1 and PARD6B. | |||
Natural variant | VAR_050560 | 49 | in dbSNP:rs35271800 | ||
Mutagenesis | 62 | Loss of interaction with SQSTM1 and PARD6B. | |||
Mutagenesis | 66 | Loss of interaction with SQSTM1 and PARD6B. | |||
Natural variant | VAR_042310 | 84 | in dbSNP:rs56017162 | ||
Natural variant | VAR_035467 | 514 | in a colorectal cancer sample; somatic mutation | ||
Natural variant | VAR_042311 | 519 | in a colorectal adenocarcinoma sample; somatic mutation; dbSNP:rs376894109 | ||
Variants
![](/variants.8e7f84.jpg)
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 537 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data), modified residue.
Type | ID | Position(s) | Source | Description | ||
---|---|---|---|---|---|---|
Chain | PRO_0000055701 | 1-592 | UniProt | Protein kinase C zeta type | ||
Modified residue (large scale data) | 190 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 223 | PRIDE | Phosphoserine | |||
Modified residue | 410 | UniProt | Phosphothreonine; by PDPK1 and PI3K | |||
Modified residue | 560 | UniProt | Phosphothreonine | |||
Modified residue (large scale data) | 560 | PRIDE | Phosphothreonine | |||
Modified residue | 591 | UniProt | Phosphoserine | |||
Modified residue (large scale data) | 591 | PRIDE | Phosphoserine | |||
Post-translational modification
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Gene expression databases
Organism-specific databases
Interaction
Subunit
Interacts with PARD6A, PARD6B, PARD6G and SQSTM1. Part of a complex with PARD3, PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with ADAP1/CENTA1. Forms a ternary complex composed of SQSTM1 and PAWR. Interacts directly with SQSTM1 (Probable). Interacts with IKBKB. Interacts (via the protein kinase domain) with WWC1. Forms a tripartite complex with WWC1 and DDR1, but predominantly in the absence of collagen. Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1. Interacts with PDPK1 (via N-terminal region). Interacts with WDFY2 (via WD repeats 1-3) (PubMed:16792529).
Interacts with VAMP2 (PubMed:17313651).
Forms a complex with WDFY2 and VAMP2 (PubMed:17313651).
Interacts with APPL1 (PubMed:26583432).
Interacts with WWC1, WWC2 and WWC3 (PubMed:24682284).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | Q05513 | AGER Q15109 | 6 | EBI-295351, EBI-1646426 | |
BINARY | Q05513 | AKT1 P31749 | 2 | EBI-295351, EBI-296087 | |
BINARY | Q05513 | BCL10 O95999 | 3 | EBI-295351, EBI-958922 | |
BINARY | Q05513 | C1QBP Q07021 | 4 | EBI-295351, EBI-347528 | |
BINARY | Q05513 | HSP90AB1 P08238 | 2 | EBI-295351, EBI-352572 | |
BINARY | Q05513 | NCF1 P14598 | 3 | EBI-295351, EBI-395044 | |
BINARY | Q05513 | NUMB P49757-1 | 4 | EBI-295351, EBI-7199607 | |
XENO | Q05513 | numb P16554 | 2 | EBI-295351, EBI-429581 | |
BINARY | Q05513 | PARD6A Q9NPB6 | 10 | EBI-295351, EBI-81876 | |
BINARY | Q05513 | PARD6B Q9BYG5 | 18 | EBI-295351, EBI-295391 | |
BINARY | Q05513 | PARD6G Q9BYG4 | 9 | EBI-295351, EBI-295417 | |
BINARY | Q05513 | PRKCI P41743 | 10 | EBI-295351, EBI-286199 | |
BINARY | Q05513 | PRKCQ Q04759 | 3 | EBI-295351, EBI-374762 | |
BINARY | Q05513 | VHL P40337 | 3 | EBI-295351, EBI-301246 |
Complex viewer
Protein-protein interaction databases
Chemistry
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region, zinc finger.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 15-98 | PB1 | |||
Region | 79-145 | Interaction with SQSTM1 | |||
Zinc finger | 130-180 | Phorbol-ester/DAG-type | |||
Domain | 252-518 | Protein kinase | |||
Domain | 519-590 | AGC-kinase C-terminal | |||
Domain
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative promoter usage & Alternative splicing.
Q05513-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length592
- Mass (Da)67,660
- Last updated2005-06-21 v4
- MD5 ChecksumB70CA5932565C6B507515B708FA01636
Q05513-2
- Name2
- SynonymsPKMzeta
- NoteProduced by alternative promoter usage.
- Differences from canonical
- 1-183: Missing
Q05513-3
- Name3
- Differences from canonical
- 1-112: MPSRTGPKMEGSGGRVRLKAHYGGDIFITSVDAATTFEELCEEVRDMCRLHQQHPLTLKWVDSEGDPCTVSSQMELEEAFRLARQCRDEGLIIHVFPSTPEQPGLPCPGEDK → MLTPRTDE
Computationally mapped potential isoform sequences
There are 15 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
J3KRP7 | J3KRP7_HUMAN | PRKCZ | 61 | ||
E9PBE1 | E9PBE1_HUMAN | PRKCZ | 347 | ||
D6RJG4 | D6RJG4_HUMAN | PRKCZ | 73 | ||
D6RG01 | D6RG01_HUMAN | PRKCZ | 82 | ||
D6REZ8 | D6REZ8_HUMAN | PRKCZ | 65 | ||
D6RGG1 | D6RGG1_HUMAN | PRKCZ | 109 | ||
D6RBD4 | D6RBD4_HUMAN | PRKCZ | 98 | ||
D6RAU1 | D6RAU1_HUMAN | PRKCZ | 136 | ||
D6RAN5 | D6RAN5_HUMAN | PRKCZ | 70 | ||
D6RDM0 | D6RDM0_HUMAN | PRKCZ | 73 | ||
D6RCN4 | D6RCN4_HUMAN | PRKCZ | 25 | ||
D6RD31 | D6RD31_HUMAN | PRKCZ | 21 | ||
D6RC84 | D6RC84_HUMAN | PRKCZ | 46 | ||
F2Z3C5 | F2Z3C5_HUMAN | PRKCZ | 181 | ||
F2Z2H9 | F2Z2H9_HUMAN | PRKCZ | 105 |
Sequence caution
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Alternative sequence | VSP_046347 | 1-112 | in isoform 3 | ||
Alternative sequence | VSP_041904 | 1-183 | in isoform 2 | ||
Sequence conflict | 6 | in Ref. 1; AAA36488 | |||
Sequence conflict | 198 | in Ref. 3; BAF83684 | |||
Sequence conflict | 262 | in Ref. 1; AAA36488 | |||
Sequence conflict | 281 | in Ref. 3; BAH11883 | |||
Sequence conflict | 366 | in Ref. 3; BAF83684 | |||
Sequence conflict | 500 | in Ref. 3; BAF83684 | |||
Sequence conflict | 522 | in Ref. 3; BAF83684 | |||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L14283 EMBL· GenBank· DDBJ | AAA36488.1 EMBL· GenBank· DDBJ | mRNA | ||
BT007082 EMBL· GenBank· DDBJ | AAP35745.1 EMBL· GenBank· DDBJ | mRNA | ||
AK290995 EMBL· GenBank· DDBJ | BAF83684.1 EMBL· GenBank· DDBJ | mRNA | ||
AK294649 EMBL· GenBank· DDBJ | BAH11833.1 EMBL· GenBank· DDBJ | mRNA | ||
AK294782 EMBL· GenBank· DDBJ | BAH11883.1 EMBL· GenBank· DDBJ | mRNA | ||
AL162271 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL391845 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL590822 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL645703 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC008058 EMBL· GenBank· DDBJ | AAH08058.1 EMBL· GenBank· DDBJ | mRNA | ||
BC014270 EMBL· GenBank· DDBJ | AAH14270.1 EMBL· GenBank· DDBJ | mRNA | ||
Z15108 EMBL· GenBank· DDBJ | CAA78813.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |